Infrared linear dichroism as a tool to monitor antitumor drug-induced changes of the conformational flexibility of biopolymers

Fritzsche, H.

doi:10.1016/0022-2860(90)80068-u

Cited by 3 publications

(4 citation statements)

References 4 publications

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“…Pohle & Fritzsche (1990) have surveyed the potential of infra-red spectroscopy to determine conformations of nucleic acids, including the use of phosphate vibrations and the influence of drug molecules. Fritzsche (1990) has shown that the B to A transition, driven by the reduced water activity, is affected by the interaction of DNA with intercalating as well as nonintercalating antitumour drugs.…”

Section: Films Of Pure Substratementioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

360

376

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This review will consider solution studies of structure and interactions of DNA and DNA complexes using linear dichroism spectroscopy, with emphasis on the technique of orientation by flow. The theoretical and experimental background to be given may serve, in addition, as a general introduction into the state of the art of linear dichroism spectroscopy, particularly as it is applied to biophysical problems.

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Section: Films Of Pure Substratementioning

confidence: 99%

Linear dichroism spectroscopy of nucleic acids

Nordén

Kubista

Kurucsev

1992

Quart. Rev. Biophys.

360

376

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“…The somewhat characteristic geometries of the DNA backbone obtained from the studies discussed above have subsequently been used to investigate the effects of various drugs on DNA conformation. Fritzsche et al 18,[205][206][207][208][209] have published several such investigations, showing the ability of intercalating and nonintercalating drugs to inhibit DNA conformational transition. This was initially shown with p-IR by analysis of highly oriented DNA-ligand films produced by a wet-spinning method, the ligands utilized in this study being violamycin B1, L-carnosine, and netropsin.…”

Section: Nucleic Acidsmentioning

confidence: 99%

“…An observation of the correlation between the ability of a ligand to inhibit conformational transitions of DNA and the inhibition of DNA replication, lead to the proposal of a novel metric (the "freezing potential" or "freezing index") to predict the biological activity of future DNA targeted drugs. 206,208 The p-IR measurements of DNA appear to have declined in more recent times. However, the latest study utilizing p-IR to analyze DNA emphasizes the techniques' relevance for studying more complex DNA samples.…”

Section: Nucleic Acidsmentioning

confidence: 99%

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Linearly Polarized Infrared Spectroscopy for the Analysis of Biological Materials

Gassner

Vongsvivut

et al. 2023

Appl Spectrosc

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The analysis of biological samples with polarized infrared spectroscopy (p-IR) has long been a widely practiced method for the determination of sample orientation and structural properties. In contrast to earlier works, which employed this method to investigate the fundamental chemistry of biological systems, recent interests are moving toward “real-world” applications for the evaluation and diagnosis of pathological states. This focal point review provides an up-to-date synopsis of the knowledge of biological materials garnered through linearly p-IR on biomolecules, cells, and tissues. An overview of the theory with special consideration to biological samples is provided. Different modalities which can be employed along with their capabilities and limitations are outlined. Furthermore, an in-depth discussion of factors regarding sample preparation, sample properties, and instrumentation, which can affect p-IR analysis is provided. Additionally, attention is drawn to the potential impacts of analysis of biological samples with inherently polarized light sources, such as synchrotron light and quantum cascade lasers. The vast applications of p-IR for the determination of the structure and orientation of biological samples are given. In conclusion, with considerations to emerging instrumentation, findings by other techniques, and the shift of focus toward clinical applications, we speculate on the future directions of this methodology.

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Infrared spectroscopy as a tool for investigations of DNA structure and DNA - ligand interactions

Pohle

Fritzsche

1990

Journal of Molecular Structure

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Infrared linear dichroism as a tool to monitor antitumor drug-induced changes of the conformational flexibility of biopolymers

Cited by 3 publications

References 4 publications

Linear dichroism spectroscopy of nucleic acids

Linear dichroism spectroscopy of nucleic acids

Linearly Polarized Infrared Spectroscopy for the Analysis of Biological Materials

Infrared spectroscopy as a tool for investigations of DNA structure and DNA - ligand interactions

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