Influence of physical properties and operating parameters on hydrodynamics in Centrifugal Partition Chromatography

Countercurrent separation (CCS) utilizes the differential partitioning behavior of analytes between two immiscible liquid phases. We introduce the first platform (“CherryOne”) capable of real-time monitoring, metering, and control of the dynamic liquid-liquid CCS process. Automated phase monitoring and volumetrics are made possible with an array of sensors, including the new permittivity-based Phase Metering Apparatus (PMA). Volumetric data for each liquid phase are converted into a dynamic real-time display of stationary phase retention (Sf) and eluent partition coefficients (K), which represent critical parameters of CCS reproducibility. When coupled with the elution-extrusion operational mode (EECCC), automated Sf and K determination empowers untargeted and targeted applications ranging from metabolomic analysis to preparative purifications.

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“…The sensitivity of an optical sensor may be enhanced by incorporating a dye into one of the phases. 18 …”

Section: Resultsmentioning

confidence: 99%

Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation

Pauli¹,

Pro²,

Chadwick³

et al. 2015

Anal. Chem.

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“…It has been shown that, for a commonly used rotor rotation speed (higher than 1000 rpm), the stationary phase retention is mostly affected by the mobile phase flow rate, i.e. it decreases with increasing mobile phase flow rate [9,11,13]. In order to determine the maximum mobile phase flow rate at which a stationary phase retention of 0.5 can be ensured, we studied the stationary phase retention in ascending and descending mode at different mobile phase flow rates.…”

Section: Stationary Phase Retentionmentioning

confidence: 99%

“…The visualization of the flow regimes inside the cells of a CPC column have helped to better understand the influence of the biphasic liquid system physical properties, the mobile phase flow rate and the rotation speed (centrifugal force) on the phase contact area [9,13,14]. The rotation speed is one of the parameters that enhance the column efficiency.…”

Section: Influence Of the Rotation Speed On The Scpc Separationmentioning

confidence: 99%

“…This means that the stationary phase retention, in ascending step and in descending step, should be 0.5. In general, the stationary phase retention is a function of the physical properties of the biphasic liquid system, the column geometry and material, the flow rate of the mobile phase, the centrifugal force (rotor rotation speed) and the operating mode (ascending or descending) [9][10][11][12]. The selected solvent system 14 shows an adequate density difference of the two phases (0.14 g cm -3 at 25°C) and a short settling time of 21 s at 25°C (data measured in our laboratory).…”

Section: Stationary Phase Retentionmentioning

confidence: 99%

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Sequential Centrifugal Partition Chromatography: A New Continuous Chromatographic Technology

2011

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In solid-support-free liquid-liquid chromatography, both phases can be used as a mobile phase and, moreover, their role and flow direction can be reversed during the course of the separation. This is the background of the concept of sequential centrifugal partition chromatography (sCPC) developed to perform a continuous separation in a cyclic manner. The feed is continuously introduced between two centrifugal partition chromatographic columns and two products are alternately collected at the opposite ends of the columns. In this work, a method is presented to select the operating parameters of an sCPC unit for the complete separation of a binary feed mixture. The method uses, as a basis, a restricted number of experiments needed to determine the distribution equilibrium of the components to be separated and to evaluate the column hydrodynamics. The method was validated experimentally for the separation of a binary mixture of hydroquinone and pyrocatechol.

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“…The stationary phase retention decreases with the increase in the mobile phase flow rate and to some extent with the increase in the rotational speed (centrifugal force) (Adelmann and Schembecker, 2011;Foucault et al, 1992aFoucault et al, ,b, 1994Ignatova et al, 2001;Ignatova and Sutherland, 2003;Marchal et al, 2000). The stationary phase retention decreases with the increase in the mobile phase flow rate and to some extent with the increase in the rotational speed (centrifugal force) (Adelmann and Schembecker, 2011;Foucault et al, 1992aFoucault et al, ,b, 1994Ignatova et al, 2001;Ignatova and Sutherland, 2003;Marchal et al, 2000).…”

Section: Stationary Phase Retention Efficiency and Resolutionmentioning

confidence: 99%

Sequential Centrifugal Partition Chromatography

Rodrigues¹,

Pereira²,

Minceva³

et al. 2015

Simulated Moving Bed Technology

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Influence of physical properties and operating parameters on hydrodynamics in Centrifugal Partition Chromatography

Cited by 41 publications

References 15 publications

Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation

Real-Time Volumetric Phase Monitoring: Advancing Chemical Analysis by Countercurrent Separation

Sequential Centrifugal Partition Chromatography: A New Continuous Chromatographic Technology

Sequential Centrifugal Partition Chromatography

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