Initial general management and surgery of six extensively burned children treated with cultured epidermal autografts

Saulnier, J.-P.; Ainaud, P.; H, Lebever; Stephanazzi, J.; Lecoadou, Anne; Carsin, H.

doi:10.1016/s0022-3468(99)90083-0

Cited by 14 publications

(11 citation statements)

References 21 publications

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“…Thirdly, the production of CEA is labor intensive and the process of growing the sheets of cells is expensive (Fraulin et al, 1998). Chalumeau et al (1999) estimated that the cost of this treatment was approximately US$80 000 for children and US$110 000 for adults.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

2006

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A previous model developed by the authors investigates the growth patterns of keratinocyte cell colonies after they have been applied to a burn site using a spray technique. In this paper we investigate a simplified one-dimensional version of the model. This model yields travelling wave solutions and we analyse the behaviour of the travelling waves. Approximations for the rate of healing and maximum values for both the active healing and the healed cell densities are obtained.

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Section: Introductionmentioning

confidence: 99%

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

2006

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“…25,26,61 In patients with massive burns, CEA was indeed life saving especially if there were no other treatment options. 21,30 However, after more than four decades of research, results from CEA application remained unpredictable. 2,79 Good CEA take was sometimes the exception rather than the norm.…”

Section: Discussionmentioning

confidence: 99%

“…[10][11][12][13][14][15][16][17][18][19][20] In 18 publications, CEA was used to treat pediatric burn injuries only. 11,15,16,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34] Notably, the largest clinical series published by Oshima et al included 430 patients with acute burn injuries, revision of burn scars, giant naevi, and tattoos. 17 In Matsumura et al's 6 year multi-center surveillance on the application of JACE ® (Japan Tissue Engineering Co, Japan), 515 patients were enrolled to undergo CEA treatment with 216 patients included in the week 4 efficacy analysis.…”

Section: Patient Demographicsmentioning

confidence: 99%

“…At least 18 centers preferred only full thickness hair bearing skin from donor sites such as axilla, groin, and scalp. 13,15,20,21,23,25,30,[37][38][39][40][41][42][43][44][45][46][47] In general, culturing techniques were based on those described by Rheinwald and Green in 1975, and keratinocytes cultured on a feeder layer of lethally irradiated 3T3 murine fibroblasts. 2 Braye et al used irradiated human foreskin fibroblasts instead of murine fibroblasts however, the most significant departure from Green's method was perhaps Pittelkow's two-phase technique.…”

Section: Cea Manufacturing and Deliverymentioning

confidence: 99%

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A systematic review: Current trends and take rates of cultured epithelial autografts in the treatment of patients with burn injuries

Chong

Akbarzadeh³

et al. 2019

Wound Repair Regeneration

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Several issues persist in clinical translation and application of cultured epithelial autografts during treatment of patients with massive burn injuries. The aim of this systematic review is to determine (1) current practice and trends in clinical application and (2) clinical efficacy of cultured epithelial autografts. A structured literature search was performed in Ovid MEDLINE from 1946 and Ovid EMBASE from 1974 until present. All published peer‐reviewed randomized or non‐randomized clinical studies, cohort studies, prospective, or retrospective series involving human application of cultured epithelial autografts in the setting of burn injury were included. From 7,267 studies initially identified, 77 studies were included in the analysis. In 96% (74/77) of these series, the sample size was less than 100 patients. In 76.6% (59/77) publications, average burn treated exceeded 40% total body surface area. Overall, cultured epithelial autograft take rates reported in the literature were inconsistent and varied significantly from 0 to 100%. There was a recent trend for co‐application of cultured grafts with autologous skin grafts, achieving relatively high and consistent take rates of 73–96%. Results from cultured epithelial autograft application remained unpredictable. This technology remains an adjunct or biological dressing, and not an alternative to conventional split skin graft. However, it has contributed to wound closure and it has been life saving in selected circumstances. Skin tissue engineering should continue as the clinical need for skin replacement is foreseeable into the future.

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“…Also, the production of CEA is labor intensive and the process of growing the sheets of cells is expensive (Fraulin et al, 1998). Chalumeau et al (1999) estimated that the cost of this treatment was approximately US $80,000 per child and US $110,000 per adult during the period from 1990 to 1995.…”

Section: Wound Repairmentioning

confidence: 99%

Mathematical Modelling of Aerosolised Skin Grafts Incorporating Keratinocyte Clonal Subtypes

et al. 2006

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Severe burns can be very traumatic for the patient, and while burns caused by industrial or domestic accidents are common, there are also increasing numbers of burns associated with terrorism. A novel technique to assist in the healing process is to spray skin cells, keratinocytes, that are cultured from the patient's own tissue, directly onto the burn site. This process involves taking some undamaged skin from the patient, allowing the skin cells to proliferate rapidly in the laboratory over a period of 5-10 days, harvesting and separating the cells and then spraying them onto the burn. This paper deals with keratinocytes that have been cultured in vitro for a short period of time (early passage cultured cells). The spraying process has yet to be optimised with respect to the seeding density required for fastest re-epithelisation and thus there is a need for this process to be modelled. In this paper, we review some of the skin biology and develop a mathematical model of the growth patterns of cell colonies after they have been applied using a aerosolised technique. The model allows us to predict coverage over time and can be used as a decision support tool for clinicians.

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Initial general management and surgery of six extensively burned children treated with cultured epidermal autografts

Cited by 14 publications

References 21 publications

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

Analysis of Travelling Waves Associated with the Modelling of Aerosolised Skin Grafts

A systematic review: Current trends and take rates of cultured epithelial autografts in the treatment of patients with burn injuries

Mathematical Modelling of Aerosolised Skin Grafts Incorporating Keratinocyte Clonal Subtypes

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