Inhibition of the mitochondrial ATP synthesis by polygodial, a naturally occurring dialdehyde unsaturated sesquiterpene

Castelli, María Victoria; Lodeyro, Anabella F.; Malheiros, Ângela; Zacchino, Susana; Roveri, Oscar A.

doi:10.1016/j.bcp.2005.04.016

Cited by 39 publications

(35 citation statements)

References 35 publications

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“…Dill oil inhibited an isolated mitochondrial ATPase in a dose-dependent manner at different concentrations, which manifests the idea of mitochondrial ATPase being a physiologically relevant target of dill oil in A. flavus . Similar types of results were also reported by Lunde et al [44] and Castelli et al [42].…”

Section: Discussionsupporting

confidence: 90%

The Mechanism of Antifungal Action of Essential Oil from Dill (Anethum graveolens L.) on Aspergillus flavus

et al. 2012

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The essential oil extracted from the seeds of dill (Anethum graveolens L.) was demonstrated in this study as a potential source of an eco-friendly antifungal agent. To elucidate the mechanism of the antifungal action further, the effect of the essential oil on the plasma membrane and mitochondria of Aspergillus flavus was investigated. The lesion in the plasma membrane was detected through flow cytometry and further verified through the inhibition of ergosterol synthesis. The essential oil caused morphological changes in the cells of A. flavus and a reduction in the ergosterol quantity. Moreover, mitochondrial membrane potential (MMP), acidification of external medium, and mitochondrial ATPase and dehydrogenase activities were detected. The reactive oxygen species (ROS) accumulation was also examined through fluorometric assay. Exposure to dill oil resulted in an elevation of MMP, and in the suppression of the glucose-induced decrease in external pH at 4 µl/ml. Decreased ATPase and dehydrogenase activities in A. flavus cells were also observed in a dose-dependent manner. The above dysfunctions of the mitochondria caused ROS accumulation in A. flavus. A reduction in cell viability was prevented through the addition of L-cysteine, which indicates that ROS is an important mediator of the antifungal action of dill oil. In summary, the antifungal activity of dill oil results from its ability to disrupt the permeability barrier of the plasma membrane and from the mitochondrial dysfunction-induced ROS accumulation in A. flavus.

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Section: Discussionsupporting

confidence: 90%

The Mechanism of Antifungal Action of Essential Oil from Dill (Anethum graveolens L.) on Aspergillus flavus

et al. 2012

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“…The surface potential (Gouy-Chapman, s ) contributestogether with the membrane dipole potential ( d ) and the transmembrane potentials ( ) -to the potential difference between the membrane surfaces ( ϕ) and determines the electrical potential profile in the membrane [48]. Therefore, the change of the surface potential by MDpep5 would affect the potential profile in the membrane and the interfacial membrane potential difference ( ϕ).…”

Section: Effect Of Mdpep5 On the Bacterial Electrostatic Surface Potementioning

confidence: 98%

Insertion mode of a novel anionic antimicrobial peptide MDpep5 (Val-Glu-Ser-Trp-Val) from Chinese traditional edible larvae of housefly and its effect on surface potential of bacterial membrane

Tang

Shi

Zhao

et al. 2008

Journal of Pharmaceutical and Biomedical Analysis

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“…Polygodial was identified by comparison of its retention time (17.62 min) and the MS spectrum with an authentic sample obtained from our previous works (Castelli et al, 2005;Malheiros et al, 2005;Derita et al, 2008). The linearity of the detector response was verified using a series of two-fold diluted DCM solutions of polygodial.…”

Section: Quantification Of Polygodial In Extracts From L S and F Colmentioning

confidence: 99%

“…Repeated column chromatographies of fractions 5-8 led to the isolation of the sesquiterpenes polygodial (1) (1925 mg), isopolygodial (2) (70 mg), drimenol (3) (30 mg) and confertifolin (4) (35 mg). Pure compounds were identified by micromelting point, optical rotation and spectroscopic data including 1 H and 13 CNMR (Ying et al, 1995;Barnes and Loder, 1962;Guillerm et al, 1984) and were compared with authentic samples obtained previously in our laboratory (Castelli et al, 2005;Malheiros et al, 2005;Derita et al, 2008 for polygodial and isopolygodial) or with literature data (Rodríguez et al, 2005;Urban and Capon, 1996, for drimenol;Hueso-Rodríguez and Rodríguez, 1989;Appel et al, 1963, for confertifolin).…”

Section: Bioassay-guided Fractionation Of Adcmmentioning

confidence: 99%

Influence of plant part, season of collection and content of the main active constituent, on the antifungal properties of Polygonum acuminatum Kunth.

Derita

Leiva

Zacchino

2009

Journal of Ethnopharmacology

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Inhibition of the mitochondrial ATP synthesis by polygodial, a naturally occurring dialdehyde unsaturated sesquiterpene

Cited by 39 publications

References 35 publications

The Mechanism of Antifungal Action of Essential Oil from Dill (Anethum graveolens L.) on Aspergillus flavus

The Mechanism of Antifungal Action of Essential Oil from Dill (Anethum graveolens L.) on Aspergillus flavus

Insertion mode of a novel anionic antimicrobial peptide MDpep5 (Val-Glu-Ser-Trp-Val) from Chinese traditional edible larvae of housefly and its effect on surface potential of bacterial membrane

Influence of plant part, season of collection and content of the main active constituent, on the antifungal properties of Polygonum acuminatum Kunth.

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