Injectable extracellular vesicle-released self-assembling peptide nanofiber hydrogel as an enhanced cell-free therapy for tissue regeneration

Zhou, Yijie; Liu, Shuyun; Zhao, Meng; Wang, Chengshi; Li, Ling; Yuan, Yujia; Li, Lan; Liao, Guangneng; Bresette, William; Zhang, Jie; Chen, Younan; Cheng, Jun; Lu, Yanrong; Liu, Jingping

doi:10.1016/j.jconrel.2019.11.003

Cited by 113 publications

(76 citation statements)

References 43 publications

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“…Moreover, proliferation of endothelial cells and angiogenesis were both improved, resulting in reduced chronic renal fibrosis. [135] The angiogenesis-enhancing properties of MSC-EVs have already been demonstrated in vivo, and the administration of MSC-EVs incorporated in matrigel enhanced the angiogenesis even more, due to the sustained release and prolonged residence of the EVs. [200] Age-induced vascular dysfunction was treated with umbilical cord mesenchymal stem cell-derived exosomes (UMSCs-exos) containing miR-675 and encapsulated in silk fibroin hydrogels, in order to be delivered into the ischemic hind-limb of mice.…”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Potential Applications of Advanced Nano/Hydrogels in Biomedicine: Static, Dynamic, Multi‐Stage, and Bioinspired

Ayoubi‐Joshaghani

Seidi

Azizi

et al. 2020

Adv Funct Materials

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Novel advanced hydrogels can provide a versatile platform for controlled delivery and release of various cargos, with a myriad of biomedical applications. These gel-based nanostructures possess good biocompatibility, biodegradability, flexibility, multifunctionality, can respond to internal or external stimuli, and can adapt to their surrounding environment. This new generation of hydrogels is not only capable of serving as targeted drug delivery vehicles, but they can also perform a variety of tasks within living cells and organisms. In this review, advanced hydrogels are classified as static, dynamic, multistage, or bioinspired. They can be used as cell-free gene expression platforms for gene therapy. Administration of nanogel-based sprays can act as an immunovaccine priming macrophages toward the M1 phenotype to avoid cancer recurrence following surgery. Nanogels can also serve as a dual biosensing and capture platform for liquid biopsies, and can recognize and remove circulating cancer cells from the blood of cancer patients.

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Section: Extracellular Vesiclesmentioning

confidence: 99%

Potential Applications of Advanced Nano/Hydrogels in Biomedicine: Static, Dynamic, Multi‐Stage, and Bioinspired

Ayoubi‐Joshaghani

Seidi

Azizi

et al. 2020

Adv Funct Materials

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“…Considering the mechanism responsible for this in vitro in cisplatin-treated human tubular epithelial cells, it was shown that the EVs up-regulated anti-apoptotic genes (B-cell lymphoma extra-large B-cell lymphoma 2 and baculoviral IAP repeat containing 8) and down-regulated genes that contribute in the execution-phase of cell apoptosis (caspase-1, caspase-8 and lymphotoxin alpha) [116]. A significant better EV efficacy followed by improved renal function was noticed when mice BMMSC-EVs were loaded to self-assembling peptide nanofiber hydrogels, for control and targeted release of EVs on the site of mice AKI models after ischaemia-reperfusion [117]. Aside from bone marrow, MSC-EVs from other tissues have been isolated and evaluated for renal regeneration.…”

Section: Kidney Regenerationmentioning

confidence: 99%

Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications

Tsiapalis

O’Driscoll

2020

Cells

227

164

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Mesenchymal stem cells (MSCs) are being extensively investigated for their potential in tissue engineering and regenerative medicine. However, recent evidence suggests that the beneficial effects of MSCs may be manifest by their released extracellular vesicles (EVs); typically not requiring the administration of MSCs. This evidence, predominantly from pre-clinical in vitro and in vivo studies, suggests that MSC-EVs may exhibit substantial therapeutic properties in many pathophysiological conditions, potentially restoring an extensive range of damaged or diseased tissues and organs. These benefits of MSC EVs are apparently found, regardless of the anatomical or body fluid origin of the MSCs (and include e.g., bone marrow, adipose tissue, umbilical cord, urine, etc). Furthermore, early indications suggest that the favourable effects of MSC-EVs could be further enhanced by modifying the way in which the donor MSCs are cultured (for example, in hypoxic compared to normoxic conditions, in 3D compared to 2D culture formats) and/or if the EVs are subsequently bio-engineered (for example, loaded with specific cargo). So far, few human clinical trials of MSC-EVs have been conducted and questions remain unanswered on whether the heterogeneous population of EVs is beneficial or some specific sub-populations, how best we can culture and scale-up MSC-EV production and isolation for clinical utility, and in what format they should be administered. However, as reviewed here, there is now substantial evidence supporting the use of MSC-EVs in tissue engineering and regenerative medicine and further research to establish how best to exploit this approach for societal and economic benefit is warranted.

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“…EVs from various sources have therapeutic potency, among which MSC-derived EVs appear particularly useful in the treatment of diverse conditions, including the treatment of inflammatory disorders of the respiratory system 95 - 98 , heart 99 - 101 , liver 102 - 104 , kidney 105 - 108 , nervous system 109 - 113 , arthrosis 114 - 116 , muscle 117 - 119 and others 120 - 124 ( Table 1 ). The therapeutic action of MSC-EVs is reliant on their transfer of genetic materials and proteins.…”

Section: Applying Ev-based Nanotherapeutics In the Fight Against Inflmentioning

confidence: 99%

Extracellular vesicle-based Nanotherapeutics: Emerging frontiers in anti-inflammatory therapy

Tang

Wang

et al. 2020

Theranostics

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Dysregulated inflammation is a complicated pathological process involved in various diseases, and the treatment of inflammation-linked disorders currently represents an enormous global burden. Extracellular vesicles (EVs) are nanosized, lipid membrane-enclosed vesicles secreted by virtually all types of cells, which act as an important intercellular communicative medium. Considering their capacity to transfer bioactive substances, both unmodified and engineered EVs are increasingly being explored as potential therapeutic agents or therapeutic vehicles. Moreover, as the nature's own delivery tool, EVs possess many desirable advantages, such as stability, biocompatibility, low immunogenicity, low toxicity, and biological barrier permeability. The application of EV-based therapy to combat inflammation, though still in an early stage of development, has profound transformative potential. In this review, we highlight the recent progress in EV engineering for inflammation targeting and modulation, summarize their preclinical applications in the treatment of inflammatory disorders, and present our views on the anti-inflammatory applications of EV-based nanotherapeutics.

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Injectable extracellular vesicle-released self-assembling peptide nanofiber hydrogel as an enhanced cell-free therapy for tissue regeneration

Cited by 113 publications

References 43 publications

Potential Applications of Advanced Nano/Hydrogels in Biomedicine: Static, Dynamic, Multi‐Stage, and Bioinspired

Potential Applications of Advanced Nano/Hydrogels in Biomedicine: Static, Dynamic, Multi‐Stage, and Bioinspired

Mesenchymal Stem Cell Derived Extracellular Vesicles for Tissue Engineering and Regenerative Medicine Applications

Extracellular vesicle-based Nanotherapeutics: Emerging frontiers in anti-inflammatory therapy

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