Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil

Kleineidam, Kristina; Košmrlj, Kristina; Kublik, Susanne; Palmer, Iris; Pfab, Helena; Ruser, Reiner; Fiedler, Sabine; Schloter, Michael

doi:10.1016/j.chemosphere.2011.02.086

Cited by 120 publications

(53 citation statements)

References 31 publications

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“…Concerning the effects of DMPP addition to the abundance and activity of amoA, the arguments still existed. For instance, Kleineidam et al (2011) observed impaired abundance of AOB in soil compartments after DMPP addition, while Florio et al (2014) found that no significant changes in amoA gene copies could be detected in soil after DMPP addition, while the transcriptional activity revealed by amoA mRNA was impaired. The latter was suggested in this study.…”

Section: Evolution Of the Abundance Of Nitrogen Transformation Functimentioning

confidence: 99%

“…3,4-Dimethylpyrazole phosphate (DMPP) is one of the most highly effective NIs (Weiske et al 2001;Zerulla et al 2001). Experiments have been conducted to investigate the effects of DMPP addition on nitrogen transformation (Yu et al 2007), plant productivity (Yu et al 2014), nitrogen transformation functional genes (Kleineidam et al 2011;Florio et al 2014), and chemical parameters (Barneze et al 2014) in soil. Moreover, it has been demonstrated that the applications of DMPP to animal manure can reduce soil N 2 O emissions (Weiske et al 2001;Menéndez et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Influence of natural zeolite and nitrification inhibitor on organics degradation and nitrogen transformation during sludge composting

Zhang

Sui

et al. 2015

Environ Sci Pollut Res

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Sludge composting is one of the most widely used treatments for sewage sludge resource utilization. Natural zeolite and nitrification inhibitor (NI) are widely used during composting and land application for nitrogen conservation, respectively. Three composting reactors (A-the control, B-natural zeolite addition, and C-3,4-dimethylpyrazole phosphate (DMPP) addition) were established to investigate the influence of NI and natural zeolite addition on organics degradation and nitrogen transformation during sludge composting conducted at the lab scale. The results showed that, in comparison with the control, natural zeolite addition accelerated organics degradation and the maturity of sludge compost was higher, while the DMPP addition slowed down the degradation of organic matters. Meanwhile, the nitrogen transformation functional genes including those responses for nitrification (amoA and nxrA) and denitrification (narG, nirS, nirK, and nosZ) were quantified through quantitative PCR (qPCR) to investigate the effects of natural zeolites and DMPP addition on nitrogen transformation. Although no significant difference in the abundance of nitrogen transformation functional genes was observed between treatments, addition of both natural zeolite and DMPP increases the final total nitrogen content by 48.6 % and 23.1 %, respectively. The ability of natural zeolite for nitrogen conservation was due to the absorption of NH 3 by compost, and nitrogen conservation by DMPP was achieved by the source reduction of denitrification. Besides, it was assumed that the addition of natural zeolite and DMPP may affect the activity of these genes instead of the abundance.

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Section: Evolution Of the Abundance Of Nitrogen Transformation Functimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Influence of natural zeolite and nitrification inhibitor on organics degradation and nitrogen transformation during sludge composting

Zhang

Sui

et al. 2015

Environ Sci Pollut Res

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“…More importantly, no negative effect of DMPP on non-target microorganisms in soils has been reported (Tindaon et al 2012;Kong et al 2016). The inhibition of nitrification by DMPP was previously thought to be through indiscriminately binding to the complex of membrane-bound proteins and inactivating the AMOcontaining AOB (Kleineidam et al 2011;Benckiser et al 2013). This perception, however, was challenged by the recent studies of Dong et al (2013) and Florio et al (2014), which provided evidence that DMPP inhibited AOA as well.…”

