Influence of vitrification on mouse metaphase II oocyte spindle dynamics and chromatin alignment

Gomes, Cristina Marques; Silva, Cristine Ane Silva E.; Acevedo, Nicole; Baracat, Edmund C.; Serafini, P.; Smith, Gary D.

doi:10.1016/j.fertnstert.2007.08.025

Cited by 66 publications

(54 citation statements)

References 49 publications

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“…Increased Mad2 might accelerate spindle recovery during warming. Several researchers demonstrated that b-tubulin is depolymerized and that chromatin remains condensed on the metaphase plate immediately after warming of vitrified MII oocytes (30). However, b-tubulin repolymerized within a 2-hour period and formed morphologically normal metaphase spindles with properly aligned chromatin (30).…”

Section: Genementioning

confidence: 99%

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Jee

Lee

et al. 2011

Fertility and Sterility

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Section: Genementioning

confidence: 99%

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Jee

Lee

et al. 2011

Fertility and Sterility

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“…This could explain why fewer oocytes vitrified at the MII stage displayed cortical ER clusters compared with fresh MII-stage oocytes, as the ER and microtubules are interdependent structures [54]. Some studies [4,55,56] have shown that, although microtubules are disrupted immediately after cryopreservation of MII-stage oocytes, they are able to reform after culturing at 378, such that morphologically normal meiotic spindles become apparent. In our study, we examined the ER in vitrified MII-stage oocytes 1-2 h after oocyte warming.…”

Section: Mouse Oocyte Vitrification 151mentioning

confidence: 99%

“…To date, attempts to produce high-quality embryos that develop to term following freezing and thawing of MII-stage oocytes have had limited success [3][4][5]. Reasons for this may include low permeability of the oocyte membrane to cryoprotectants, susceptibility of the meiotic spindle to cooling [5][6][7][8][9], and toxic effects of cryoprotectants that affect various aspects of the oocyte's physiology [3,7,10,11].…”

Section: Introductionmentioning

confidence: 99%

“…The two main methods that have been used for cryopreservation are slow freezing/thawing and ultrarapid freezing (vitrification). While both methods can yield high oocyte survival and fertilization rates following cryopreservation [4,6,10,[12][13][14], direct comparisons between slow and rapid freezing procedures have shown that vitrification is likely to be the more promising method [6,7,10,15,16]. Although survival and fertilization rates of vitrified eggs have improved over the past several years, this procedure has resulted in only ;300-500 live births to date [3,17,18], suggesting that cryopreserved oocytes are compromised in other ways that do not affect survival and fertilizability.…”

Section: Introductionmentioning

confidence: 99%

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Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

Lowther

Weitzman

Maier

et al. 2009

Biology of Reproduction

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Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase IIstage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we examined the effects of vitrifying GVstage mouse oocytes on cytoplasmic structure and on the ability to undergo cytoplasmic changes necessary for proper fertilization and early embryonic development. We examined the endoplasmic reticulum (ER) as one indicator of cytoplasmic structure, as well as the ability of oocytes to develop Ca 2+ release mechanisms following vitrification and in vitro maturation. Vitrified GV-stage oocytes matured in culture to metaphase II at a rate comparable to that of controls. These oocytes had the capacity to release Ca 2+ following injection of inositol 1,4,5-trisphosphate, demonstrating that Ca 2+ release mechanisms developed during meiotic maturation. The ER remained intact during the vitrification procedure as assessed using the lipophilic fluorescent dye DiI. However, the reorganization of the ER that occurs during in vivo maturation was impaired in oocytes that were vitrified before oocyte maturation. These results show that vitrification of GV-stage oocytes does not affect nuclear maturation or the continuity of the ER, but normal cytoplasmic maturation as assessed by the reorganization of the ER is disrupted. Deficiencies in factors that are responsible for proper ER reorganization during oocyte maturation could contribute to the low developmental potential previously reported in vitrified in vitro-matured oocytes.assisted reproductive technology, gamete biology, in vitro fertilization, meiosis

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“…Earlier studies suggest that high cooling rate and high cryoprotectant concentration alters the arrangement of chromosomes and microtubules of mouse oocytes [20,23]. It seems that higher concentrations of cryoprotectants may have toxic effects on oocyte structure [2,24,25] and as a result our attempts to quantify Mater and Hook1 transcripts were not successful.…”

Section: Discussionmentioning

confidence: 95%

The effects of vitrification on gene expression in mature mouse oocytes by nested quantitative PCR

Habibi

Farrokhi

Silva

et al. 2010

J Assist Reprod Genet

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Influence of vitrification on mouse metaphase II oocyte spindle dynamics and chromatin alignment

Cited by 66 publications

References 49 publications

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Effect of antifreeze protein supplementation in vitrification medium on mouse oocyte developmental competence

Maturation, Fertilization, and the Structure and Function of the Endoplasmic Reticulum in Cryopreserved Mouse Oocytes1

The effects of vitrification on gene expression in mature mouse oocytes by nested quantitative PCR

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