Initial stage of development and migratory behavior of Toxocara canis larvae in BALB/c mouse experimental model

Camparoto, Marjori Leiva; Fulan, B; Cm, Colli; Ml, Paludo; Falavigna-Guilherme, Ana Lúcia; Ma, Fernández

doi:10.4238/vol7-2gmr443

Cited by 18 publications

(13 citation statements)

References 19 publications

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“…It has been observed that larvae can invade the mouse CNS during the initial phase of infection; with cumulative infection doses and progress of infection, the probability of additional organ involvement increases (Sprent 1955;Dunsmore et al 1983;Good et al 2001;Hamilton et al 2006;Camparoto et al 2008). Neurotoxocarosis can be manifested by behavioral changes: the experimentally infected mice were less aggressive, less explorative, and showed some impairment in learning and memory (Cox and Holland 1998;Cox and Holland 2001a, b;Hamilton et al 2006).…”

Section: Introductionmentioning

confidence: 94%

Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS

et al. 2011

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Using a small animal imaging system, migratory activity of Toxocara canis larvae stained by carboxyfluorescein succinimidyl ester (CFSE) was observed post primary infection (PPI) and post reinfection (PR) of BALB/c mice. Each infection was performed with 1,000 larvae per mouse. Primary infections were performed with labeled larvae, while for challenge infections the reinfecting larvae were stained by CFSE. The worm burden in mouse organs was determined during a period from 6 h to 21 days and 4 months PPI and PR. In comparison with primary infections that led to the first larvae appearance in the brain after 60 h, greatly accelerated migration of the parasites administered 3 weeks PPI to the CNS and eyes of challenged mice was noted-in both organs the larvae appeared 6 h PR. In all challenged mice, reinfecting larvae prevailed in the resident parasite population. Preliminary experiments with Toxocara cati larvae also revealed early brain involvement in primarily infected mice. Staining of T. canis larvae by CFSE had no effect on the development of a humoral antibody response against T. canis excretory-secretory antigens. In ELISA, elevated levels of specific IgG and IgG1 were noted on day 14 PPI and the levels of antibodies increased till the end of experiment. Reinfection induced an increase in the levels of both antibodies. In terms of optical density, IgG1 antibodies gave higher values in all sera examined. In ELISA for IgG antibodies, an increase in the avidity index of around 50% was detected 1 month PPI; higher-avidity antibodies were also detected in sera of reinfected animals.

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Section: Introductionmentioning

confidence: 94%

Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS

et al. 2011

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“…This rodent may also be infected with other helminthic and protozoan parasites facilitating the study of concomitant infections 6,20 .…”

Section: Introductionmentioning

confidence: 99%

IgG Antibody responses in mice coinfected with Toxocara canis and other helminths or protozoan parasites

Lescano

Nakhle

Ribeiro³

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe immune response expressed by IgG antibodies in BALB/c mice experimentally infected with Toxocara canis, was studied with the aim of verifying the possible in vivo cross-reactivity between antigens of T. canis and other parasites (Ascaris suum, Taenia crassiceps, Schistosoma mansoni, Strongyloides venezuelensis and Toxoplasma gondii). Experiments included three groups of mice: one infected only by T. canis, another with one of the other species of parasites and a third concomitantly infected with T. canis and the other species in question. Animals were bled by orbital plexus at 23, 38 and 70 days post infection (p.i.). Sera were analyzed for anti-Toxocara antibodies by ELISA and Immunoblotting, using excretion-secretion antigens (ES), obtained from culture of thirdstage larvae of T. canis. For all experiments a control group comprised by ten non-infected mice was used. Only in the case of A. suum infection, in these experimental conditions, the occurrence of cross-reactivity with T. canis was observed. However, in the case of co-infection of T. canis -S. mansoni, T. canis -S. venezuelensis and T. canis -T. crassiceps the production of anti-Toxocara antibodies was found at levels significantly lower than those found in mice infected with T. canis only. Co-infection with S. mansoni or S. venezuelensis showed lower mortality rates compared to what occurred in the animals with single infections. Results obtained in mice infected with T. canis and T. gondii showed significant differences between the mean levels of the optical densities of animals infected with T. canis and concomitantly infected with the protozoan only in the 23 rd day p.i.

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“…La afectación del sistema nervioso central (SNC) es inusual 2,7,8 y comprende la meningitis 2,8,9 , encefalitis 8,10 , mielitis 2,7,9,10-13 , o radiculitis 8 . Sin embargo, en modelos animales, larva migrans migra con frecuencia al SNC 14 .…”

Section: Discussionunclassified

Parálisis de Bell y dolor facial asociados con infección por Toxocara sp

Bachtiar

Auer

Finsterer

2012

Rev. chil. infectol.

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Initial stage of development and migratory behavior of Toxocara canis larvae in BALB/c mouse experimental model

Cited by 18 publications

References 19 publications

Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS

Toxocara canis larvae reinfecting BALB/c mice exhibit accelerated speed of migration to the host CNS

IgG Antibody responses in mice coinfected with Toxocara canis and other helminths or protozoan parasites

Parálisis de Bell y dolor facial asociados con infección por Toxocara sp

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