In order to study the anchoring instability mechanism of surrounding rock in deep roadway, the failure mechanism of the bolt-anchoring agent interface was studied by simulating different strength rock mass and ground temperature environment, using C20, C40, and C60 strength concrete and steel pipe to simulate different surrounding rock strength environments. Indoor pull-out tests were carried out to study the pull-out load displacement relationship, ultimate pull-out force, residual anchoring force, the distribution law of axial stress and tangential stress along the bar, and the energy consumption value of drawing failure at 20, 50, and 70°C. The test results show that, with the decrease of surrounding rock strength or the increase of ambient temperature, the pull-out force, residual anchoring force, and energy consumption value of anchorage interface gradually decrease; under different axial forces, the axial force distribution of the rod body decreases exponentially from the anchoring end to the opposite end; and the shear stress transfers to the deep part of the anchor body with the increase of the load. According to the failure phenomenon of the specimen, the failure modes of the bolt bolt-anchorage agent interface can be divided into shear slip mode and shear expansion slip mode. The shear expansion slip formula of anchorage interface is derived. Using high-strength and temperature-resistant resin anchoring agent for comparative test, the rationality of the mechanism analysis is proved, which provides more clear guidance for the construction of anchor support.