1997
DOI: 10.1146/annurev.immunol.15.1.453
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Initiation and Processing of Signals From the B Cell Antigen Receptor

Abstract: Current models of signal transduction from the antigen receptors on B and T cells still resemble equations with several unknown elements. Data from recent knockout experiments in cell lines and mice contradict the assumption that Src-family kinase and tyrosine kinases of the Syk/Zap-70 family are the transducer elements that set signaling from these receptors in motion. Using a functional definition of signaling elements, we discuss the current knowledge of signaling events from the BCR and suggest the existen… Show more

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Cited by 417 publications
(320 citation statements)
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“…In each case, the lipid rafts concentrate the Src-family kinase (Lyn, Fyn and Lck among others) that initiates signaling and the immune receptors are excluded from the lipid rafts in resting or inactivated cells [32,33]. The binding of multivalent ligands induces the oligomerization of these receptors, resulting in the translocation of the receptors into lipid rafts where the tyrosine residues within the immunoregulatory tyrosine activation motifs (ITAMs) are phosphorylated [27,34]. The CD40 receptor could be constitutively associated with lipid rafts at a level that is cell type dependent [20].…”
Section: Discussionmentioning
confidence: 99%
“…In each case, the lipid rafts concentrate the Src-family kinase (Lyn, Fyn and Lck among others) that initiates signaling and the immune receptors are excluded from the lipid rafts in resting or inactivated cells [32,33]. The binding of multivalent ligands induces the oligomerization of these receptors, resulting in the translocation of the receptors into lipid rafts where the tyrosine residues within the immunoregulatory tyrosine activation motifs (ITAMs) are phosphorylated [27,34]. The CD40 receptor could be constitutively associated with lipid rafts at a level that is cell type dependent [20].…”
Section: Discussionmentioning
confidence: 99%
“…Guinea pig complement and DMEM were purchased from Life Technologies (Grand Island, NY). Fab of goat anti-mouse IgM, Cy3-conjugated Fab of goat anti-mouse IgM, biotin-conjugated F(abЈ) 2 of goat anti-mouse IgM ϩ IgG, and avidin and tetramethylrhodamine isothiocyanate-conjugated goat anti-rat IgG were from Jackson ImmunoResearch Laboratories (West Grove, PA). The 1D4B, a mAb specific for lysosomal-associated membrane glycoprotein-1 (LAMP-1), and TIB219, a mAb specific for mouse transferrin receptor (TfR), were obtained from hybridomas purchased from American Type Culture Collection (Manassas, VA).…”
Section: Abs and Reagentsmentioning
confidence: 99%
“…Cells were incubated with 20 g/ml of biotin-conjugated F(abЈ) 2 of goat anti-mouse IgM ϩ IgG (B-anti-Ig), as a paucivalent Ag, for 30 min at 4°C before warming. For polyvalent Ag cross-linking, the BCR was crosslinked with 20 g/ml of B-anti-Ig for 10 min, followed by 20 g/ml of avidin for an additional 20 min at 4°C before warming.…”
Section: Ab Engagement Of the Bcrmentioning
confidence: 99%
“…During affinity maturation, the interaction of Ags with the BCR complex is presumably necessary for the selection of highaffinity Abs, which may elicit a critical BCR-signaling cascade. BCR stimulation triggers tyrosine phosphorylation of the ITAM of the B cell-specific molecules as CD79a (12) and CD79b (13), where src-type tyrosine kinases and adaptor molecules accumulate through tyrosine phosphorylation of their SH2 domains (14). The nonreceptor-type tyrosine kinase Lyn is expressed in lymphocytes and plays a role as a proximal tyrosine kinase in BCR-mediated signal transduction, including Syk tyrosine kinase, leading to the activation of Btk, PI3K, and phospholipase Cg (15).…”
mentioning
confidence: 99%