Inhibition of Quorum Sensing in
            <i>Serratia marcescens</i>
            AS-1 by Synthetic Analogs of
            <i>N</i>
            -Acylhomoserine Lactone

Morohoshi, Toshio; Shiono, Toshitaka; Takidouchi, Kiyomi; Kato, Masashi; Kato, Norihiro; Kato, Junichi; Ikeda, Tsukasa

doi:10.1128/aem.00593-07

Cited by 148 publications

(135 citation statements)

References 31 publications

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“…The A. hydrophila SSU WT strain, its various isogenic mutants and their corresponding complemented strains were grown directly in 3 ml LB medium contained in polystyrene tubes at 37 uC for 24 h with shaking. Biofilm formation was quantified according to a procedure described elsewhere (Morohoshi et al, 2007). Finally, biofilm formation results were normalized to 1610 9 c.f.u.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis

et al. 2013

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Aeromonas hydrophila, a Gram-negative bacterium, is an emerging human pathogen equipped with both a type 3 and a type 6 secretion system (T6SS). In this study, we evaluated the roles played by paralogous T6SS effector proteins, hemolysin co-regulated proteins (Hcp-1 and -2) and valine glycine repeat G (VgrG-1, -2 and -3) protein family members in A. hydrophila SSU pathogenesis by generating various combinations of deletion mutants of the their genes. In addition to their predicted roles as structural components and effector proteins of the T6SS, our data clearly demonstrated that paralogues of Hcp and VgrG also influenced bacterial motility, protease production and biofilm formation. Surprisingly, there was limited to no observed functional redundancy among and/or between the aforementioned T6SS effector paralogues in multiple assays. Our data indicated that Hcp and VgrG paralogues located within the T6SS cluster were more involved in forming T6SS structures, while the primary roles of Hcp-1 and VgrG-1, located outside of the T6SS cluster, were as T6SS effectors. In terms of influence on bacterial physiology, Hcp-1, but not Hcp-2, influenced bacterial motility and protease production, and in its absence, increases in both of the aforementioned activities were observed. Likewise, VgrG-1 played a major role in regulating bacterial protease production, while VgrG-2 and VgrG-3 were critical in regulating bacterial motility and biofilm formation. In an intraperitoneal murine model of infection, all Hcp and VgrG paralogues were required for optimal bacterial virulence and dissemination to mouse peripheral organs. Importantly, the observed phenotypic alterations of the T6SS mutants could be fully complemented. Taking these results together, we have further established the roles played by the two known T6SS effectors of A. hydrophila by defining their contributions to T6SS function and virulence in both in vitro and in vivo models of infection.

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Section: Methodsmentioning

confidence: 99%

Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis

et al. 2013

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“…Such high-throughput screening has also led to the identification of two classes of molecules able to suppress V. cholerae virulence factor expression through modulation of either the noncanonical AHL receptor LuxN or the NtrC-like response regulator LuxO (189). Additionally, structure-based derivatiza-tion of previously identified antagonists has led to the discovery of multiple non-AHL pharmacophores with activity against LuxRtype proteins, including disubstituted imidazolium salts (183,190) and N-acyl cyclopentylamides (191,192).…”

Section: Inhibition Of Signal Detection Synthetic Signal Analogues Anmentioning

confidence: 99%

“…However, there are some data addressing these issues. Various compounds have been demonstrated to inhibit in vitro behaviors, including but not limited to P. aeruginosa biofilm formation (118,126,168,200,203), Serratia marcascens swarming and biofilm formation (192), and carbapenem production in Serratia spp. (186).…”

Section: Inhibition Of Signal Detection Synthetic Signal Analogues Anmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

689

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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“…Little is known about the antimicrobial effects of the fruit and no study has been found reporting its anti-quorum sensing activity. Therefore, this work aimed at evaluating the quorum sensing inhibitory potential of phenolic extracts obtained from E. brasiliensis fruit against commonly phenotypes regulated by quorum sensing in Chromobacterium violaceum, Aeromonas hydrophila and Serratia marcescens (Morohoshi et al, 2007;Pinto et al, 2007;Ponce-Rossi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

et al. 2016

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The aim of this study was to assess the anti-quorum sensing activity of phenolic extracts from grumixama (Eugenia brasiliensis), also known as Brazilian cherry, in concentrations that did not interfere with bacterial growth. The pulp phenolic compounds were extracted by using solid phage extraction in a mini-collumn C18 and quantified by spectrophotometry. The anti-quorum sensing activity was evaluated by testing the inhibition of violacein production in Chromobacterium violaceum and by evaluating the swarming motility in Aeromonas hydrophila and Serratia marcescens, both phenotypes regulated by quorum sensing. The phenolic extract strongly inhibited the production of violacein in C. violaceum, reducing its production in comparison with a control with no extract. No inhibition of growth was observed at the concentrations tested for quorum sensing inhibition. Confirming the quorum sensing inhibition phenotype, the extract was also able to inhibit swarming motility in S. marcescens and in A. hydrophila, although in the later the effect was marginal. Dverall, these results indicate that phenolic extract from E. brasiliensis presents quorum sensing inhibitory activity most likely due to the presence of fruit phenolics which have been implicated as quorum sensing inhibitors in Gram negative bacteria.Keywords: quorum sensing; anti-quorum sensing; phenolic extract; Brazilian cherry; Eugenia brasiliensis. Practical Application:The phenolic extract in this study presents potential applications in the food and pharmaceutical industries due to inhibition of phenotypes regulated by quorum sensing, which include production of virulence factors and spoilage enzymes. By inhibiting phenotypes associated with quorum sensing, the extract presents potential application as a natural preservative for food conservation and as an alternative or an adjuvant with antibiotics.

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Inhibition of Quorum Sensing in Serratia marcescens AS-1 by Synthetic Analogs of N -Acylhomoserine Lactone

Cited by 148 publications

References 31 publications

Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis

Evaluation of the roles played by Hcp and VgrG type 6 secretion system effectors in Aeromonas hydrophila SSU pathogenesis

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

Anti-quorum sensing activity of phenolic extract from Eugenia brasiliensis (Brazilian cherry)

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