Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage

Gündoğan, Mustafa; Yeni, Deniz; Avdatek, Fatih; Fidan, Abdurrahman Fatih

doi:10.1016/j.anireprosci.2010.08.012

Cited by 61 publications

(50 citation statements)

References 57 publications

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“…This could be originated from our base extender include cysteine and this already have an antioxidant capacity. Our results agreed with previously published studies (3,14,24) Demircan et al (17) recently reported that incubating H2O2 and DCFH-DA in oxygenated physiological medium causes a significant increase in fluorescence intensity, and antioxidants can attenuate this increment. Thus, the effectiveness of antioxidant compounds against the H2O2-induced increase in fluorescence intensity was also tested in the medium.…”

Section: Discussionsupporting

confidence: 83%

“…However, we observed protective effects of BSA (5 mg/mL) on plasma membrane functional integrity and acrosome integrity. Plasma membrane and acrosome integrity values of BSA5 group had a good agreement with previous studies (22,24).…”

Section: Discussionsupporting

confidence: 80%

“…At the end of 72 h, motility value of BSA5 group was significantly higher than other groups and this motility level (56%) is appropriate for AI. Motility values of liquid stored ram semen ranged between 70% -84%, 60% -80%, 40% -78%, 30% -77% (0, 24, 48 and 72 h, respectively) in previous studies (14,23,24,34). Despite our study performed in non-breeding season, results of our study were in agreement with these values.…”

Section: Discussioncontrasting

confidence: 52%

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Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

ALÇAY

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The aim of the study was to evaluate different BSA doses in soybean-lecithin based extenders for liquid storage of ram semen. Pooled semen was divided into four equal volumes and diluted in a single-step dilution method with BSA supplemented (BSA5, BSA7.5, BSA10 groups supplemented 5mg/mL, 7.5mg/mL, 10mg/mL BSA respectively) and BSA-free (control) extenders. Semen samples were assessed for sperm motility, plasma membrane functional integrity using hypo-osmotic swelling test (HOST), damaged acrosome using FITC-Pisum sativum agglutinin (PSA-FITC) at 0, 24, 48 and 72h of storage at 5 °C. Antioxidant activities of groups at 0 and 72h were measured with glutathionine (GSH) concentration, membrane lipid peroxidation status was analyzed using the malondialdehyde (MDA) concentration. Antioxidant capacity of extender also was measured with 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assays. In the study, BSA (5mg/mL) supplementation has beneficial effect on ram sperm parameters at all evaluation times. In addition, the increasing doses of BSA supplementation has a negative effect on sperm parameters especially 48 and 72h of storage.Keywords: BSA, lecithin, ram semen, short-term storage.BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5 C sıcaklıkta muhafaza edilmesine olumlu etkisi Özet: Bu çalışmada, farklı dozlarda BSA ilave edilmiş lesitin bazlı sulandırıcının koç spermasının 5°C'ta kısa sureli saklanması üzerindeki etkisinin değerlendirilmesi amaçlandı. Çalışma amacıyla alınan spermalar pooling yapılarak dört eşit hacme bölündü ve 5mg/mL, 7.5mg/mL, 10mg/mL BSA ilave edilmiş (BSA5, BSA7.5, BSA10 sırasıyla) ve edilmemiş (kontrol) sulandırıcılarla sulandırıldı. Sulandırılmış spermanın sıcaklığı 5°C'a düştükten sonra 0, 24, 48 ve 72. saatlerde; motilite, plazma membran fonksiyonel bütünük [hipoozmotik şişme testi (HOST)] ve akrozomal hasar [FITC-Pisum sativum agglutinin (PSA-FITC)] muayeneleri yapıldı. Çalışmanın başlangıcı ve sonunda antioksidan aktivitenin ve lipid peroksidasyonun değerlendirilmesi için malondialdehyde (MDA) ve glutathionine (GSH) konsantrasyonları ölçüldü. Ayrıca 2,2-Diphenyl-1-picrylhydrazyl (DPPH) ve Dichloro-dihydro-fluorescein diacetate (DCFH-DA) yöntemleri ile her bir sulandırıcının antioksidan kapasiteleri değerlendirildi. Yapılan muayenelerin sonucunda; 5mg/mL BSA ilavesinin koç spermasının kısa süreli muhafazasında etkili olduğu gözlemlendi. Ayrıca artan BSA konsantrasyonunun spermanın soğutularak saklanmasında olumsuz etki oluşturduğu belirlendi. Anahtar sözcükler: BSA, kısa süreli muhafaza, koç sperması, lesitin.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 80%

Section: Discussioncontrasting

confidence: 52%

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Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

ALÇAY

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…Tradicionalmente, la concentración espermática y el volumen seminal se consideran parámetros de importancia para la mayoría de los estudios de inseminación artificial (22,23). La concentración y el volumen disminuyeron de forma significativa entre el primer y segundo eyaculado, resultado similar a lo reportado por otros autores en ovinos (2,3,6).…”

