Inhibitory effects and gene expression analysis of chemotherapeutic photodynamic therapy by using a liposomally formulated indocyanine green derivative

Okamoto, Yoshiharu; Ishizuka, Mariko; Sumiyama, Fusao; Kosaka, Hisashi; Suganami, Akiko; Tsutsumi, Yutaka; Sekimoto, Mitsugu; Kaibori, Masaki

doi:10.1016/j.pdpdt.2022.102961

Cited by 4 publications

(4 citation statements)

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“…ICG-Lipo provides an excellent drug delivery system and has the potential to provide antitumor effects with the minimum amount of drug required. Previously, we succeeded in reducing the amount of drug contained in ICG-Lipo to about 10% of the normal dose in companion animals ( 21 ). To reduce side effects and to take advantage of the fact that PDT does not cause cumulative toxicity, it is important to minimize the amount of drug in ICG-Lipo.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, a recent study in the field of photoimmunotherapy described the development of an approach in which NIR-PIT targeted EGFR and human EGFR2 expressed in tumor tissues as well as cytotoxic T cells and regulatory T cells ( 9 ). Moreover, an approach in which multiple drugs with different mechanisms of action were encapsulated inside ICG-Lipo was shown to produce more potent antitumor effects ( 21 ). In the future, it may be possible to develop ICG-Lipo to simultaneously encapsulate multiple anticancer drugs, antihormonal drugs, and molecular-targeted drugs.…”

Section: Discussionmentioning

confidence: 99%

“…shown to produce more potent antitumor effects (21). In the future, it may be possible to develop ICG-Lipo to simultaneously encapsulate multiple anticancer drugs, antihormonal drugs, and moleculartargeted drugs.…”

Section: Discussionmentioning

confidence: 99%

“…The antitumor effect of ICG-Lipo against spontaneous tumors in animals has already been reported ( 19 , 20 ). ICG-Lipo can also be used as a carrier for multiple drugs ( 21 ). We have developed ICG-Lipo-PTX, in which PTX is encapsulated inside ICG-Lipo ( Figure 1 ).…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic therapy with paclitaxel-encapsulated indocyanine green-modified liposomes for breast cancer

Ishizuka,

Kaibori,

Sumiyama

et al. 2024

Front. Oncol.

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BackgroundPhotodynamic therapy (PDT) involves the administration of a photosensitizing agent and irradiation of light at an excitation wavelength that damages tumor cells without causing significant damage to normal tissue. We developed indocyanine green (ICG)-modified liposomes in which paclitaxel (PTX) was encapsulated (ICG-Lipo-PTX). ICG-Lipo-PTX accumulates specifically in tumors due to the characteristics of the liposomes. The thermal and photodynamic effects of ICG and the local release of PTX by irradiation are expected to induce not only antitumor effects but also cancer immunity. In this study, we investigated the antitumor effects of ICG-Lipo-PTX in breast cancer.MethodsThe antitumor effects of ICG-Lipo-PTX were examined in xenograft model mice subcutaneously implanted with KPL-1 human breast cancer cells. ICG-Lipo-PTX, ICG-Lipo, or saline was administered intraperitoneally, and the fluorescence intensity was measured with a fluorescence imaging system (IVIS). Intratumor temperature, tumor volume, and necrotic area of tumor tissue were also compared. Next, we investigated the induction of cancer immunity in an allogeneic transplantation model in which BALB-MC mouse breast cancer cells were transplanted subcutaneously in the bilateral inguinal region. ICG-Lipo-PTX was administered intraperitoneally, and PDT was performed on only one side. The fluorescence intensity measured by IVIS and the bilateral tumor volumes were compared. Cytokine secretory capacity was also evaluated by ELISPOT assay using splenocytes.ResultsIn the xenograft model, the fluorescence intensity and temperature during PDT were significantly higher with ICG-Lipo-PTX and ICG-Lipo in tumor areas than in nontumor areas. The fluorescence intensity in the tumor area was reduced to the same level as that in the nonirradiated area after two times of irradiation. Tumor growth was significantly reduced and the percentage of necrotic area in the tumor was higher after PDT in the ICG-Lipo-PTX group than in the other groups. In the allograft model, tumor growth on day 14 in the ICG-Lipo-PTX group was significantly suppressed not only on the PDT side but also on the non-PDT side. In addition, the secretion of interferon-γ and interleukin-2 was enhanced, whereas that of interleukin-10 was suppressed, in the ICG-Lipo-PTX group.ConclusionThe PDT therapy with ICG-Lipo-PTX may be an effective treatment for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%