Initiation and propagation of Glycine max L. Merr.: Plants from tissue-cultured epicotyls

Abstract

A successful technique for the initiation and proliferation of shoots from epicotyl tissue of soybean, Glycine max, has been developed and is described. Fertile plants were recovered. Seeds were germinated on Murashige and Skoog medium containing 5#M benzyladenine. Explanted epicotyl sections were induced to form callus and shoots on Schenk and Hildebrandt medium containing 5.2mM monobasic ammonium phosphate, 74#M 3-aminopyridine, and 20/~M kinetin for five weeks. Shoot proliferation was maintained on N6 medium containing 1.75 mM ammonium sulfate, 2.1 nM picloram, and 0.1 #M benzyladenine. Shoots rooted on Gamborg's B5 medium without growth regulators. Shootforming cultures were maintained for 60 months. Although all varieties tested produced shoots, some variation in numbers of shoots obtained was observed.

Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr).

“…Soybean repetitive organogenesis has been reported before either by continuous culture on BA supplemented medium (Wright et al 1987a(Wright et al , 1987bShetty et al 1992) or by using alternate cycles of culture on TDZ and BA supplemented media (Barwale and Widholm 1990). However, in these repetitive organogenic systems the tissue was maintained in the multiple shoot stage.…”

“…However, in these repetitive organogenic systems the tissue was maintained in the multiple shoot stage. Seven shoots/explant were obtained when continuously cultured on BA supplemented medium every month and only 2% of the shoots developed into normal plants (Wright et al 1987a(Wright et al , 1987b. No exact details were given when shoots were cultured on alternate cycles of BA and TDZ (Barwale and Widholm 1990).…”

“…Organogenesis is less genotype dependent and has become routine in several laboratories (Wright et al 1986(Wright et al , 1987a(Wright et al , 1987bBarwale et al 1986;Dan and Reighceri 1998). The regeneration is based on proliferation of meristems in the cotyledonary node.…”

Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)

“…, BA (Zia et al, 2010;Ma and Wu, 2008;Dan and Reichert, 1998;Kaneda et al, 1997;Malik and Saxena, 1992), zeatin (Zia et al, 2010;Kim et al, 2001), 그리고 kinetin (Marele, 2013;Joyner et al, 2010;Wright et al, 1987) (Klink et al, 2008;Hiraga et al, 2007;Lim et al, 2005;Ko and Korban, 2004;Hofmann et al, 2004;Santarém et al, 1997;Finer and Nagasawa, 1988)이 주로 이용되며, 자엽 의 성숙 정도에 따라 다른 결과를 보인다. 그 밖에 품종 간 유전 자형 (Yang et al, 2009;Hiraga et al, 2007;Kita et al, 2007;Hofmann et al, 2004;Baily et al, 1993;Finer and Nagasawa, 1988;Komatsuda and Oyama, 1988), pH (Hofmann et al, 2004;Lazzeri et al, 1987), 배지 및 고형물질의 종류 (Rajasekaran and Pellow, 1997;Santarém et al, 1997 The explants were cultured on MS medium containing 0, 1, 2 and 4 ㎎/L BA for 4 weeks.…”

Adventitious Shoot Formation from Hypocotyl Sections of Mature Soybean Seeds.

“…There have been several reports of shoot formation from cotyledonary nodes and plumules , Yang et al 1990), epicotyl-hypocotyl areas (Shetty et al 1992), epicotyls (Wright et al 1987a), primary leaf nodes (Kim et al 1990), and stem nodes ) of 3 to 28-day-old soybean seedlings.…”