Initial In Vitro Biological Response to Contemporary Endodontic Sealers

Bouillaguet, Serge; Wataha, John C.; Tay, Franklin R.; Brackett, Martha Goël; Lockwood, Petra E.

doi:10.1016/j.joen.2006.05.006

Cited by 111 publications

(139 citation statements)

References 32 publications

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“…Other studies have corroborated these findings, showing that Epiphany or Real Seal was cytotoxic at the 24-hour time interval. 8,11,21,22 However, in contrast to our results, other authors 23 found that the cytotoxicity of this material increases with time. It is important to note, however, that the longest time used in other works was considerably lower than those used in the present methodology.…”

Section: Discussioncontrasting

confidence: 99%

“…20 Even though the literature has described an increase on this material's cytotoxicity with time after mixing, which could be attributed to the release of silver particles, 8 in the present work GuttaFlow was less cytotoxic than all other sealers tested, at most exposure times.…”

Section: Discussioncontrasting

confidence: 55%

“…7 The slight expansion of these materials during setting is conjectured to result in an improved apical seal of the root canal system. 8 Regardless of their composition, root canal sealers should be biocompatible and present satisfactory physico-chemical properties. International standards 9 advise that the assessment of biocompatibility is critical prior to the clinical use of rootfilling materials.…”

Section: Introductionmentioning

confidence: 99%

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Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts

2012

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The aim of this work was to evaluate the effects of different times of extraction on the cytotoxicity of six representatives of different root canal sealer groups-Real Seal SE, AH Plus, GuttaFlow, Sealapex, Roth 801, and ThermaSeal Plus-with human gingival fibroblasts. The materials were prepared according to manufacturers' specifications, and were incubated in culture medium (DMEM) at 37°C for 1, 7, 14, 21, and 28 days, with daily washing, to simulate periodontal ligament clearance. Human fibroblasts were exposed to the final extracts at 24 hours, and cell viability was determined by MTT assay, with exposure to unconditioned DMEM as a negative control. Statistical analysis comparing cytotoxicities at each exposure time was performed by ANOVA with Scheffé adjustment for multiple comparisons at a 95% confidence level. Results indicated that GuttaFlow was significantly less cytotoxic than all other sealers (p < 0.05) at 1 day of extraction. After 7 days of extraction, cell viability for GuttaFlow was significantly increased as compared with that of all groups except sealer AH Plus. At day 14, cytotoxicity of Sealapex was significantly higher than that of all other sealers (p < 0.05). At days 21 and 28, there were no significant differences in cytotoxicity among sealer groups. All materials presented some level of cytotoxicity to fibroblasts, while GuttaFlow was the least cytotoxic sealer tested. However, the cytotoxicity of all materials seemed to decrease similarly in a time-dependent manner.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts

2012

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“…2,130 However, some studies have shown that Resilon is more cytotoxic than gutta-percha and its cytotoxicity increases after 48 hours. 131,132 It has been suggested that cytotoxicity of Resilon + Epiphany, mainly due to Epiphany, decreases after 2 days to reach a level comparable to commonly used root canal sealers. 133 In one investigation Epiphany was more cytotoxic than conventional materials 130 but in another one they exhibited the same results.…”

Section: Introductionmentioning

confidence: 99%

Resilon: A Comprehensive Literature Review

Lotfi,

Ghasemi,

Rahimi

et al. 2013

Journal of Dental Research

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“…Dental monomers can affect intracellular pathways leading to cell survival or cell death 8,9) . In this context, TEGDMA, UDMA, HEMA and BisGMA, interfere with cell metabolism and redox imbalance and seem to play an important role to reactive oxygen species formation and induction of cell apoptosis 6,10,11) .…”

Section: Introductionmentioning

confidence: 99%

Effects of a dental adhesive on cell cycle regulatory proteins

et al. 2013

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Dental bonding agents may affect the cell cycle patterns and induce cell cycle arrest by blocking its progression. This study tested the cell cycle effects through cyclin-dependent kinase (cdc2) and Rb phosphorylation. Human lung fibroblasts (MRC5) were used for the experiments. The bonding agent tested was the total-etch XP bond. Extracts of the bonding agent were prepared and serial dilutions were tested. The effects of the bonding agent on cell survival, proliferation and DNA synthesis were tested by the SRB and BrdU assays. Analysis of cell cycle distribution was performed by flow cytometry. XP bond exhibited strong inhibition of DNA synthesis and after 48 h of exposure cells were accumulated in the G2/M phase. Cells exposed to the half maximal cell growth inhibitory concentration (IC 50 ) showed an increase in cdc2 kinase and Rb phosphorylation. The results most likely indicate mutagenic effect of the tested agent.

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Initial In Vitro Biological Response to Contemporary Endodontic Sealers

Cited by 111 publications

References 32 publications

Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts

Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts

Resilon: A Comprehensive Literature Review

Effects of a dental adhesive on cell cycle regulatory proteins

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