Influence of sample collection and preanalytical sample processing on the analyses of biological markers in the European multicentre study IDEFICS

Peplies, Jenny; Günther, Kathrin; Bammann, Karin; Fraterman, Arno; Russo, Paola; Veidebaum, Toomas; Tornaritis, Michael; Vanaelst, Barbara; Mårild, Stefan; Molnár, Denés; Moreno, Luís A.; Ahrens, Wolfgang

doi:10.1038/ijo.2011.41

Cited by 34 publications

(27 citation statements)

References 22 publications

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“…24,25 In addition, a detailed description of blood processing, shipment, storage and quality management during the baseline survey has been published earlier. 26,27 The same procedures were applied during the second survey. The examinations were suspended during the summer holidays.…”

Section: Examinations and Crp Analysismentioning

confidence: 99%

C-reactive protein reference percentiles among pre-adolescent children in Europe based on the IDEFICS study population

et al. 2014

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on behalf of the IDEFICS consortium OBJECTIVES: C-reactive protein (CRP) is involved in a wide range of diseases. It is a powerful marker for inflammatory processes used for diagnostic and monitoring purposes. We aimed to establish reference values as data on the distribution of serum CRP levels in young European children are scarce. SUBJECTS: Reference values of high-sensitivity CRP concentrations were calculated for 9855 children aged 2.0-10.9 years, stratified by age and sex. The children were recruited during the population-based European IDEFICS study (Identification and prevention of Dietary-and lifestyle-induced health Effects in Children and infantS) with 18 745 participants recruited from 2007 to 2010. RESULTS: In 44.1 % of the children, CRP values were below or equal the detection limit of 0.2 mg/l. Median CRP concentrations showed a slight negative age trend in boys and girls, whereas serum CRP values were slightly higher in girls than in boys across all age groups. CONCLUSIONS: Our population-based reference values of CRP may guide paediatric practice as elevated values may require further investigation or treatment. Therefore, the presented reference values represent a basis for clinical evaluation and for future research on risk assessment of diseases associated with increased CRP levels among children.

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Section: Examinations and Crp Analysismentioning

confidence: 99%

C-reactive protein reference percentiles among pre-adolescent children in Europe based on the IDEFICS study population

et al. 2014

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“…All blood, serum, urine and saliva samples were transferred to a central biorepository to co-ordinate the laboratory analyses and to ensure standardised storage and handling of samples. 22 Physical activity. To monitor physical activity, children wore a uniaxial accelerometer (ActiGraph or ActiTrainer; ActiGraph, Pensacola, FL, USA) on a hip belt over 3 consecutive days including 1 weekend day.…”

Section: Measurements and Examinationsmentioning

confidence: 99%

The IDEFICS cohort: design, characteristics and participation in the baseline survey

et al. 2011

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Background: The European IDEFICS (Identification and prevention of dietary-and lifestyle-induced health effects in children and infants) study was set up to determine the aetiology of overweight, obesity and related disorders in children, and to develop and evaluate a tailored primary prevention programme. Objective: This paper focuses on the aetiological element of the multicentre study, the measures and examinations, sociodemographic characteristics of the study sample and proportions of participation. Design: Prospective cohort study with an embedded intervention study that started with a baseline survey in eight countries in [2007][2008]. Subjects and measurements: Baseline participants of the prospective cohort study were 16 224 children aged 2-9 years. Parents reported sociodemographic, behavioural, medical, nutritional and other lifestyle data for their children and families. Examinations of children included anthropometry, blood pressure, fitness, accelerometry, DNA from saliva and physiological markers in blood and urine. The built environment, sensory taste perception and other mechanisms of children's food choices and consumer behaviour were studied in subgroups. Results: Between 1507 and 2567, children with a mean age of 6.0 years and an even sex distribution were recruited from each country. Of them, 82% lived in two-parent families. The distribution of standardised income levels differed by study sample, with low-income groups being strongly represented in Cyprus, Italy and Germany. At least one 24-h dietary recall was obtained for two-thirds of the children. Blood pressure and anthropometry were assessed in more than 90%. A 3-day accelerometry was performed in 46%, motor fitness was assessed in 41%, cardiorespiratory fitness in 35% and B11% participated in taste perception tests. The proportion of children donating venous blood, urine and saliva was 57, 86 and 88%, respectively. Conclusion: The IDEFICS cohort provides valuable data to investigate the interplay of social, environmental, genetic, physiological and behavioural factors in the development of major diet-and lifestyle-related disorders affecting children at present.

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“…Details on the biological sampling procedures can be obtained from an earlier publication. 25 HOMA-IR was calculated as fasting insulin (μIU ml − 1 ) × fasting glucose (mmol l − 1 )/22.5.…”

Section: Laboratory Analysesmentioning

confidence: 99%

Percentiles of fasting serum insulin, glucose, HbA1c and HOMA-IR in pre-pubertal normal weight European children from the IDEFICS cohort

et al. 2014

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on behalf of the IDEFICS consortium OBJECTIVES: The aim of this study is to present age-and sex-specific reference values of insulin, glucose, glycosylated haemoglobin (HbA1c) and the homeostasis model assessment to quantify insulin resistance (HOMA-IR) for pre-pubertal children. METHODS: The reference population consists of 7074 normal weight 3-to 10.9-year-old pre-pubertal children from eight European countries who participated in at least one wave of the IDEFICS ('identification and prevention of dietary-and lifestyle-induced health effects in children and infants') surveys (2007)(2008)(2009)(2010) and for whom standardised laboratory measurements were obtained. Percentile curves of insulin (measured by an electrochemiluminescence immunoassay), glucose, HbA1c and HOMA-IR were calculated as a function of age stratified by sex using the general additive model for location scale and shape (GAMLSS) method. RESULTS: Levels of insulin, fasting glucose and HOMA-IR continuously show an increasing trend with age, whereas HbA1c shows an upward trend only beyond the age of 8 years. Insulin and HOMA-IR values are higher in girls of all age groups, whereas glucose values are slightly higher in boys. Median serum levels of insulin range from 17.4 and 13.2 pmol l − 1 in 3-< 3.5-year-old girls and boys, respectively, to 53.5 and 43.0 pmol l − 1 in 10.5-< 11-year-old girls and boys. Median values of glucose are 4.3 and 4.5 mmol l − 1 in the youngest age group and 49.3 and 50.6 mmol l − 1 in the oldest girls and boys. For HOMA-IR, median values range from 0.5 and 0.4 in 3-< 3.5-year-old girls and boys to 1.7 and 1.4 in 10.5-< 11-year-old girls and boys, respectively. CONCLUSIONS: Our study provides the first standardised reference values for an international European children's population and provides the, up to now, largest data set of healthy pre-pubertal children to model reference percentiles for markers of insulin resistance. Our cohort shows higher values of Hb1Ac as compared with a single Swedish study while our percentiles for the other glucose metabolic markers are in good accordance with previous studies.

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Influence of sample collection and preanalytical sample processing on the analyses of biological markers in the European multicentre study IDEFICS

Cited by 34 publications

References 22 publications

C-reactive protein reference percentiles among pre-adolescent children in Europe based on the IDEFICS study population

C-reactive protein reference percentiles among pre-adolescent children in Europe based on the IDEFICS study population

The IDEFICS cohort: design, characteristics and participation in the baseline survey

Percentiles of fasting serum insulin, glucose, HbA1c and HOMA-IR in pre-pubertal normal weight European children from the IDEFICS cohort

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