Inhibition of Aspergillus oryzae Mycelium Growth and Conidium Production by Irradiation with Light at Different Wavelengths and Intensities

Lin, Shangfei; Qin, Haiming; Zhang, Xiaolin; Li, Wenqi; Liu, Muqing

doi:10.1128/spectrum.00213-21

Cited by 7 publications

(7 citation statements)

References 31 publications

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“…CBL, whose intensity is higher than the threshold, significantly inhibited A. oryzae GDMCC 3.31. The threshold of blue light intensity for mycelium development and conidia generation were respectively 60 and 40 µmol•m −2 •s −1 [11]. Similar light responses have been reported in other A. oryzae strains [12].…”

Section: Introductionsupporting

confidence: 83%

“…A strain of Aspergillus oryzae that is safe to use and widely used in the brewing [1,2], enzyme [3,4], and waste treatment [5,6] industries is photoresponsive to different light wavelengths and intensities [7][8][9][10]. Our earlier investigation demonstrated that A. oryzae GDMCC 3.31 is more sensitive to CBL (475 nm) than to red light (630 nm) [11]. CBL, whose intensity is higher than the threshold, significantly inhibited A. oryzae GDMCC 3.31.…”

Section: Introductionmentioning

confidence: 93%

“…Although the peak light intensity of PBL was higher than that of CBL ( To investigate whether the different photoresponse was related to the threshold of light intensity (60 and 40 µmol•m −2 •s −1 ) established in our previous study [11], we generated five lower-level PBL with various peak light intensities and frequencies, and compared them to one CBL treatment with average light intensity of 20 µmol•m −2 •s −1 . As recorded in Table 2, the peak light intensities were ranged from 40 to 400 µmol•m −2 •s −1 , and frequency was maintained at 100 Hz.…”

Section: Light Conditions and Apparatusmentioning

confidence: 99%

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Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae

Lin

Jiang

et al. 2023

Fermentation

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Understanding how Aspergillus oryzae responds to light is critical for developing efficient light regulation strategies in the brewing and waste treatment industries. Although continuous light is known to restrict A. oryzae, little is known about A. oryzae’s sensitivity to light with photoperiod. In this study, we used pulse wave modulation (PWM) to generate nine pulsed blue light (PBL) treatments with varying peak light intensities and frequencies. The effect of PBL on A. oryzae was then compared to that of continuous blue light (CBL). Our findings showed that A. oryzae GDMCC 3.31 mycelium developed faster and produced more conidia under PBL with specific peak intensities and frequencies than under CBL treatment when the light dose and average light intensity were held constant. The colony diameter and conidia count under the two PBL treatments (PL-20_40%_1 Hz and PL-400_20%_10 kHz) were 1.13 and 1.22 times greater than under the CBL treatments, respectively. This different response may be mainly attributed to A. oryzae’s adaptation to the light–dark cycles in nature. Furthermore, an interactive effect was found between peak light intensity and frequency. This work includes pulsed wave modulation as a new factor that influences the A. oryzae photoresponse and recommends it in the development of light regulation methods for fermentation.

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Section: Introductionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 93%

Section: Light Conditions and Apparatusmentioning

confidence: 99%

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Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae

Lin

Jiang

et al. 2023

Fermentation

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“…These methods include colour filters with defined transmission spectra placed on the Petri dish lid [ 5 , 25 , 33 ], modified incubators with specific lamps or custom-made devices (e.g., [ 2 , 4 ]), and commercial growth cabinets with integrated illumination (e.g., [ 4 , 34 ]). Only one recent paper describes for the first time a targeted light incubation from below, thus enabling the LED light source to be replaced more easily [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Light in the box—photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

