Inhibitory Effect of Wheat Germ Agglutinin on Mouse Mast Cell Adhesion to Fibronectin

Abstract

Background: Mast cells play a critical role in allergic and inflammatory responses. The interactions between these cells and extracellular matrix components influence the distribution of mast cells in tissues and their biological responsiveness. It has been reported that the lectin wheat germ agglutinin (WGA) inhibits mast cell mediator release. We decided to investigate whether adhesion to fibronectin (FN), another mast cell function, which is upregulated following FcεRI cross-linking, is also inhibited by WGA. Methods: Mouse bone-marrow-derived mast cell line MCP5/L was used. For FcεRI-dependent mast cell activation, MCP5/L cells were sensitized with mouse IgE antibodies. WGA was added to cell suspensions simultaneously with a challenging agent and, after an appropriate incubation period, β-hexosaminidase release and adhesion to FN were determined. Results: Both FcεRI cross-linking-dependent mast cell adhesion to FN and mediator release were dose-dependently inhibited by WGA; however, the lectin concentrations required to induce maximum inhibition of adhesion were significantly lower. Furthermore, WGA inhibited phorbol-myristate-acetate- and A-23187-mediated mast cell adhesion to FN, i.e. processes that do not engage FcεRI. The effect of WGA on FcεRI-mediated secretion was reversed by GlcNAc. In contrast, combination of GlcNAc and NeuNAc or N,N′-diacetylchitobiose was required to reverse the inhibitory effect of WGA on mast cell adhesion. Conclusion: The characteristics of WGA-mediated inhibition of MCP5/L mast cell adhesion to FN suggest that mast cell integrins are targets of the inhibitory action of WGA and the sugar moieties on these receptors might be important for receptor function.