Information integration during bioelectric regulation of morphogenesis of the embryonic frog brain

Manicka, Santosh; Pai, Vaibhav P.; Levin, Michael

doi:10.1016/j.isci.2023.108398

Cited by 3 publications

(1 citation statement)

References 173 publications

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“…Bioelectric signaling possesses several unique properties that render them a prime subject for investigating bacterial–neuronal communication [ 38 , 39 , 40 , 41 ]. This is largely due to their operation across various levels of organization [ 42 , 43 , 44 , 45 , 46 , 47 ], where specific bioelectric states dictate properties at the cellular, organ, and organismal levels. The bioelectrical profile offers several advantages, such as susceptibility to modification by external stimuli, without the need for genetic or biochemical manipulation.…”

Section: Introductionmentioning

confidence: 99%

From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis

Bourqqia-Ramzi,

Mansilla-Guardiola,

Muñoz-Rodriguez

et al. 2024

IJMS

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The gut microbiome plays a fundamental role in metabolism, as well as the immune and nervous systems. Microbial imbalance (dysbiosis) can contribute to subsequent physical and mental pathologies. As such, interest has been growing in the microbiota–gut–brain brain axis and the bioelectrical communication that could exist between bacterial and nervous cells. The aim of this study was to investigate the bioelectrical profile (electrome) of two bacterial species characteristic of the gut microbiome: a Proteobacteria Gram-negative bacillus Escherichia coli (E. coli), and a Firmicutes Gram-positive coccus Enterococcus faecalis (E. faecalis). We analyzed both bacterial strains to (i) validate the fluorescent probe bis-(1,3-dibutylbarbituric acid) trimethine oxonol, DiBAC4(3), as a reliable reporter of the changes in membrane potential (Vmem) for both bacteria; (ii) assess the evolution of the bioelectric profile throughout the growth of both strains; (iii) investigate the effects of two neural-type stimuli on Vmem changes: the excitatory neurotransmitter glutamate (Glu) and the inhibitory neurotransmitter γ-aminobutyric acid (GABA); (iv) examine the impact of the bioelectrical changes induced by neurotransmitters on bacterial growth, viability, and cultivability using absorbance, live/dead fluorescent probes, and viable counts, respectively. Our findings reveal distinct bioelectrical profiles characteristic of each bacterial species and growth phase. Importantly, neural-type stimuli induce Vmem changes without affecting bacterial growth, viability, or cultivability, suggesting a specific bioelectrical response in bacterial cells to neurotransmitter cues. These results contribute to understanding the bacterial response to external stimuli, with potential implications for modulating bacterial bioelectricity as a novel therapeutic target.

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Section: Introductionmentioning

confidence: 99%

From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis

Bourqqia-Ramzi,

Mansilla-Guardiola,

Muñoz-Rodriguez

et al. 2024

IJMS

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Oscillatory phenomena in electrophysiological networks: The coupling between cell bioelectricity and transcription

Cervera,

Manzanares,

Levin

et al. 2024

Computers in Biology and Medicine

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Multicellular adaptation to electrophysiological perturbations analyzed by deterministic and stochastic bioelectrical models

Cervera,

Levin,

Mafe

2024

Sci Rep

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Cells can compensate a disruptive change in one ion channel by compensatory changes in other channels. We have simulated the adaptation of a multicellular aggregate of non-excitable cells to the electrophysiological perturbation produced by the external blocking of a cation channel. In the biophysical model employed, we consider that this blocking provokes a cell depolarization that opens a voltage-gated calcium channel, thus allowing toxic Ca2+ levels. The cell adaptation to this externally-induced perturbation is ascribed to the multiplicity of channels available to keep the cell membrane potential within a physiological window. We propose that the cell depolarization provokes the upregulated expression of a compensatory channel protein that resets the cell potential to the correct polarized value, which prevents the calcium entry. To this end, we use two different simulation algorithms based on deterministic and stochastic methods. The simulations suggest that because of the local correlations coupling the cell potential to transcription, short-term bioelectrical perturbations can trigger long-term biochemical adaptations to novel stressors in multicellular aggregates. Previous experimental data on planarian flatworms’ adaptation to a barium-containing environment is also discussed.

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Information integration during bioelectric regulation of morphogenesis of the embryonic frog brain

Cited by 3 publications

References 173 publications

From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis

From the Microbiome to the Electrome: Implications for the Microbiota–Gut–Brain Axis

Oscillatory phenomena in electrophysiological networks: The coupling between cell bioelectricity and transcription

Multicellular adaptation to electrophysiological perturbations analyzed by deterministic and stochastic bioelectrical models

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