Section: Discussionunclassified

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

et al. 2017

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Cómo citar este artículo: CarvajalSerna M, Cortés-López HA, ManriquePerdomo C, Grajales-Lombana HA. Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano. Rev Med Vet. 2018;(18) ResumenEl objetivo del presente estudio fue evaluar el efecto del eyaculado, la raza y las colectas sobre los parámetros de calidad seminal y cinemática en 3 tipos raciales ovinos de lana bajo condiciones de trópico alto colombiano. Se colectó semen de 12 machos de la raza criollo, romney marsh y hampshire de 2 a 5 años de edad, con vagina artificial, durante 4 colectas con un tiempo de abstinencia de 3 d. Se realizó el análisis de la motilidad con ayuda de un sistema computarizado de análisis seminal (CASA) en combinación con una tinción de fluorescencia Hoeschst 33259 para el estudio de la viabilidad y la morfología. Se realizó un análisis de medidas repetidas en el tiempo con el paquete estadístico SAS, empleando el método Proc Mixed. No hubo diferencias significativas (p > 0,05) en el porcentaje de motilidad total y motilidad progresiva para el primer y segundo eyaculado, pero sí hubo diferencias (p < 0,05) para la concentración, volumen y viabilidad; el primer eyaculado fue significativamente superior al segundo, independiente de la raza y la colecta. Las variables de cinemática espermática relacionadas con el movimiento progresivo y la velocidad (ALH, VAP, LIN) no difieren entre eyaculados, pero sí hay un efecto racial (p > 0,05) en el que la raza criolla es superior en estas variables, lo que podría sugerir que las células espermáticas de esta raza tienen la capacidad de recorrer mayor distancia en menor tiempo, comparado con los espermatozoides de las otras dos razas, en las condiciones del presente experimento. of total motility and progressive motility for the first and second ejaculates, but there were differences (p < 0.05) for concentration, volume, and viability; the first ejaculate was significantly superior to the second, independent of breed and collection. Sperm kinematic variables related to progressive movement and speed (ALH, VAP, LIN) do not differ between ejaculates, but there is a racial effect (p > 0.05), since the Creole breed is superior in all these variables, which could suggest that the sperm cells of this breed have the ability to travel greater distance in less time, compared with sperm from the other two breeds, under the conditions of the present experiment.Keywords: seminal quality, kinematics, sperm, sheep.Avaliação dos parâmetros de qualidade seminal e cinemática espermática em três raças ovinas de lã em condições de trópico alto colombianoResumo O objetivo deste estudo foi avaliar o efeito da ejaculação, a raça e as coletas sobre os parâ-metros de qualidade seminal e cinemática em 3 tipos raciais ovinos de lã sob condições de trópico alto colombiano. Foram colhidas amostras de sêmen de 12 machos da raça crioulo, romney marsh e hampshire de 2 a 5 anos de idade, com vagina artificial, durante 4 coletas com um tem...

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“…For semen transport from collection stations to user farms, short-term liquid storage at 48C to 58C, rather than the generally recommended 158C can give acceptable pregnancy rates up to 24 h after semen collection (O'Hara et al, 2010). However, the recent study by Gundogan et al (2010) on DNA integrity and oxidative stress indicates that deterioration in sperm quality during low-temperature liquid storage is greater at a concentration suitable for intrauterine insemination (25 3 10 6 spermatozoa per ml) than cervical insemination (100 3 10 6 per ml). Of course, 24 h is still only a tiny fraction of the time that semen can be stored in female reptiles, amphibians, fish and birds, prompting Holt and Lloyd (2010) to express surprise that the mechanisms used by such species have not been investigated by those researching the long-term storage of fresh semen.…”

Section: Discussionmentioning

confidence: 99%

Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

Robinson

McKelvey²,

King

et al. 2011

Animal

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This review brings together research findings on cervical relaxation in the ewe and its pharmacological stimulation for enhancement of the penetration needed for transcervical insemination and embryo transfer. On the basis that the success of artificial insemination is the percentage of ewes lambing, a review is made of recent research aimed at understanding and minimising the sub-lethal effects of freezing and thawing on the viability of spermatozoa, their membrane integrity and their ability to migrate through cervical mucus, as these characteristics have a major influence on fertility, particularly when semen is deposited, artificially, in the os cervix. Milestones of achievement are given for transcervical intrauterine insemination, embryo recovery and transfer and the birth of lambs of pre-determined sex, firstly following intracytoplasmic sperm injection, then laparoscopic intrauterine insemination using highly diluted flow-cytometrically sorted fresh semen and subsequently by os cervix insemination using sexed semen that had been frozen and thawed. Diversity of research endeavour (applied, cellular, molecular), research discipline (anatomy, histology, immunology, endocrinology) and research focus (cell, tissue, organ, whole animal) is embraced within the review as each has significant contributions to make in advancing recent scientific findings from the laboratory into robust on-farm transcervical insemination and embryo transfer techniques.Keywords: ovine cervix, semen cryopreservation, artificial insemination, embryo recovery and transfer ImplicationsIn bringing together the results of recent research on the cryopreservation of ram semen and the relaxation of the ewe cervix, this review provides a basis for identifying opportunities for improving the efficiency of cervical insemination and transcervical embryo recovery and transfer in sheep genetic improvement programmes.

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Influence of sperm concentration on the motility, morphology, membrane and DNA integrity along with oxidative stress parameters of ram sperm during liquid storage

Cited by 61 publications

References 57 publications

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

Positive effect of BSA supplemented soybean lecithin based extender on liquid storage of ram semen at 5C

Evaluación de los parámetros de calidad seminal y cinemática espermática en tres razas ovinas de lana en condiciones de trópico alto colombiano

Traversing the ovine cervix – a challenge for cryopreserved semen and creative science

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