Injectable bone using chitosan-alginate gel/mesenchymal stem cells/BMP-2 composites

Park, Dong Joon; Choi, Byung Ho; Zhu, Shi Jiang; Huh, Jin Young; Kim, Byung Young; Lee, Seoung Ho

doi:10.1016/j.jcms.2004.05.011

Cited by 136 publications

(58 citation statements)

References 26 publications

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“…Alginate-Chitosan Bead Culture. Chitosan precipitation was done by slightly modifying the procedure of Park et al 24 Briefly, a 3.3% chitosan solution was produced by dissolving 0.25 g chitosan (Sigma Aldrich, Germany) in 7.5 mL of 0.2 M acetic acid and autoclaved at 121 C for 10 min. 720 lL of 0.1 M NaOH was mixed with 500 lL of 3.3% chitosan solution in a rotator for 5 min.…”

Section: Cell and Tissue Culturementioning

confidence: 99%

Pellet culture elicits superior chondrogenic redifferentiation than alginate‐based systems

Bernstein

Dong

Corbeil³

et al. 2009

Biotechnology Progress

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Although pellet culture and encapsulation of chondrocytes into gel-like biomaterials have lead to major advances in cartilage tissue engineering, a quantitative comparative characterization of cellular differentiation behavior during those cultivation procedures has not yet been performed. Our study therefore aimed at answering the following question: is the redifferentiation pathway of chondrocytes altered by slight changes in the type of alginate biomaterial (pure alginate, alginate-fibrin, alginate-chitosan) and how do the cells behave in comparison to biomaterial-free (pellet) three-dimensional culturing? Monolayer-expanded chondrocytes from healthy adult porcine knee joints were cultivated in alginate, alginate-chitosan, alginate-fibrin beads and as pellets up to 4 weeks. Quantitative PCR and Immunohistology were used to assess chondrogenic markers. Alginate-fibrin-encapsulated chondrocytes behaved almost like monolayer chondrocytes. Alginate- and alginate-chitosan encapsulation lead to a low chondrogenic marker gene expression. Although all 3D-cultured chondrocytes showed a considerable amount of Sox9 expression, only pellet cultivation lead to a sufficient Collagen II expression. This puts the usage of alginate-cultivated cartilage tissue engineering constructs under question. Fibrin addition is not beneficial for chondrogenic differentiation. Sox9 and Collagen II behave differently, depending upon the surrounding 3D-environment.

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Section: Cell and Tissue Culturementioning

confidence: 99%

Pellet culture elicits superior chondrogenic redifferentiation than alginate‐based systems

Bernstein

Dong

Corbeil³

et al. 2009

Biotechnology Progress

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“…First, alginate is a biocompatible polysaccharide that is structurally similar to the glycosaminoglycans present in cartilage. [42][43][44] Also, alginate gelation properties allow gel formation directly on bone defects by the simple addition of a calcium chloride solution. Thus, it is a fluid material that can fill the shape of any defect and incorporate various bioactive agents (e.g., proteins and growth factors).…”

Section: Discussionmentioning

confidence: 99%

Ethyl-3,4-Dihydroxybenzoate with a Dual Function of Induction of Osteogenic Differentiation and Inhibition of Osteoclast Differentiation for Bone Tissue Engineering

Kwon

Lee

Koo

et al. 2014

Tissue Engineering Part A

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The current approach in biomaterial design of bone implants is to induce in situ regeneration of bone tissue, thus improving integration of the implants and reducing their failure. Therefore, ethyl-3,4-dihydroxybenzoate (EDHB), which stimulates differentiation of osteoblasts and the resultant bone formation, should be studied. In this study, the osteoinductive ability of EDHB in preosteoblasts and human mesenchymal stem cells was examined. EDHB for future use in bone tissue engineering was evaluated by examination of early markers of differentiation (such as alkaline phosphatase [ALP] activity and collagen type I expression) and late markers of osteoblast differentiation (bone nodule formation). As bone remodeling and implant osteointegration depend not only on osteoblast response but also on interaction of the biomaterial with bone-resorbing osteoclasts, differentiation of osteoclasts in response to the compounds was also observed. For in vivo study, alginate gel comprised of EDHB and cells was transplanted into the back subcutis of mice. Our results show that EDHB might have beneficial effects through regulation of both osteoblast and osteoclast differentiation. Therefore, we suggest that EDHB could be a strong candidate for dual regulation to increase osteoblast differentiation and decrease osteoclast differentiation.

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“…Several controlled release systems promoting the delivery of GFs are combined in strategies with cells, with the goal of acting in a synergistic manner and promoting the enhancement of new tissue formation. Co-encapsulation of GFs and cells in hydrogels [2][3][4][5][6][7][8][9][10] and seeding of stem cells in microparticles or scaffolds loaded with bioactive agents [11][12][13][14][15][16][17][18] are among the most common TE strategies described in literature. Typically, GFs have been included in TE strategies through three main approaches: (1) incorporation within micro-and nano-particles, which can act as supplements for in vitro cell cultures or can be injected into the defect sites, stimulating in situ tissue healing.…”

Section: Single Gf Deliverymentioning

confidence: 99%

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

Santo

Gomes²,

Mano³

et al. 2013

Tissue Engineering Part B: Reviews

105

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The development of controlled release systems for the regeneration of bone, cartilage, and osteochondral interface is one of the hot topics in the field of tissue engineering and regenerative medicine. However, the majority of the developed systems consider only the release of a single growth factor, which is a limiting step for the success of the therapy. More recent studies have been focused on the design and tailoring of appropriate combinations of bioactive factors to match the desired goals regarding tissue regeneration. In fact, considering the complexity of extracellular matrix and the diversity of growth factors and cytokines involved in each biological response, it is expected that an appropriate combination of bioactive factors could lead to more successful outcomes in tissue regeneration. In this review, the evolution on the development of dual and multiple bioactive factor release systems for bone, cartilage, and osteochondral interface is overviewed, specifically the relevance of parameters such as dosage and spatiotemporal distribution of bioactive factors. A comprehensive collection of studies focused on the delivery of bioactive factors is also presented while highlighting the increasing impact of platelet-rich plasma as an autologous source of multiple growth factors.

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Injectable bone using chitosan-alginate gel/mesenchymal stem cells/BMP-2 composites

Cited by 136 publications

References 26 publications

Pellet culture elicits superior chondrogenic redifferentiation than alginate‐based systems

Pellet culture elicits superior chondrogenic redifferentiation than alginate‐based systems

Ethyl-3,4-Dihydroxybenzoate with a Dual Function of Induction of Osteogenic Differentiation and Inhibition of Osteoclast Differentiation for Bone Tissue Engineering

Controlled Release Strategies for Bone, Cartilage, and Osteochondral Engineering—Part II: Challenges on the Evolution from Single to Multiple Bioactive Factor Delivery

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