Influence of storage method on the viability, mycelial growth and pathogenicity of<i>Sclerotinia sclerotiorum</i>sclerotia

Pottinger, Brenda M.; Ridgway, Hayley J.; Bourdôt, G. W.; Stewart, Alison

doi:10.1071/ap08062

Cited by 5 publications

(1 citation statement)

References 12 publications

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“…Plugs (5mm) taken from H. maydiscolonized PDYA plate of one replicate of each storage treatment were transferred to the center of fresh PDYA plates with 4 plates/ storage treatment. Radial growth was measured after 5 days at 27°C (Pottinger et al, 2008).…”

Section: Radial Growth On Pdyamentioning

confidence: 99%

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

El-Naggar

Yassin

2021

Egyptian Journal of Phytopathology

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Two isolates of Harpophora maydis, the causal agent of maize late wilt, were evaluated for viability, morphological characters, radial growth and virulence using twenty storage methods for 3, 12 and 24 months. Colonized discs preserved in sterile distilled water at room temperature (RT) from 25 to 32°C (SDW.RT) was the best treatment since the two isolates were recovered from all samples during the storage periods, with 90, 90 and 82.5%, respectively with no negative effect on their radial growth, morphological characters and virulence compared with the original isolates. Also, storage in 30% glycerol at room temperature (G30%. RT) exhibited a good degree of survivability (from 60 to 90%) and the examined characters were not altered. Unfortunately, fungal isolates were not able to recover after three months of storage when preserved by the methods of potato dextrose yeast agar with paraffin oil at RT, G30%. 4°C, G30%. -20°C and silica gel at -20°C. Based on the statistical analysis of the obtained results, it could be concluded that SDW.RT is simple, reliable and economic preservation method for H. maydis, moreover preservation in G30%.RT could be used along with SDW.RT or as alternative method for the tested fungus.

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Section: Radial Growth On Pdyamentioning

confidence: 99%

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

El-Naggar

Yassin

2021

Egyptian Journal of Phytopathology

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Comprehensive Sclerotinia Stem Rot Screening of Soybean Germplasm Requires Multiple Isolates of Sclerotinia sclerotiorum

et al. 2017

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Sclerotinia sclerotiorum population variability directly affects Sclerotinia stem rot (SSR) resistance breeding programs. In the north-central United States, however, soybean germplasm selection has often involved only a single isolate. Forty-four S. sclerotiorum isolates from Illinois, Michigan, Minnesota, Nebraska, Wisconsin, Poland, and across 11 different host species were evaluated for variation in isolate in vitro growth, in vitro oxalate production, and in planta aggressiveness on the susceptible soybean ‘Williams 82’. Significant differences (P < 0.0001) were detected in isolate in planta aggressiveness, in vitro growth, and in vitro oxalate production. Furthermore, diverse isolate characteristics were observed within all hosts and locations of collection. Aggressiveness was not correlated to colony growth and was only weakly correlated (r = 0.26, P < 0.0001) to isolate oxalate production. In addition, the host or location of collection did not explain isolate aggressiveness. Isolate oxalic acid production, however, may be partially explained by the host (P < 0.05) and location (P < 0.01) of collection. Using a representative subset of nine S. sclerotiorum isolates and soybean genotypes exhibiting susceptible or resistant responses (determined using a single isolate), a significant interaction (P = 0.04) was detected between isolates and genotypes when SSR severity was evaluated. Our findings suggest that screening of S. sclerotiorum-resistant soybean germplasm should be performed with multiple isolates to account for the overall diversity of S. sclerotiorum isolates found throughout the soybean-growing regions of the United States.

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Identification of Soybean (Glycine max) Check Lines for Evaluating Genetic Resistance to Sclerotinia Stem Rot

et al. 2021

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Soybean production in the Upper Midwest of the United States is affected by Sclerotinia stem rot (SSR) caused by the fungal pathogen Sclerotinia sclerotiorum, and genetic resistance is an important management strategy for this disease. However, assessing genetic resistance to S. sclerotiorum is challenging, because a standardized method to examine resistance across genotypes is lacking. Using a panel of nine diverse S. sclerotiorum isolates, four soybean lines were assessed for reproducible responses to S. sclerotiorum infection. Significant differences in SSR severity were found across isolates (P < 0.01) and soybean lines (P < 0.01), including one susceptible, two moderately resistant, and one highly resistant line. These four validated lines were used to screen 11 other soybean genotypes to evaluate their resistance levels, and significant differences were found across genotypes (P < 0.01). Among these 11 genotypes, five commercial and public cultivars displayed high resistance and were assessed in field studies across the Upper Midwest growing region for their response to SSR and yield. These five cultivars resulted in low disease levels (P < 0.01) in the field which were consistent with greenhouse experiments. Yield was significantly different in fields with disease present (P < 0.01) and disease absent (P < 0.01), and the order of cultivar performance was consistent between environments where disease was present or absent suggesting resistance prevented yield loss to disease. This study suggests the use of a soybean check panel can accurately assess SSR resistance in soybean germplasm and aid in breeding and commercial soybean development.

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Influence of storage method on the viability, mycelial growth and pathogenicity ofSclerotinia sclerotiorumsclerotia

Cited by 5 publications

References 12 publications

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

Viability, Growth and Virulence of Harpophora maydis Isolates Preserved Under Different Storage Methods

Comprehensive Sclerotinia Stem Rot Screening of Soybean Germplasm Requires Multiple Isolates of Sclerotinia sclerotiorum

Identification of Soybean (Glycine max) Check Lines for Evaluating Genetic Resistance to Sclerotinia Stem Rot

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