Inheritance of polymorphic isoenzymes in grape cultivars

Loukas, M.; Stavrakakis, Manolis; Krimbas, C. B.

doi:10.1093/oxfordjournals.jhered.a109758

Cited by 26 publications

(15 citation statements)

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“…Thus, we jumped from the descriptive ampelography, which is still very useful for variety discrimination, to the ampelometry [6][7][8][9] and to the utilization of chemical and biochemical traits (i.e., the use of isozymes) [10][11][12][13][14]. Interestingly, it was noted that certain of such phenotypical traits could change under different environmental conditions [15,16].…”

Section: Introductionmentioning

confidence: 99%

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

et al. 2011

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The genetic grapevine intravarietal variability will be analyzed by PCR-derived marker systems. In particular, the object of the investigation will be the clonal variations of Malvasia nera di Brindisi/Lecce, Negroamaro and Primitivo, also known as Zinfandel, which are three grapevine varieties cultivated in Apulia region (Italy). In order to assess varietal identity of the samples, 132 DNA tests were performed by amplifying 16 SSR loci. The study of the intravarietal variability was performed using AFLPs, SAMPLs, ISSRs, and M-AFLPs. The application of the above-mentioned techniques allowed both to discriminate all genotypes of the three cultivars and to distinguish the accessions of each cultivar sampled from different geographic cultivation areas. Furthermore, the study of biotypes cultivated in different geographical environments of Salento (i.e., Apulia region) allowed important correlations between molecular marker variability and phenotypic traits. These results are suggesting both to focus our attention on the effects of the environment on the genotype and to consider, as a practical consequence, the importance of preserving autochthon grapevine biotypes found in different areas to truly preserve the richness of the germplasm. Thus, more accurate DNA studies give new information that can be extremely useful to the vine nurseries for the correct choice (i.e., supported by more accurate intravarietal variability analysis) of the grape multiplication materials.

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Section: Introductionmentioning

confidence: 99%

Study of Intra-Varietal Genetic Variability in Grapevine Cultivars by PCR-Derived Molecular Markers and Correlations with the Geographic Origins

et al. 2011

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“…However, these analyses are based on phenotypic characteristics which can be influenced and consequently complicated by environmental conditions [4][5][6][7][8][9][10][11]. Variability is particularly evident in widely distributed varieties that have been cultivated for centuries [1,7].…”

Section: Introductionmentioning

confidence: 99%

Inter- and Intra-Varietal Genetic Variability in Malvasia Cultivars

Meneghetti

Poljuha²,

Frare

et al. 2011

Mol Biotechnol

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The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia Aromatica; M. del Lazio; M. bianca lunga, also known as Malvasia del Chianti; M. nera di Brindisi/Lecce; M. di Casorzo; M. di Schierano, and M. nera di Bolzano) were analyzed using molecular approaches to study the genetic inter-varietal variability. Thirty Istrian Malvasia genotypes (i.e., 8 Italian clones, such as ISV 1, ISV F6, VCR 4, VCR 113, VCR 114, VCR 115, ERSA 120, ERSA 121, and 22 autochthonous grapevine accessions grown in Istrian Peninsula, Croatia) were investigated to evaluate the morphological and genetic intra-varietal variability. DNA analysis allowed discrimination of all Malvasia genotypes at molecular level using AFLP, SAMPL, and M-AFLP markers. Italian clones and autochthonous Croatian accessions of Istrian Malvasia were grouped according to their different geographic origins. These results showed the great genetic variability of Malvasia genotypes suggesting the need for the preservation of autochthonous grapevine biotypes found on different areas to approve the correct choice and selection of the grape multiplication materials.

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“…Isozymes, based on variability at proteins level [12], have frequently been used for these purposes [13][14][15] with different results depending on the genetic relationships among the materials analysed [16][17][18]. For these reasons, the DNA molecular analyses [19] with ampelography, ampelometry and chemistry, are essential for precise grapevine identification and also, to investigate the genetic differences among Vitis vinifera L. clones [20,21].…”

Section: Introductionmentioning

confidence: 99%