INHBA is a Prognostic Biomarker and Correlated With Immune Cell Infiltration in Cervical Cancer

Abstract

Background: Inhibin A (INHBA), a member of the TGF-β superfamily, has been shown to be differentially expressed in various cancer types and is associated with prognosis. However, its role in cervical cancer remains unclear.Methods: We aimed to demonstrate the relationship between INHBA expression and pan-cancer using The Cancer Genome Atlas (TCGA) database. Next, we validated INHBA expression in cervical cancer using the Gene Expression Omnibus (GEO) database, including GSE7803, GSE63514, and GSE9750 datasets. Enrichment analysis of INHBA was performed using the R package “clusterProfiler.” We analyzed the association between immune infiltration level and INHBA expression in cervical cancer using the single-sample gene set enrichment analysis (ssGSEA) method by the R package GSVA. We explored the association between INHBA expression and prognosis using the R package “survival”.Results: Pan-cancer data analysis showed that INHBA expression was elevated in 19 tumor types, including cervical cancer. We further confirmed that INHBA expression was higher in cervical cancer samples from GEO database and cervical cancer cell lines than in normal cervical cells. Survival prognosis analysis indicated that higher INHBA expression was significantly associated with reduced Overall Survival (p = 0.001), disease Specific Survival (p = 0.006), and Progression Free Interval (p = 0.001) in cervical cancer and poorer prognosis in other tumors. GSEA and infiltration analysis showed that INHBA expression was significantly associated with tumor progression and some types of immune infiltrating cells.Conclusion:INHBA was highly expressed in cervical cancer and was significantly associated with poor prognosis. Meanwhile, it was correlated with immune cell infiltration and could be used as a promising prognostic target for cervical cancer.

EREG, HOPX and SYNGR3 influence the Pathogenesis and Prognosis of Cervical Cancer through immune cells infiltration

“…Previous studies have shown that mRNA may affect the proliferation of cervical cancer cells through immune in ltrating cells [33]. Zhao et al demonstrated that the INHBA gene is a prognostic biomarker and correlated with immune cell in ltration in cervical cancer [34]. In our study, neutrophils were positively correlated with the EREG gene and the HOPX gene, but negatively correlated with the SYNGR3 gene, which is consistent with our previous analysis of prognosis.…”

Cellograph: A Semi-supervised Approach to Analyzing Multi-condition Single-cell RNA Sequencing Data Using Graph Neural Networks

“…Differential expression between the three clusters (Figure 3C) identified high expression of TUBA1B in cluster 5, which is associated with poor prognosis in NSCLC, suggesting persisting drug tolerance after the holiday period. Similarly, we observe differential expression of INHBA in cluster 3, a senescence mediator that’s associated with prognosis in many cancer types (Zhao et al, 2022). Interestingly, TUBA1B and INHBA expression are significantly reduced in the day 19 population that was not retreated with Erlotinib.…”

Long non-coding RNAs PGM5-AS1 upregulates Decorin (DCN) to inhibit cervical cancer progression by sponging miR-4284

“…GSE9750, an mRNA microarray, was used to identify key CC genes [ 46–48 ]. In previous studies, researchers used more than three microarrays from the GEO database, including GSE9750, to screen key genes in CC [ 47–49 ]. When performing different bioinformatic strategies in our study, we only used GSE9750, along with metascape for GO enrichment, and the TCGA database to identify that DCN was the key gene in CC; this result was different from those of previous studies.…”