Initial Experimental Characterization of a New Ultra-High Resolution FTICR Cell with Dynamic Harmonization

Abstract

A new Fourier transform ion cyclotron resonance (FTICR) cell based on completely new principles of formation of the effective electric potential distribution in Penning type traps, Boldin and Nikolaev (Proceedings of the 58th ASMS Conference, 2010), Boldin and Nikolaev (Rapid Commun Mass Spectrom 25:122-126, 2011) is constructed and tested experimentally. Its operation is based on the concept of electric potential space-averaging via charged particle cyclotron motion. Such an averaging process permits an effective electric force distribution in the entire volume of a cylindrical Penning trap to be equal to its distribution in the field created by hyperbolic electrodes in an ideal Penning trap. The excitation and detection electrodes of this new cell are shaped for generating a quadratic dependence on axial coordinates of an averaged (along cyclotron motion orbit) electric potential at any radius of the cyclotron motion. These electrodes together with the trapping segments form a cylindrical surface like in a conventional cylindrical cell. In excitation mode this cell being elongated behaves almost like an open cylindrical cell of the same length. It is more effective in ion motion harmonization at larger cyclotron radii than a Gabrielse et al.-type (Int J Mass Spectrom Ion Processes 88:319-332, 1989) cylindrical cell with four compensation sections. A mass resolving power of more than twenty millions of reserpine (m/z 609) and more than one million of highly charged BSA molecular ions (m/z 1357) has been obtained in a 7T magnetic field.

Azaspiracid variability of Azadinium poporum (Dinophyceae) from the China Sea

“…High resolution mass spectra were acquired with a solarix XR Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR-MS; Bruker Daltonik GmbH, Bremen, Germany) equipped with a 12 T refrigerated actively shielded superconducting magnet (Bruker Biospin, Wissembourg, France), a dual ion source and Paracell analyzer cell (Nikolaev et al, 2011). The samples were ionized by electrospray ionization in positive ion mode (Bruker Daltonik GmbH, Bremen, Germany).…”

21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: A National Resource for Ultrahigh Resolution Mass Analysis

“…To the best of our knowledge, this result represents the highest resolving power ever reported for BSA, and promises high performance analysis of even larger proteins [30,31]. We processed Figure 3 in magnitude mode and without apodization for direct comparison with prior work [10]. Note that the data shown in Figure 3 is the result of a single acquisition, whereas the prior record was the result of 100 averaged scans.…”

“…The mass spectrometer combines a modified Velos Pro [6][7][8] (ThermoFisher Scientific, San Jose, CA, USA) with an NHMFL-designed external linear quadrupole ion trap, quadrupole ion transfer optics, and novel dynamically harmonized ICR ion trap (DHC) [9,10]. The Velos Pro front end offers high sensitivity, efficient ion isolation, precise control of ion number, and both collisional dissociation and front-end electron transfer dissociation (FETD; we use the simpler term BETD^here).…”

Performance of Orbitrap Mass Analyzer at Various Space Charge and Non-Ideal Field Conditions: Simulation Approach

“…For a long long time, these demands were only met by the Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR) [1]. However, while providing for unsurpassed performance, especially with recently introduced dynamically harmonized cells [2,3], this method is not free of some drawbacks like the need for high magnetic field cryomagnets incurring significant usage expenses. For this reason, there is growing interest in another design of Fourier transformbased spectrometers-the recently invented orbital ion trap having performance comparable to FT-ICR instruments, which have ion traps without dynamic harmonization.…”