Section: Introductionmentioning

confidence: 99%

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

Shi

et al. 2016

Biol Fertil Soils

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The application of the nitrification inhibitor, 3,4-dimethylpyrazole-phosphate (DMPP), is considered as an effective strategy to mitigate agricultural nitrogen loss. However, the inhibitory effect of DMPP on nitrification is variable and the importance of the soil microbial community composition to the variability is poorly understood. In this study, nine soils were collected across three land uses to investigate the impact of DMPP on nitrification and associated dynamics of ammonia oxidizers in a 28-day microcosm incubation. The results showed that the efficacy of DMPP at inhibiting net nitrification rates varied highly from no effect to 63.6 % during the first week of incubation. The abundance of ammonia-oxidizing bacteria (AOB), rather than ammoniaoxidizing archaea (AOA), was significantly correlated with nitrate concentrations across three land uses and significantly inhibited by DMPP addition. DMPP had higher efficacy in neutral and alkaline wheat and vegetable soils, compared with pasture soils. Canonical correspondence analysis suggested that soil pH was the most influential factor explaining the community composition of AOB and AOA in the collected soils. However, neither ammonium nitrate nor DMPP addition had a significant effect on the community composition of AOB or AOA during the incubation indicated by non-metric multidimensional scaling ordination. Taken together, our findings indicated that DMPP slowed nitrification by inhibiting the growth of AOB, and DMPP application affected the abundance of AOB more than the ammonia oxidizer community composition.

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“…In contrast, Di et al (2009) suggested that AOB rather than AOA dominate nitrification in nitrogen-rich grassland soils by application of DCD. In addition, AOA growth was not suppressed by DCD and even increased in root-rhizosphere complex (pH 5.5) of cauliflower when DMPP was applied (Kleineidam et al, 2011). Liquid formulation of DMPP was as effective as DCD on inhibiting AOB amoA copies in six New Zealand grassland soils (Di and Cameron, 2011).…”

Section: Introductionmentioning

confidence: 87%

“…Different effects of the ammonium sulphate and nitrification inhibitors on AOB and AOA showed they adapted to different habitats due to their distinct physiological property (Valentine, 2007;Kleineidam et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Laboratory study of the effects of nitrification inhibitors on the abundance of ammonia-oxidizing bacteria and archaea in a Hap-Ustic Luvisol

Gong¹,

Zhang²,

Wu³

et al. 2012

Afr. J. Microbiol. Res.

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Nitrification inhibitors (NIs) could slow down nitrification by suppressing ammonia oxidizers.Responses of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) to NIs were studied in grassland soil and acidic agricultural soil, but we know little about effects of NIs and NIs per se on AOA and AOB abundance in neutral agricultural soil. We aimed to investigate the effects of NIs, dicyandiamide (DCD) and 3,4-dimethylpyrazole phosphate (DMPP), on the dynamics of AOA and AOB populations in a Hap-Ustic Luvisol. Laboratory studies were installed to observe the effects of DCD and DMPP on the abundance of AOA and AOB. We determined the dynamics of AOA and AOB populations during 112 days by using real-time PCR targeting amoA genes. Soil mineral nitrogen contents and potential ammonia oxidation were measured. AOB growth was stimulated by ammonium addition and reached peak value at day 14 in treatment N. When ammonium was applied with DCD or DMPP, AOB amoA copies decreased significantly. In contrast to AOB, AOA showed no response to the addition of ammonium and the two NIs during 91 days, even when DCD and DMPP applied were double dose. Nitrification inhibition rates of DCD and DMPP were highest at day 14. Although potential ammonia oxidation was suppreseed at day 14, AOA populations were still not affected by DCD or DMPP per se. Our results suggest that AOA were not stimulated by large amounts of inorganic ammonium exogenous and were tolerant to DCD and DMPP in a neutral agricultural soil.

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Influence of the nitrification inhibitor 3,4-dimethylpyrazole phosphate (DMPP) on ammonia-oxidizing bacteria and archaea in rhizosphere and bulk soil

Cited by 120 publications

References 31 publications

Influence of natural zeolite and nitrification inhibitor on organics degradation and nitrogen transformation during sludge composting

Influence of natural zeolite and nitrification inhibitor on organics degradation and nitrogen transformation during sludge composting

Effects of 3,4-dimethylpyrazole phosphate (DMPP) on nitrification and the abundance and community composition of soil ammonia oxidizers in three land uses

Laboratory study of the effects of nitrification inhibitors on the abundance of ammonia-oxidizing bacteria and archaea in a Hap-Ustic Luvisol

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