Inhalation therapy with M1 inhibits experimental melanoma development and metastases in mice

Andrade, Lucas Ferrari de; Mozeleski, Brian; Leck, Aline Raquell; Rossi, Gustavo Rodrigues; Costa, Cleber Rafael Vieira da; Guimarães, Fernando de Souza Fonseca; Zotz, Rafael; Nascimento, Katia Fialho do; Oliveira, Carolina Camargo de; Buchi, Dorly de Freitas; Trindade, Edvaldo da Silva

doi:10.1016/j.homp.2015.08.007

Cited by 12 publications

(16 citation statements)

References 35 publications

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“…For a number of years, scientists and physicians have considered that the anti-tumor effects of high dilutions were due solely to immune system activation. However, we have found that treatment with M1 in vivo did not affect immune cell numbers in metastatic melanoma-bearing mouse lungs (35). Additionally, the effects of M1 seem to occur irrespective of lymphocyte-mediated immunity (unpublished data).…”

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 84%

“…Finding cost-effective treatments for selected diseases has been a priority in our research group. Over the past years, we have investigated, in vitro (24)(25)(26)(27)(28)(29)(30)(31) and in vivo (32)(33)(34)(35)(36), the effects of highly diluted natural complexes (HDNCs). Lately, we have focused on two specific HDNCs, namely M1 and M8.…”

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

“…Anti-melanoma effects have been described for both HDNCs in melanoma-bearing mice. Lung colonization was shown to be attenuated in mice injected with B16-F10 melanoma cells, followed by M8 (36) or M1 (35) treatments. Decreased proliferation, combined with the induction of cell death were found in the tumor microenvironment of these M1-treated mice.…”

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

“…Decreased proliferation, combined with the induction of cell death were found in the tumor microenvironment of these M1-treated mice. In addition, tumor growth was impaired in solid B16-F10-derived subcutaneous tumors by a decrease in cell proliferation and angiogenesis, as well as by an enhancement of cell death (35). From these studies, we have been trying to elucidate the cellular mechanisms behind such effects.…”

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

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In vitro attenuation of classic metastatic melanoma‑related features by highly diluted natural complexes: Molecular and functional analyses

et al. 2019

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Metastasis is responsible for the majority of deaths among patients with malignant melanoma. Despite recent advances, the majority of current and modern therapies are ineffective and/or financially unfeasible. Thus, in this study, we investigated two low-cost highly-diluted natural complexes (HDNCs) that have been shown to be effective against malignant melanoma in a murine model in vivo. The aim of this study was to determine the mechanisms through which these HDNCs directly affect melanoma cells, either alone or in an artificial tumor microenvironment, suppressing the metastatic phenotype, thus explaining previous in vivo effects. For this purpose, HDNC in vitro treatments of B16-F10 melanoma cells, alone or in co-culture with Balb/3T3 fibroblasts, were carried out. Molecular biology techniques and standard functional assays were used to assess the changes in molecule expression and in cell behaviors related to the metastatic phenotype. Melanoma progression features were found to be regulated by HDNCs. Molecules related to cell adhesion (N-cadherin, β1-integrin and CD44), and migration, extracellular matrix remodeling and angiogenesis were modulated. The cell migratory, invasive and clonogenic capacities were reduced by the HDNCs. No loss of cell proliferation or viability were observed. On the whole, the findings of this study indicate that HDNCs directly reprogram, molecularly and functionally, melanoma cells in vitro, modulating their metastatic phenotype. Such findings are likely to be responsible for the attenuation of tumor growth and lung colonization previously observed in vivo.

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Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 84%

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

Section: In Vitro Attenuation Of Classic Metastatic Melanoma-related mentioning

confidence: 99%

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In vitro attenuation of classic metastatic melanoma‑related features by highly diluted natural complexes: Molecular and functional analyses

et al. 2019

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“…47 Antitumour effects, directly by killing tumour cells or acting via immune system activation, were demonstrated after experimental tumour-bearing mice treatment with 'Lymphomyosot', 48 Hydrastis canadensis, Lycopodium, Thuja, 49 Calcarea carbonica, 50 M8 complex, 20 and M1 complex. 51 Cell culture has also been used to demonstrate homeopathic preparations' cytotoxicity to tumour cells but not to normal cells. Anti-proliferative effects of Sarsaparilla on ACHN (human renal adenocarcinoma), Ruta graveolens on COLO-205 (human colorectal carcinoma), and Phytolacca decandra on MCF-7 (human breast carcinoma) cells were shown.…”

Section: Discussionmentioning

confidence: 99%