Vrabl

Zottele

Colleselli

et al. 2023

Fungal Biol Biotechnol

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Due to their versatile way of life as saprophytes, endophytes, and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M. brunneum (MA 43, formerly M. anisopliae var. anisopliae BIPESCO 5/F52) and B. brongniartii (BIPESCO 2). To achieve this, we constructed an easily adjustable illumination device for highly standardized photophysiological studies of fungi on Petri dishes, the so-called LIGHT BOX. With the aid of this device, M. brunneum and B. brongniartii were grown on S4G or S2G agar at 25 °C for 14 days either in complete darkness or under constant illumination with red light (λpeak = 635 nm), green light (λpeak = 519 nm) or blue light (λpeak = 452 nm). In addition, for each wavelength the effect of different illumination intensities was tested, i.e., intensities of red light ranging from 22.1 ± 0.1 to 136.5 ± 0.3 µW cm-2, green light from 16.5 ± 0.1 to 96.2 ± 0.1 µW cm-2, and blue light from 56.1 ± 0.2 to 188.9 ± 0.6 µW cm-2. Both fungi strongly responded in terms of growth, conidial production, pigmentation and morphology to changes in the wavelength and irradiation intensity. The wavelength-dependent production of the well-known secondary metabolite oosporein which is secreted by the genus Beauveria in particular, was also increased under green and blue light exposure. The established LIGHT BOX system allows not only to optimize conidial production yields with these biotechnologically relevant fungi, but also allows the photobiological exploration of other fungi.

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“…These methods include colour lters with de ned transmission spectra placed on the Petri dish lid [5,25,33], modi ed incubators with speci c lamps or custom-made devices (e.g., [2,4]), and commercial growth cabinets with integrated illumination (e.g., [4,34]). Only one recent paper describes for the rst time a targeted light incubation from below, thus enabling the LED light source to be replaced more easily [35].…”

Section: Introductionmentioning

confidence: 99%

Light in the box - photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

Vrabl

Zottele

Colleselli

et al. 2023

Preprint

View full text Add to dashboard Cite

Due to their versatile lifestyle as saprophytes, endophytes and entomopathogens, fungi of the genera Metarhizium and Beauveria are exposed to varying illumination conditions in their natural habitats, which makes a thorough adaptation to light very likely. While the few available studies for these genera support this assumption, research in this field is still in its infancy and the data material restricted to only a few fungal species. Thus, the aim of this work was to explore how light influences growth, conidial production and secondary metabolite formation of two industrial relevant strains of M. brunneum (MA 43, formerly M. anisopliae var. anisopliae BIPESCO 5/F52) and B. brogniartii (BIPESCO 2). To achieve this, we constructed an easily adjustable illumination device for highly standardized photophysiological studies of fungi on Petri dishes, the so-called LIGHT BOX. With the aid of this device, M. brunneum and B. brongniartii were grown on S4G or S2G agar at 25°C for 14 days either in complete darkness or under constant illumination with red light (λpeak = 635 nm), green light (λpeak = 519 nm) or blue light (λpeak = 452 nm). In addition, for each wavelength the effect of different illumination intensities was tested, i.e., intensities of red light ranging from 22.1 ± 0.1 to 136.5 ± 0.3 µW cm-², green light from 16.5 ± 0.1 to 96.2 ± 0.1 µW cm-², and blue light from 56.1 ± 0.2 to 188.9 ± 0.6 µW cm-². Both fungi strongly responded in terms of growth, conidial production, pigmentation and morphology to changes in the wavelength and irradiation intensity. The wavelength-dependent production of the well-known secondary metabolite oosporein which is secreted by the genus Beauveria in particular, was also increased under green and blue light exposure. The established LIGHT BOX system allows not only to optimize spore production yields with these biotechnologically relevant fungi, but also allows the photobiological exploration of other fungi.

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Inhibition of Aspergillus oryzae Mycelium Growth and Conidium Production by Irradiation with Light at Different Wavelengths and Intensities

Cited by 7 publications

References 31 publications

Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae

Effects of Pulsed Light on Mycelium Growth and Conidiation in Aspergillus oryzae

Light in the box—photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

Light in the box - photobiological examination chamber with light trap ventilation system for studying fungal surface cultures illustrated with Metarhizium brunneum and Beauveria brongniartii

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