Inhibitory effects of curcumin on passive cutaneous anaphylactoid response and compound 48/80-induced mast cell activation

Abstract

Mast cells participate in allergies and inflammation by secreting a variety of pro-inflammatory mediators. Curcumin, the active component of turmeric, is a polyphenolic phytochemical with anti-tumor, anti-inflammatory, anti-oxidative, and anti-allergic properties. The effects of curcumin on compound 48/80-induced mast cell activation and passive cutaneous anaphylactoid reactions are unknown. In this report, we investigated the influences of curcumin on the passive cutaneous anaphylactoid response in vivo and compound 48/80-induced mast cell activation in vitro. The mechanism of action was examined by calcium uptake measurements and cAMP assays in mast cells. Curcumin significantly attenuated the mast cell-mediated passive cutaneous anaphylactoid reaction in an animal model. In agreement with this in vivo activity, curcumin suppressed compound 48/80-induced rat peritoneal mast cell (RPMC) degranulation and histamine release from RPMCs. Moreover, compound 48/80-elicited calcium uptake into RPMCs was reduced in a dose-dependent manner by curcumin. Furthermore, curcumin increased the level of intracellular cAMP and significantly inhibited the compound 48/80-induced reduction of cAMP in RPMCs. These results corroborate the finding that curcumin may have anti-allergic activity.

Antiallergic effect of KOB03, a polyherbal medicine, on mast cell-mediated allergic responses in ovalbumin-induced allergic rhinitis mouse and human mast cells

“…Therefore, mast cell degranulation has been considered a major target for effective anti-allergic drugs. Mast cell degranulation can be elicited by the synthetic compound 48/80, which has therefore been used as a direct reagent to study the mechanism of anaphylactic shock (Sur and Scandale, 2010;Choi et al, 2010). In the present study, KOB03 attenuated compound 48/80-induced release of histamine from mast cells by inhibiting their degranulation.…”

Curcumin Inhibits Compound 48/80 Induced Systemic Anaphylaxis

“…Recently Anaphylactoid response induced by compound48/80 has also been reported [26] with similar mechanism. Earlier studies on Curcumin [12] suggest that most of the compounds develop anti-allergic activities through mechanisms related to both anti-oxidative and non anti-oxidation activities.As reported earlier that curcumin has no toxic effects.…”

Curcumin Inhibits the Activation of Immunoglobulin E-Mediated Mast Cells and Passive Systemic Anaphylaxis in Mice by Reducing Serum Eicosanoid and Histamine Levels

“…Furthermore, it has been reported that curcumin suppresses IgE or compound 48/80 induced passive cutaneous anaphylaxis (Suzuki et al ., 2005; Lee et al ., 2008; Choi et al ., 2010). However, no report has been issued on the effect of curcumin on PSA in mice.…”