Inhibitory effect of GA3 on the development of thidiazuron-induced somatic embryogenesis in geranium (Pelargonium xhortorum Bailey) hypocotyl cultures

Hutchinson, M. J.; KrishnaRaj, Sankaran; Saxena, Praveen K.

doi:10.1007/bf01146789

Cited by 22 publications

(16 citation statements)

References 20 publications

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“…GAs have been reported to have effects on somatic embryo development in contexts other than pkl and outside of the meristem (for example, Hutchinson et al, 1997;Ondrej et al, 2002;Chen and Chang, 2003;Tokuji and Kuriyama, 2003). In carrot (Daucus carota) hypocotyl explants, GA inhibits early stages of somatic embryogenesis, whereas uniconazole, an inhibitor of GA biosynthesis, promotes secondary embryogenesis from primary embryos (Tokuji and Kuriyama, 2003).…”

Section: Discussionmentioning

confidence: 99%

The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

et al. 2004

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AGL15 (for AGAMOUS-Like 15) is a member of the MADS domain family of DNA binding transcriptional regulators that accumulates to its highest amounts during embryo development. To better understand how AGL15 functions, a chromatin immunoprecipitation approach was used to identify directly regulated genes. One DNA fragment that coprecipitated with AGL15 corresponded to a portion of the regulatory region of a gene named DTA1 (for Downstream Target of AGL15-1). The expression of DTA1 was positively correlated with AGL15 abundance during embryogenesis. In this report, a cis element for response to AGL15 was identified, and the activity of DTA1 as a gibberellin (GA) 2-oxidase was confirmed. DTA1 corresponds to AtGA2ox6 and was renamed to indicate this identity. Further experiments related the function of AtGA2ox6 to regulation by AGL15. Constitutive expression of AGL15 and of AtGA2ox6 altered endogenous GA amounts and caused GA-deficient phenotypes in Arabidopsis thaliana that could be at least partially rescued by application of biologically active GA. The phenotype of plants with decreased expression of AtGA2ox6 was the converse of plants overexpressing AtGA2ox6 in terms of seed germination attributes and effects on somatic embryo production.

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Section: Discussionmentioning

confidence: 99%

The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

et al. 2004

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“…Exposure of mung bean hypocotyls to TDZ responded in ethylene production and at the same time storage of auxin and Ca 2+ occurred (Yip and Yang, 1986). A rise up in ethylene level was investigated during the somatic embryogenesis of geranium via TDZ in the upper space of culture vessel (Hutchinson et al, 1997). Embryogenic response in geranium hypocotyls was enhanced by incorporating ethylene inhibitor AVG (amino ethoxyvinylglycine), which is a sign for the proof that ethylene is a harmful by-product of TDZ induced metabolic process (Hutchinson et al, 1997).…”

Section: Involvement Of Phytohormonesmentioning

confidence: 99%

“…A rise up in ethylene level was investigated during the somatic embryogenesis of geranium via TDZ in the upper space of culture vessel (Hutchinson et al, 1997). Embryogenic response in geranium hypocotyls was enhanced by incorporating ethylene inhibitor AVG (amino ethoxyvinylglycine), which is a sign for the proof that ethylene is a harmful by-product of TDZ induced metabolic process (Hutchinson et al, 1997). However, the embryogenic response is decreased to the same extent as resulted in TDZ stimulated culture by the usage of exogenous ethylene or an ethylene precursor that is, 1-aminocyclopropane-1-carboxylic acid.…”

Section: Involvement Of Phytohormonesmentioning

confidence: 99%

Thidiazuron: A multi-dimensional plant growth regulator

Guo¹,

Abbasi²,

Zeb³

et al. 2011

Afr. J. Biotechnol.

200

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Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZapplication in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological (physiological and biochemical) events in cells are induced or enhanced by TDZ, but the mode of action of TDZ is yet unknown. However, varieties of underlying mechanisms were revealed by reports showing how morphogenic events were induced by application of TDZ. Other reports showed that TDZ may modify endogenous plant growth regulators, either directly or indirectly and produce reactions in cell/tissue, necessary for its division/regeneration. Other possibilities include modification in cell membrane, energy levels, nutrient absorption, transport and assimilation, etc. In this review, recent advancements in TDZ application in plant sciences are discussed.

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“…Especially, the involvement of gibberellin (GA) is not conclusive, because its eŠects vary in diŠerent tissues and species. For example, exogenously applied GA3 stimulated embryogenesis in immature cotyledon cultures from chickpea, 4) geranium hypocotyls cultures, 5) and Medicago sativa tissue cultures. 6) In the geranium and Medicago cultures, GA biosytnthesis inhibitors such as paclobutrazol inhibited embryo production.…”

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confidence: 99%

Gibberellin Is Essentially Required for Carrot (Daucus carotaL.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions

Mitsuhashi

Toyomasu

Masui

et al. 2003

Bioscience, Biotechnology, and Biochemistry

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Inhibitory effect of GA3 on the development of thidiazuron-induced somatic embryogenesis in geranium (Pelargonium xhortorum Bailey) hypocotyl cultures

Cited by 22 publications

References 20 publications

The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

Thidiazuron: A multi-dimensional plant growth regulator

Gibberellin Is Essentially Required for Carrot (Daucus carotaL.) Somatic Embryogenesis: Dynamic Regulation of Gibberellin 3-Oxidase Gene Expressions

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