Inhibitory Effects of Agaricus blazei on Mast Cell-Mediated Anaphylaxis-Like Reactions

Abstract

Agaricus blazei is a medically important mushroom widely eaten and prescribed around the world. It is belonged to the Agaricaceae family and is popularly known as "Himematsutake" in Japan. In Brazil, this mushroom is used as a traditional medicine for the prevention of cancer, diabetes, hyperlipidemia, arteriosclerosis, and chronic hepatitis. Recently, it has been reported that Agaricus blazei has the anti-tumor, immunomodulatory, anti-mutagenic, anti-oxidant, and anti-bacterial activities.1-6) However, it has not been cleared whether it prevents allergic diseases in experimental model.In general, immediate-type hypersensitivity reactions that involve asthma, allergic rhinitis, urticaria, and anaphylaxis are mediated by mast cells. In response to the IgE-dependent and IgE-independent stimuli, mast cells elicit degranulation, which results in releasing various mediators, such as histamine and an array of inflammatory cytokines. Histamine, a main content of granules in mast cells, exerts many effects related to the immediate phase of allergic inflammation including vasodilatation, increased vascular permeability, tissue edema, contraction of bronchial and intestinal smooth muscle, and increased mucus production.7) Mast cell degranulation can also be elicited by the basic secretagogues. The most potent secretagogues include the compound 48/80, which is a mixed polymer of phenethylamine cross-linked by formaldehyde and a high dose of which induces almost a 90% release of histamine from mast cells. 8) Thus, compound 48/80 has been used as a direct and convenient reagent to study the mechanism of allergy and anaphylaxis. 7)In this study, we demonstrate the Agaricus blazei water extract (ABWE) inhibits compound 48/80-induced systemic anaphylaxis-like reaction, ear swelling response, and passive cutaneous anaphylaxis-like reaction in mice. We also show that ABWE reduces anti-dinitrophenyl (DNP) IgE-mediated passive cutaneous anaphylaxis. Afterward, the inhibitory effect of ABWE on compound 48/80-induced mast cell activation is elucidated, as reported previously. 9) MATERIALS AND METHODS MaterialsCompound 48/80, disodium cromoglycate (DSCG) theophylline, anti-DNP IgE, DNP-human serum albumin (HSA), bovine serum albumin (BSA) and HEPES were purchased from Sigma Chemical Co. (St. Louis, MO, U.S.A.). Percoll solution was purchased from Pharmacia (Uppsala, Sweden).Experimental Animals Male ICR mice (6 week old, 25-30 g) and Sprague-Dawley rats (8 week old, 230-280 g) were purchased from Damool Science (Daejeon, Korea). Animals were housed 3-5 per cages in laminar airflow cabinet maintained at 22Ϯ1°C and relative humidity of 55Ϯ10% throughout the study. Animal Research Committee of Chonbuk National University approved the animal study in accordance with the guidelines of the National Institutes of Health (NIH publication #85-23, 1985).Preparation of Agaricus blazei Water Extract (ABWE) Agaricus blazei water extract used in this study was provided from Dr. Yung Hyun Choi (Department of Biochemistry, Dongeui University, Busan, ...

Andosan?-An Anti-Allergic and Anti-Inflammatory Ingredient Prepared from Agaricus blazei Mushroom

“…Our report agrees with AbM-induced anti-inflammatory and antiallergic effects found in mouse bone marrow-derived mast cells [71], the inhibitory effect on mast cell-mediated anaphylaxis-like reactions in mice [72], and the amelioration of skewed Th1/Th2 balance in asthmatic [66] and allergic mice [52]. On the other hand, it disagrees with reports on proatherogenic and proinflammatory effects of AbM in a mouse model for atherosclerosis [73] and a clinical randomized trial that found no immunomodulatory effect in elderly women who received dried AbM extract [74].…”

Pycnogenol® inhibits immunoglobulin E‐mediated allergic response in mast cells

“…45 Ca uptake. The calcium uptake of mast cells was measured according to the method described by Choi et al (2006). Purified mast cells were resuspended in HEPES-Tyrode buffer containing 45 Ca (1.5 mCi/mL; 1 Ci = 3.7 ¥ 10 10 becquerels; Perkin-Elmer Life Sciences, MA, USA) and incubated at 4°C for 10 min.…”

Curcumin Inhibits Compound 48/80 Induced Systemic Anaphylaxis

“…Mice (n=5/group) received an intraperitoneal injection of compound 48/80 (1, 4 and 8 mg/kg, i.p ) as described previously [15][16]. Curcumin (5, 10 and 20 mg/kg, i.p ) was administered an hour prior to administration of compound 48/80.…”

“…In addition, previous studies demonstrated that curcumin had an inhibitory effect on histamine release from mast cells triggered by IgE, calcium ionophore A 23187, or concanavalin A [13][14][15]. Studies have suggested that curcumin has downregulated Th2 response and reduced lung inflammations in latex sensitized mice suggesting possible role of curcumin in controlling allergic disorders [16]. Studies demonstrate that known mast cell stabilizer, DSCG has mast cell stabilizing activity after nonimmunological stimulations [17].…”