Initial purification of recombinant botulinum neurotoxin fragments for pharmaceutical production using hydrophobic charge induction chromatography

Modification of Martini force field for molecular dynamics simulation of hydrophobic charge induction chromatography of lysozyme

“…Therefore, HCIC has many advantages over traditional HIC, such as moderate operation condition, high adsorption capacity, and easy elution of bound proteins. Till now, HCIC has been successfully used in the separation and purification of antibodies [5][6][7][8][9][10][11][12] and various other proteins [7,[13][14][15]. In order for better understanding of HCIC processes for protein separations, fundamental studies have been extensively carried out [10,[16][17][18][19][20][21].…”

Molecular dynamics simulation of the effect of ligand homogeneity on protein behavior in hydrophobic charge induction chromatography

“…Consequently, HCIC adsorbents can be made at very high ligand densities to achieve high adsorption capacity with little difficulty in the elution of bound proteins. To date, it has been applied in the separation and purification of antibodies [3,[5][6][7][8][9][10] and various other proteins [7,[11][12][13][14].…”

Ligands for mixed-mode protein chromatography: Principles, characteristics and design

“…Mixed-mode chromatography has found utilities in a number of protein purification processes (Weatherly et al, 2002;Coulon et al, 2004;Mowry et al, 2004;Brenac et al, 2005;Charoenrat et al, 2006;Arakawa et al, 2009). It is advantageous for its salt-independent adsorption, facile elution and unique selectivity.…”