Influence of Phospholipids Structure on the Physicochemical Properties and In Vitro Digestibility of Lactoferrin-Loaded Liposomes

Zhang, Jiawei; Han, Jianzhong; Ye, Aiqian; Li, Weilin; Tian, Mengmeng; Lu, Yujie; Wu, Kunrong; Liu, Jing; Lou, Ma Peizhan

doi:10.1007/s11483-019-09581-3

Cited by 23 publications

(16 citation statements)

References 49 publications

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“…In our analysis, we used formulations containing bLf embedded in liposomes. The inclusion of bLf in preserving structures, such as liposomes, reduces gastric and intestinal enzymatic digestion while maintaining its integrity and, therefore, its biological functionality [77,78]. Indeed, the bLf at 5% of iron saturation form is best suited to obtain the maximum chelating effect.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin as Antiviral Treatment in COVID-19 Management: Preliminary Evidence

Campione

Lanna

Cosio

et al. 2021

IJERPH

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Lactoferrin (Lf), a multifunctional cationic glycoprotein synthesized by exocrine glands and neutrophils, possesses an in vitro antiviral activity against SARS-CoV-2. Thus, we conducted an in vivo preliminary study to investigate the antiviral effect of oral and intranasal liposomal bovine Lf (bLf) in asymptomatic and mild-to-moderate COVID-19 patients. From April 2020 to June 2020, a total of 92 mild-to-moderate (67/92) and asymptomatic (25/92) COVID-19 patients were recruited and divided into three groups. Thirty-two patients (14 hospitalized and 18 in home-based isolation) received only oral and intranasal liposomal bLf; 32 hospitalized patients were treated only with standard of care (SOC) treatment; and 28, in home-based isolation, did not take any medication. Furthermore, 32 COVID-19 negative, untreated, healthy subjects were added for ancillary analysis. Liposomal bLf-treated COVID-19 patients obtained an earlier and significant (p < 0.0001) SARS-CoV-2 RNA negative conversion compared to the SOC-treated and untreated COVID-19 patients (14.25 vs. 27.13 vs. 32.61 days, respectively). Liposomal bLf-treated COVID-19 patients showed fast clinical symptoms recovery compared to the SOC-treated COVID-19 patients. In bLf-treated patients, a significant decrease in serum ferritin, IL-6, and D-dimers levels was observed. No adverse events were reported. These observations led us to speculate a potential role of bLf in the management of mild-to-moderate and asymptomatic COVID-19 patients.

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Section: Discussionmentioning

confidence: 99%

Lactoferrin as Antiviral Treatment in COVID-19 Management: Preliminary Evidence

Campione

Lanna

Cosio

et al. 2021

IJERPH

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“…The mixtures were incubated with agitation (Unitronic 320 P-Selecta, Barcelona, Spain) (30 rpm; at 37 °C); 200 µL of the sample were taken for analysis after 1, 30, and 120 min. Particle size, polydispersity index (PDI), and ζ-potential of RP+ST LC liposomes in the gastric and intestinal fluid, and during gastric and intestinal digestion were followed in time, using a Zetasizer Nano ZS (series HT, Malvern Instrument, U.K.) at 25 °C; measurement conditions were defined according to Zhang et al (2019b).…”

Section: Stability Of Liposomes Under Gastric and Intestinal Digestionmentioning

confidence: 99%

An in vitro digestion study of encapsulated lactoferrin in rapeseed phospholipid–based liposomes

et al. 2020

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“…A thin-layer dispersion method combined with microfluidization was used to prepare nanoliposomes as described in our previous study. 21 L-α-Phosphatidylcholine and DHA were completely dissolved in absolute ethanol at 40 °C in a mass ratio of 2:1. The ethanol was then evaporated under vacuum using a rotary evaporator (55 °C, 40 rpm, RV10DS96, IKA, Germany).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Novel Nanoliposome Codelivered DHA and Anthocyanidin: Characterization, In Vitro Infant Digestibility, and Improved Cell Uptake

Zhao²,

et al. 2021

J. Agric. Food Chem.

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There are still many challenges in understanding the absorption and transport mechanism of liposomes in the gastrointestinal tract of infants, especially for liposome-coentrapped two or more substances. In this study, novel docosahexaenoic acid (DHA)–anthocyanidin-codelivery liposomes (DA-LPs) were fabricated and characterized, and their digestive and absorptive behaviors were evaluated using the in vitro infant digestive method combined with the Caco-2 cell model. The liposomal bilayer structure remained intact with the particles aggregated in simulated infant gastric fluid, while their phospholipid membrane underwent enzymatic lipolysis under simulated intestinal conditions. Compared to single substance-loaded liposomes (DHA- or anthocyanidin-loaded liposomes), the digested DA-LPs showed better cell viability, higher cellular uptake and membrane fluidity, and lower reactive oxygen species (ROS). It can be concluded that DA-LPs are promising carriers for simultaneously transporting hydrophobic and hydrophilic molecules and may be beneficial for improving nutrient absorption and alleviating intestinal stress oxidation.

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Influence of Phospholipids Structure on the Physicochemical Properties and In Vitro Digestibility of Lactoferrin-Loaded Liposomes

Cited by 23 publications

References 49 publications

Lactoferrin as Antiviral Treatment in COVID-19 Management: Preliminary Evidence

Lactoferrin as Antiviral Treatment in COVID-19 Management: Preliminary Evidence

An in vitro digestion study of encapsulated lactoferrin in rapeseed phospholipid–based liposomes

Novel Nanoliposome Codelivered DHA and Anthocyanidin: Characterization, In Vitro Infant Digestibility, and Improved Cell Uptake

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