Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds

Liu, Chaoxu; Abedian, Reza; Meister, Roland; Haasper, Carl; Hurschler, Christof; Krettek, Christian; Lewinski, Gabriela von; Jagodzinski, Michael

doi:10.1016/j.biomaterials.2011.10.041

Cited by 96 publications

(103 citation statements)

References 68 publications

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“…However, direct measurement of fluid-induced shear stress within scaffolds is not feasible, which prevents researchers from correlating the levels of fluid shear 5 stress to biochemical responses, see in particular (Gomes et al 2003;Jagodzinski et al 2008;Liu et al 2012a) and Table 1. Therefore, researchers are required to employ analytical predictions, based on idealised flow through a cylinder or two plates (Blecha et al 2010;Goldstein et al 2001), or estimate wall shear stress (WSS) magnitudes from existing computational models (Bancroft et al 2002;Grayson et al 2008;Vance et al 2005;Yu et al 2004), see Table 1.…”

Section: Introductionmentioning

confidence: 99%

“…when compared to applied fluid perfusion only (Jagodzinski et al 2008;Liu et al 2012a;Stops et al 2010). Interestingly, recent computational studies showed 7 that a combination of fluid perfusion and mechanical compression led to more ubiquitous stimulation of bone cells within a TE scaffold (Hendrikson et al 2014;Zhao et al 2015), in particular to both cells that had an attached and bridged configuration.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures

Zhao

Vaughan

McNamara

2015

Biomech Model Mechanobiol

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures

Zhao

Vaughan

McNamara

2015

Biomech Model Mechanobiol

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“…Perfusion also permits mechanical stimulation of the cells by means of shear stress (for a review see McCoy and O'Brien, 2010). These bioreactors can be improved with the addition of a compression system dissociated from or combined with perfusion (Liu et al, 2012), which is particularly useful and relevant in bone cell studies (e.g. analysis of mechanotransduction (Sittichockechaiwut et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Bouet¹,

Cruel²,

Laurent³

et al. 2015

eCM

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An engineered three dimensional (3D) in vitro cell culture system was designed with the goal of inducing and controlling in vitro osteogenesis in a reproducible manner under conditions more similar to the in vivo bone microenvironment than traditional two-dimensional (2D) models. This bioreactor allows efficient mechanical loading and perfusion of an original cubic calcium phosphate bioceramic of highly controlled composition and structure. This bioceramic comprises an internal portion containing homogeneously interconnected macropores surrounded by a dense layer, which minimises fluid flow bypass around the scaffold. This dense and flat layer permits the application of a homogeneous loading on the bioceramic while also enhancing its mechanical strength. Numerical modelling of constraints shows that the system provides direct mechanical stimulation of cells within the scaffold. Experimental results establish that under perfusion at a steady flow of 2 µL/min, corresponding to 3 ≤ Medium velocity ≤ 23 µm/s, mouse calvarial cells grow and differentiate as osteoblasts in a reproducible manner, and lay down a mineralised matrix. Moreover, cells respond to mechanical loading by increasing C-fos expression, which demonstrates the effective mechanical stimulation of the culture within the scaffold. In summary, we provide a "proof-of-concept" for osteoblastic cell culture in a controlled 3D culture system under perfusion and mechanical loading. This model will be a tool to analyse bone cell functions in vivo, and will provide a bench testing system for the clinical assessment of bioactive bone-targeting molecules under load.

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“…Hydrodynamic advantages that are observed in bioreactor cultures of tissueengineered bones have led to a significant assistance to this field (Rauh et al 2011;Yeatts & Fisher 2011;Salter et al 2012;Yu et al 2004;Liu et al 2012). Although many experiments and theories have shown that the functionality and quality of cultured cells and engineered tissues in bioreactors can be improved by mechanical stimulation, research on the direct impact of mechanical stimulation on cells or tissues, such as the magnitude, frequency, continuity, and cyclical changes of the mechanical stimuli, are not well characterized (Yeatts & Fisher 2011;Yu et al 2004;Martin et al 2004;Song et al 2006;Hu and Athanasiou 2005;Massimo et al 2004;Van Dyke et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects

Song

Wang

Zhang

et al. 2013

Cell Stress and Chaperones

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In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects.

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Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds

Cited by 96 publications

References 68 publications

Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures

Quantification of fluid shear stress in bone tissue engineering scaffolds with spherical and cubical pore architectures

Validation of an in vitro 3D bone culture model with perfused and mechanically stressed ceramic scaffold

Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects

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