INFOGEST static in vitro simulation of gastrointestinal food digestion

Brodkorb, André; Egger, Lotti; Alminger, Marie; Alvito, Paula; Assunção, Ricardo; Ballance, Simon; Bohn, Torsten; Bourlieu-Lacanal, Claire; Boutrou, Rachel; Carrière, Frédéric; Clemente, Alfonso; Corredig, Milena; Dupont, Didier; Dufour, Claire; Edwards, Catharina; Golding, Matthew; Karakaya, Sibel; Kirkhus, Bente; Feunteun, Steven Le; Lesmes, Uri; Macierzanka, Adam; Mackie, Alan; Martins, Carla; Marze, Sébastien; McClements, David Julian; Ménard, Olivia; Minekus, Mans; Portmann, Reto; Santos, Cláudia N.; Souchon, Isabelle; Singh, R. Paul; Vegarud, Gerd Elisabeth; Wickham, Martin; Weitschies, Werner; Recio, Isidra

doi:10.1038/s41596-018-0119-1

Cited by 2,661 publications

(1,723 citation statements)

References 107 publications

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“…The in vitro digestion assay was performed according to the standardized INFOGEST protocol [20,24] using rehydrated freeze-dried samples. The solutions for mouth (Simulated Saliva Fluid, SSF), stomach (Simulated Gastric Fluid, SGF), and small intestinal (Simulated Duodenal Fluid, SDF) compartments were prepared according to a previous article [25].…”

Section: In Vitro Digestion Assaymentioning

confidence: 99%

Digestive Stability and Bioaccessibility of Antioxidants in Prickly Pear Fruits from the Canary Islands: Healthy Foods and Ingredients

et al. 2020

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Although prickly pear fruits have become an important part of the Canary diet, their native varieties are yet to be characterized in terms of betalains and phenolic compounds. To exert potential health benefits, these antioxidants must be released from the food matrix and be stable in the gastrointestinal tract. Our aim was to characterize the betalain and phenolic profile of four prickly pear varieties from the Canary Islands (Spain) and determine their digestive stability and bioaccessibility via in vitro gastrointestinal digestion. Digestive studies were performed considering the (i) importance of the edible fraction (pulps) and (ii) potential of fruit peels as by-products to obtain healthy ingredients. Betalains and phenolic profiles were analyzed by HPLC-DAD-ESI/MS and HPLC-DAD-MS/QTOF. Pulps in Colorada and Fresa varieties presented high indicaxanthin and betanin content, respectively. Despite low pH in the gastric phase, betalains were stable to reach the intestinal phase, although indicaxanthin presented a higher bioaccessibility. Blanco Buenavista peels contained a distinct flavonoid profile including a new isorhamnetin-hexosyl-rhamnoside. Phenolic compounds were abundant and highly bioaccessible in fruit peels. These findings suggest that prickly pear pulps are rich in bioaccessible betalains; and that their peels could be proposed as potential by-products to obtain sustainable healthy ingredients.

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Section: In Vitro Digestion Assaymentioning

confidence: 99%

Digestive Stability and Bioaccessibility of Antioxidants in Prickly Pear Fruits from the Canary Islands: Healthy Foods and Ingredients

et al. 2020

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“…Bioaccessibility of the OP polyphenols was assessed by in vitro static simulation of gastrointestinal digestion in the upper tract following the standardized protocol described by Brodkorb and co-workers [27]. The procedure was consisted of two sequential incubations; initially in simulated gastric fluid (SGF)/pepsin/gastric lipase to simulated gastric conditions followed by simulated intestinal fluid (SIF)/bile salts/pancreatin to simulate duodenal digestion.…”

Section: In Vitro Simulated Gastrointestinal Digestionmentioning

confidence: 99%

Influence of Pomace Matrix and Cyclodextrin Encapsulation on Olive Pomace Polyphenols’ Bioaccessibility and Intestinal Permeability

2020

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Olive pomace is a rich source of biologically active compounds, mainly polyphenols. Recently, an efficient and sustainable cyclodextrin (CD)-enhanced extraction was developed. It enabled a relatively simple formulation of high-quality olive pomace extracts (OPEs) that can be used as alternative sources of olive-derived polyphenols in the nutrition and pharma industries. However, biological effects and nutraceutical potential of OPEs are primarily limited by generally low oral bioavailability of major polyphenols (hydroxytyrosol and its derivatives) that can be significantly influenced by OPE matrix and the presence of CDs in formulation. The major goal of this research was to investigate the impact of complex matrix and different types of CDs on gastrointestinal stability and intestinal permeability of major OPE polyphenols, and provide additional data about mechanisms of absorption and antioxidant activity in gut lumen. Obtained results showed high bioaccessibility but relatively low permeability of OPE polyphenols, which was negatively affected by OPE matrix. CDs improved antioxidant efficiency of tested OPEs and tyrosol gastrointestinal stability. Effects of CDs on permeability and the metabolism of particular OPE polyphenols were CD-and polyphenol-specific.Nutrients 2020, 12, 669 2 of 16 oxidative stress in the intestine is particularly important given that numerous studies have shown that oxidative stress is directly linked to the development of diseases of the GI tract, such as inflammatory bowel disease and tumor colon. The intestine is the site that was shown to be extremely susceptible to oxidative stress. Namely, lipid peroxidation (as a chain reaction that lead to deleterious cell membrane damage) can be initiated by dietary fats.Considering its phenolic composition, olive pomace is very similar to olive oil and represents an inexpensive and readily available source of HTS derivatives. In the last decade, the potential of the olive pomace as a rich source of biologically active compounds has been recognized in scientific and professional literature, resulting in the development of a large number of effective extraction procedures [6,7]. However, the poor technological properties of raw olive pomace extracts (OPE) limit their wider application as nutraceuticals and food additives [7]. The main prerequisites for obtaining usable OPE are high yield of HTS derivatives, stability, and satisfactory technological properties of dry extracts. In this regard, cyclodextrins (CDs) were successfully applied for achieving higher polyphenol yields and the formulation of stable and organoleptically acceptable olive pomace extracts (OPEs) [7]. Moreover, α-CD, β-CD, and γ-CD are listed on the generally regarded as safe (GRAS) list of the U.S. Food and Drug Administration (FDA) for use as a food additive [8][9][10].In addition to demonstrating favorable chemical composition and technological properties, the quality of OPE is significantly determined by bioavailability and biological activity of its main active components...

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“…oral, gastric and duodenal phases). 3,4 However, this model suffers from some limitations. Gastric lipase, which is known to contribute to up to 25% (ref.…”

Section: Introductionmentioning

confidence: 99%

A standardised semi-dynamic in vitro digestion method suitable for food – an international consensus

et al. 2020

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INFOGEST static in vitro simulation of gastrointestinal food digestion

Cited by 2,661 publications

References 107 publications

Digestive Stability and Bioaccessibility of Antioxidants in Prickly Pear Fruits from the Canary Islands: Healthy Foods and Ingredients

Digestive Stability and Bioaccessibility of Antioxidants in Prickly Pear Fruits from the Canary Islands: Healthy Foods and Ingredients

Influence of Pomace Matrix and Cyclodextrin Encapsulation on Olive Pomace Polyphenols’ Bioaccessibility and Intestinal Permeability

A standardised semi-dynamic in vitro digestion method suitable for food – an international consensus

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