Initial biocompatibility and enhanced osteoblast response of Si doping in a porous BCP bone graft substitute

Abstract

Granular shape biphasic calcium phosphate (BCP) bone grafts with and without doping of silicon cations were evaluated in regards to biocompatibility and MG-63 cellular response. To do this we studied Cellular cytotoxicity, cellular adhesion and spreading behavior and cellular differentiation with alizarin red S staining. Gene expression in MG-63 cells on the implanted bone substitutes was also examined at different time points using RT-PCR. In comparison, the Si-doped BCP granule showed more cellular viability than the BCP granule without doping in MTT assay. Moreover, cell proliferation was much higher when Si doping was employed. The cells grown on the silicon-doped BCP substitutes had more active filopodial growth with cytoplasmic webbing that proceeded to the flattening stage, which was indicative of well cellular adhesion. When these cells were exposed to Si-doped BCP granules for 14 days, well differentiated MG-63 cells were observed. Osteonectin and osteopontin genes were highly expressed in the late stage of differentiation (14 days), whereas collagen type I mRNA were found to be highly expressed during the early stage (day 3). These combined results of this study demonstrate that silicon-doped BCP enhanced osteoblast attachment/spreading, proliferation, differentiation and gene expression.

Silicon bioceramic loaded with vancomycin stimulates bone tissue regeneration

“…MG63 cell line has been widely used to validate the biocompatibility in terms of cell proliferation and activity . Cells seeded onto 10%‐Si‐β‐TCP responded better than cells deposited on the β‐TCP pure material as shown in Figure .…”

A Novel Silicon-Based Electrochemical Treatment to Improve Osteointegration of Titanium Implants

“…Moreover, it has been demonstrated that the incorporation of silicon into biocompatible materials can influence the cellular response at the bone-implant interface and thus affects bone formation (14,15).…”

“…The incorporation of specific doping elements such as phosphate and calcium is known to positively influence bone growth and cell differentiation (2,(5)(6)(7)(8). Moreover, silicon is known to play a primary role in the calcification of bone matrix, skeletal tissue development and bone metabolism (12,14,15).…”

Scaffold design and characterisation for osteochondral tissue regeneration

“…Osteoblasts do not divide. They give rise to osteocytes, remain as osteoblasts, or return to the state of osteoprogenitor cells from which they derived (7,8,9). An osteoblast becomes an osteocyte when the cell is encased by osteoid matrix that it synthesizes itself.…”

“…The elastic moduli present a slight decrease after 12 weeks of degradation from 3.4 to 2.9 MPa (Table 3-6). (8) Where k is the intrinsic permeability, t the specimen thickness, A the cross-sectional area, Q is the volumetric flow rate, ∆p is the pressure difference across the sample and µ the dynamic fluid viscosity (deionized water at 10 -3 Pa). All the samples were immersed in PBS solution during 48 hours before testing.…”