Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis

Chotigarpa, Rinrada; Lampang, Kannika Na; Pikulkaew, Surachai; Okonogi, Siriporn; Ajariyakhajorn, Kittisak; Mektrirat, Raktham

doi:10.3390/scipharm86030029

Cited by 11 publications

(8 citation statements)

References 32 publications

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“…Human sweat contained approximately 0.1% lactate [32]. In this study, we utilized the 0.02% sodium l -lactate for S. epidermidis fermentation (Figure 1) since the higher sodium l -lactate may exert a toxic effect on the bacterial growth [33]. Lactate is fermented to acetate and propionate by various bacteria including Propionibacterium spp.…”

Section: Discussionmentioning

confidence: 99%

5-Methyl Furfural Reduces the Production of Malodors by Inhibiting Sodium l-Lactate Fermentation of Staphylococcus epidermidis: Implication for Deodorants Targeting the Fermenting Skin Microbiome

et al. 2019

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Staphylococcus epidermidis (S. epidermidis) is a common bacterial colonizer on the surface of human skin. Lactate is a natural constituent of skin. Here, we reveal that S. epidermidis used sodium l-lactate as a carbon source to undergo fermentation and yield malodors detected by gas colorimetric tubes. Several furan compounds such as furfural originating from the fermentation metabolites play a role in the negative feedback regulation of the fermentation process. The 5-methyl furfural (5MF), a furfural analog, was selected as an inhibitor of sodium l-lactate fermentation of S. epidermidis via inhibition of acetolactate synthase (ALS). S. epidermidis treated with 5MF lost its ability to produce malodors, demonstrating the feasibility of using 5MF as an ingredient in deodorants targeting malodor-causing bacteria in the skin microbiome.

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Section: Discussionmentioning

confidence: 99%

5-Methyl Furfural Reduces the Production of Malodors by Inhibiting Sodium l-Lactate Fermentation of Staphylococcus epidermidis: Implication for Deodorants Targeting the Fermenting Skin Microbiome

et al. 2019

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“…In addition, LA antimicrobial action is known to be strongly dependent on the concentration of the acid and on the pH under which the experiment is carried out [70]. Some authors [16,25] demonstrated that under low pH, the biocide permeabilizes the outer membrane of Gram-negative bacteria. It was suggested that LA acts as a protonator of anionic components like the carbonyl and phosphate groups and consequently, the molecular interactions between components within the membrane are weakened [25,71].…”

Section: Discussionmentioning

confidence: 99%

“…Organic acids and quaternary ammonium compounds (QACs) are among the different classes of biocides that are widely used [16,17,18,19,20,21]. Organic acids are a class of disinfectants particularly used in the food industry [9,22,23,24].…”

Section: Introductionmentioning

confidence: 99%

Biocide Potentiation Using Cinnamic Phytochemicals and Derivatives

et al. 2019

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Surface disinfection is of utmost importance in the prevention of bacterial infections. This study aims to assess the ability of ten phytochemicals and related derivatives as potentiators of two commonly used biocides—cetyltrimethylammonium bromide (CTAB) and lactic acid (LA). LA in combination with cinnamic, hydrocinnamic, α-methylcinnamic, and α-fluorocinnamic acids had a factional inhibitory concentration index (FICI) ≤ 1 for Escherichia coli and Staphylococcus aureus. Several phytochemicals/derivatives in combination with biocides improved the biocidal efficacy against early sessile bacteria. The most effective combination was LA with allyl cinnamate (2.98 ± 0.76 log CFU·cm−2 reduction) against E. coli. The combination with CTAB was successful for most phytochemicals/derivatives with a maximum bactericidal efficacy against sessile E. coli when combined with allyl cinnamate (2.20 ± 0.07 log CFU·cm−2 reduction) and for S. aureus when combined with α-methylcinnamic acid (1.68 ± 0.30 log CFU·cm−2 reduction). This study highlights the potential of phytochemicals and their derivatives to be used in biocide formulations.

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“…[21] and Chotigarpa et al . [22]. Briefly, the modified rice powder was used for gel base preparation and viscosity building agent.…”

Section: Methodsmentioning

confidence: 99%

Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

et al. 2019

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Aim: This study aimed to estimate the enumeration of total bacteria and coliform on teat skin from dairy cows and evaluate the efficacy of the natural rice gel containing 5% v/v lactic acid (NGL) against Escherichia coli standard and field strains isolated from bovine teat skin. Materials and Methods: A total of 100 bacterial teat skin samples (25 cows) were collected from dairy cows in smallholder farm. The cows were housed in freestall barns. The colonization of total bacteria and E. coli on teat skin was measured by 3M Petrifilm method. The minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of lactic acid were evaluated for reference strain of E. coli ATCC 25922 and two field strains of E. coli. The natural teat sanitizer was formulated using 5% NGL with modified rice gel. In vitro antiseptic efficacy of 5% NGL was determined by time-kill kinetic assay. E. coli morphology after exposure with 5% NGL was examined under a scanning electron microscope (SEM). Results: The total bacteria and coliform counts from bovine teat skin were 2.11×104 and 1.54×101 colony-forming units/ml, respectively. The MIC and MBC of lactic acid on the tested bacteria were 0.5% v/v. The natural teat dip was successfully prepared with minimum change in consistency after 1 year of storage at 4°C. The reduction rate of 5% NGL on E. coli ATCC 25922 and field strain showed 32.77% and 27.58%, respectively. An appearance under SEM of non-viable E. coli after being incubated with 5% NGL clearly showed atypical form and rough surface cell membrane. Conclusion: The rice gel containing 5% v/v lactic acid is a promising preparation as a natural teat antiseptic for reducing bacteria on teat skin. It was shown to be effective against E. coli causing bovine mastitis in dairy cows.

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Inhibitory Effects and Killing Kinetics of Lactic Acid Rice Gel Against Pathogenic Bacteria Causing Bovine Mastitis

Cited by 11 publications

References 32 publications

5-Methyl Furfural Reduces the Production of Malodors by Inhibiting Sodium l-Lactate Fermentation of Staphylococcus epidermidis: Implication for Deodorants Targeting the Fermenting Skin Microbiome

5-Methyl Furfural Reduces the Production of Malodors by Inhibiting Sodium l-Lactate Fermentation of Staphylococcus epidermidis: Implication for Deodorants Targeting the Fermenting Skin Microbiome

Biocide Potentiation Using Cinnamic Phytochemicals and Derivatives

Antiseptic effect of natural teat dip containing lactic acid against mastitis-causing Escherichia coli

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