Inhibitory Activity of Cobalt(ii)–morin Complex Against the Replication of Dengue Virus Type 2

Sucipto, Teguh Hari; Churrotin, Siti; Setyawati, Harsasi; Mulyatno, Kris Cahyo; Amarullah, Ilham Harlan; Ueda, Shuhai; Kotaki, Tomohiro; Sumarsih, Sri; Wardhani, Puspa; Bendryman, Sri Subekti; Aryati, Aryati; Soegijanto, Soegeng; Kameoka, Masanori

doi:10.20473/ijtid.v6i6.6126

Cited by 7 publications

(6 citation statements)

References 20 publications

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“…The IC 50 value of the Cobalt(II)-Morin complex for DENV-2 was 3.08 μg/ml. 25 MB21, a benzimidazole derivative, was found to be the most potential inhibitor of cloned proteases (IC 50 = 5.95 μM). 26 This study suggest that of Zinc(II)-2,4,5triphenyl-1H-imidazole complex compound can't be an attractive antiviral option.…”

Section: Resultsmentioning

confidence: 99%

Eﬀect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Sucipto¹,

Wibrianto

Martak

et al. 2020

IJTID

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Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. DENVs are transmitted to humans through female mosquitoes from Aedes aegypti and Aedes albopictus species. Indonesia is one of the largest countries in the world in dengue endemic regions worldwide. Dengue fever was occurred for the first time as an outbreak in Surabaya and Jakarta in 1968. Many eﬀorts have been made to prevent and treat DENV infections, and clinical trials of a number of vaccines are currently underway. Antiviral testing of DENV is an important alternative for drug characterization and development. Complex compounds are formed as a result of metal and organic complex reactions. Complex compounds can be used as an anti-inflammatory, antimicrobial antifungal, antibacterial, antivirus. The Zn2+ ion can be used as an antiviral candidate. The purpose of this project was investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole antiviral compound to be further tested for inhibitory eﬀect on the replication of DENV-2 in cell culture. DENV replication was measured by antiviral activity assay and cytotoxicity assay. The inhibitory activity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by Viral ToxGloTM Assay. The cytotoxicity of Zinc(II)-2,4,5-triphenyl-1H-imidazole complex compound was determined by CellTiter96® AQuoeus assay. The inhibitory concentration (IC50) of Zinc(II)-2,4,5-triphenyl- 1H-imidazole against dengue virus type-2 was 34.42 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was <100 μg/ml. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of investigated Zinc(II)-2,4,5-triphenyl-1H-imidazole complex and its high toxicity in Vero cells. Further studies are not required before investigated Zinc(II)-2,4,5-triphenylimidazole can be applied in the treatment of DENV-2 infections

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Section: Resultsmentioning

confidence: 99%

Eﬀect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Sucipto¹,

Wibrianto

Martak

et al. 2020

IJTID

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“…Previous reports have described the use of cobalt(II)-morin and zinc(II)-morin based systems for anti-DENV (dengue serotype) type 2 applications. The value of inhibition activity of zinc(II)-morin was 2.00 µg/ml and cobalt(II)morin was 3.08 µg/ml (Sucipto et al, 2017;Sucipto et al, 2019).…”

Section: Introductionmentioning

confidence: 93%

“…Until now, there is no vaccine or anti-viral for humans that could overcome this disease. Imidazole and their derivatives are proven has potency against dengue virus infection in cell lines (Sucipto et al, 2018;Sucipto et al, 2017). On the other hand, the presence of metal on the structure of organic ligand is proven could improve their biological activity (Geersing et al, 2018) The complex compound is formed as a result of metal and organic compound reaction.…”

Section: Introductionmentioning

confidence: 99%

The influence of metal on the performance of 2,4,5-triphenylimidazole as an inhibitor of dengue virus replication

Sumarsih

Pratiwi

Ainni

et al. 2020

APJMBB

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Dengue is a contagious disease caused by dengue virus and transmitted to humans by the bite of infected Aedes aegypti mosquito. Imidazole and its derivatives are proven have ability impairing dengue virus. One of potential imodazole’s derivatives is 2,4,5-triphenylimidazole (TPI). The presence of metal to the 2,4,5-triphenylimidazole (TPI) structure through a complex compound formation highly contributes to their ability as an inhibtor dengue virus replication. Iron, cobalt and zinc were used as an ion center in the complex compound. Complex Zn-TPI and Fe-TPI showed low cytotoxic effect at all the evaluated concentrations (viability > 50%). Complex Co-TPI showed reduction of DENV-3 growth, at the lowest concentration (6.25 µg/ml) exhibited the antiviral activity (DENV-3 reduction 43%). For Fe-TPI and Zn-TPI, the reduction values of DENV-3 were 56% and 54.9% respectively.

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“…Previous reports have described the use of cobalt(II)-morin and zinc(II)-morin based systems for anti-DENV type 2 applications. The value of activity inhibition of zinc(II)-morin was 2.00 µg/mL and cobalt(II)-morin was 3.08 µg/mL 11,12 .…”

Section: Introductionmentioning

confidence: 94%

Inhibition of Dengue Virus Serotype 2 in Vero Cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2

Sucipto

Martak

2020

Infectious Disease Reports

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Dengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or antiviral chemotherapeutics are available for humans, and disease treatment is limited to supportive care. Over the years there has been a continuous interest in the chemistry of metal complexes with biological activity, including platinum complexes with antitumor activity and silver complexes with antimicrobial action. Aim of the project was to investigate [Cu(2,4,5-triphenyl-1H- imidazole)2 (H2O)2].Cl2 as antiviral compound that was further tested for inhibitory effect on the replication of dengue virus type 2 (DENV-2) in Vero cell. DENV-2 were infected in Vero cells and replication of virus was measured by Viral ToxGlo with selectivity index value (SI) and determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for com- pound. The standard curve between concentration of compound (μg/mL) and %viability of cells was analyzed by logarithmic cor- relation regression with regression equation. For infection rates, t-test was used to exam- ine the statistical significances among the concentrations of compound. P<0.05 was considered to be significant. The maximum inhibitory concentration (IC50) of [Cu(2,4,5- triphenyl-1H-imidazole)2 (H2O)2].Cl2 against DENV-2 was 98.62 μg/mL. The cytotoxic concentration (CC50) of compound against Vero cells was 300.36μg/mL. The SI values for [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 1.86. Based on selectivity index values, [Cu(2,4,5-triphenyl-1H-imidazole)2 (H2O)2].Cl2 can inhibit the growth of DENV- 2 and has low toxicity values for Vero cells.

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Inhibitory Activity of Cobalt(ii)–morin Complex Against the Replication of Dengue Virus Type 2

Cited by 7 publications

References 20 publications

Eﬀect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

Eﬀect of Zinc(II)-2,4,5-triphenyl-1H-imidazole Complex Against Replication DENV-2 in Vero Cell

The influence of metal on the performance of 2,4,5-triphenylimidazole as an inhibitor of dengue virus replication

Inhibition of Dengue Virus Serotype 2 in Vero Cells with [Cu(2,4,5-triphenyl-1H-imidazole)2(H2O)2].Cl2

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