INFOGEST inter-laboratory recommendations for assaying gastric and pancreatic lipases activities prior to in vitro digestion studies

Gastric and small intestinal lipid digestion kinetics as affected by the gradual addition of lipases and bile salts

“…In case of chyme samples, Orlistat (100 mM in ethanol) was added to inhibit gastric lipase. In the case of digest samples, 4-bromophenylboronic (1 M in methanol) was added as well to inhibit both gastric and pancreatic lipase, respectively (Brodkorb et al, 2019;Grundy et al, 2021).…”

Studying semi-dynamic digestion kinetics of food: Establishing a computer-controlled multireactor approach

“…In the small intestinal phase, a distinction was made between the food containing lipids (Ensure® Plus) and the one with a negligible amount of lipids (lentils). For the Ensure® drink, an aliquot of the digest was inhibited by adding 4-bromophenylboronic (1 M in methanol) to inhibit pancreatic lipase (Grundy et al, 2021). Another aliquot was placed in a water batch (5 min, 98 °C) to inhibit proteases and amylase (Brodkorb et al, 2019).…”

In vitro gastric lipid digestion of emulsions with mixed emulsifiers: Correlation between lipolysis kinetics and interfacial characteristics

“…In this case, a scaled-down version of the INFOGEST protocol was employed exactly as described in our previous work (Infantes-Garcia, Verkempinck, Gonzalez-Fuentes, et al, 2021). In this aspect, a gastric lipase activity of 60 U/mL (tributyrin-based) was set in the gastric phase performed at pH 3 (Grundy et al, 2021). The gastric lipid digestion kinetics were evaluated by digesting independent samples (tubes) as a function of time (5; 10; 15; 30; 45; 60; 90; 120 min after enzyme addition).…”