Injectable Gel Graft for Bone Defect Repair

Fang, Josephine Y.; Yang, Zhi; Tan, ShihJye; Tayag, Charisse; Nimni, Marcel E.; Urata, Mark M.; Han, Bo

doi:10.2217/rme.13.76

Cited by 17 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, massive use could cause hematoma, ectopic bone formation, tumor and excessive ossification [30]. Josephine Fang [31] substantiated that less than 1 mg of BMP-2 was sufficient to promote bone repair. Based on previous studies, 1 mg of BMP-2 was used in the 50 ml thermo-sensitive hydrogel in our study.…”

Section: Joint Histological Analysismentioning

confidence: 99%

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

Intra-articular injection of anti-inflammatory drugs can be a promising strategy for recovery of injured articular cartilage. We prepared a series of injectable thermo-sensitive composite hydrogels, composed of Pluronic F127, glycosaminoglycan (GAG) and bone morphogenetic protein (BMP-2), which was designed to mimic extracellular matrix (ECM). The rheological properties and dissolution rate of composite hydrogels containing chondroitin sulfate or with different hyaluronic acid molecular mass (10k, 90k, 800k) were investigated. Meanwhile, bovine serum albumin (BSA) or FITC-BSA was chosen as model drug loaded into PF/GAG hydrogels to study their sustained release behavior in vitro. The results showed that hydrogels could maintain shapes for more than 16 days and the release rate of BSA in PF/GAG composite gels was much slower than in PF127 gels, due to the affinity between BSA and GAG. Furthermore, increasing the molecular weight of hyaluronic acid correspondingly increased hydrogel dissolution rate and BSA release in the hydrogels. Subsequently, MTT experiments were performed to investigate the toxicity of the hydrogels on mouse pre-osteoblast cell MC3T3-E1. In vivo anti-inflammation results showed that PF/GAG@BMP-2 composite hydrogels had the most efficient efficacy on recovery of injured cartilage, which is induced by osteoarthritis, compared to the control groups (PF127@BMP-2 or BMP-2 saline solution).

show abstract

Section: Joint Histological Analysismentioning

confidence: 99%

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Cao

Han

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

View full text Add to dashboard Cite

show abstract

“…Various tissue engineering applications have already used different hydrogels such as hyaluronic acid, gelatin, chondroitin sulfate, and alginate due to their high water binding capacity, porous structure, and tunable degradation properties . Furthermore, hydrogels have a great potential to be shaped into various morphologies using wide range of fabrication techniques such as 3D printing, wet spinning, and weaving .…”

Section: Introductionmentioning

confidence: 99%

Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration

Celikkin

Mastrogiacomo

Jaroszewicz

et al. 2017

J Biomedical Materials Res

148

110

View full text Add to dashboard Cite

Gelatin methacrylate (GelMA) is an inexpensive, photocrosslinkable, cell-responsive hydrogel which has drawn attention for a wide range of tissue engineering applications. The potential of GelMA scaffolds was demonstrated to be tunable for different tissue engineering (TE) applications through modifying the polymer concentration, methacrylation degree, or UV light intensity. Despite the promising results of GelMA hydrogels in tissue engineering, the influence of polymer concentration for bone tissue engineering (BTE) scaffolds was not established yet. Thus, in this study, we have demonstrated the effect of polymer concentration in GelMA scaffolds on osteogenic differentiation. We prepared GelMA scaffolds with 5 and 10% polymer concentrations and characterized the scaffolds in terms of porosity, pore size, swelling characteristics, and mechanical properties. Subsequent to the scaffolds characterization, the scaffolds were seeded with bone marrow derived rat mesenchymal stem cells and cultured in osteogenic media to evaluate the possible osteogenic differentiation effect exerted by the polymer concentration. After 7, 14, 21, and 28 days, DNA content, calcium deposition, and alkaline phosphatase (ALP) activity of scaffolds were evaluated quantitatively by colorimetric bioassays. Furthermore, the distribution of the calcium deposition within the scaffolds was attained qualitatively and quantitatively by microcomputer tomography (mCT). Our data suggest that GelMA hydrogels prepared with 5% polymer concentration has promoted homogeneous extracellular matrix calcification and it is a great candidate for BTE applications.

show abstract

“…These seed cells can continue to proliferate in vivo and secret growth factors to promote fracture healing [ 21 ]. Among these osteogenic factors, BMP2 has the most significant effect on the bone regeneration [ 22 – 24 ]. Therefore, we used an adenovirus vector carrying BMP2 gene to infect BMSCs in order to achieve a sustained localized expression of bone morphogenetic protein to accelerate fracture healing.…”

Section: Discussionmentioning

confidence: 99%

Acceleration of bone-defect repair by using A-W MGC loaded with BMP2 and triple point-mutant HIF1α-expressing BMSCs

Gao

Wang

et al. 2015

J Orthop Surg Res

View full text Add to dashboard Cite

BackgroundThe goal of this study is to explore the effects of A-W MGC (apatite-wollastonite magnetic bioactive glass-ceramic) loaded with BMP2 (bone morphogenetic protein 2)- and HIF1αmu (hypoxia-inducible factor 1 mutation)-expressing BMSCs (bone marrow mesenchymal stem cells) on the bone defect repair.Methods(1) BMSCs were infected with viral solution containing BMP2 and HIF1αmu with the best MOI (multiplicity of infection). The efficiency was observed via hrGFP (human renilla reniformis green fluorescent protein). (2) The cells were divided into five groups (A–E), and ALP (alkaline phosphatase) activity was measured. (3) BMP2 and HIF1α (hypoxia-inducible factor 1α) protein were measured. (4) A-W MGC was loaded with BMSCs that contain the genes and implanted into the bone defect model. The animals were sacrificed 8 and 12 weeks later. (5) The healing was measured with X-ray, histology, and biomechanics.Results(1) BMSCs in A–D showed high transfection efficiency. (2) ALP in A and B was higher than the others (p = 0.041 or 0.038); A was higher than B (p = 0.038); (3) BMP2 in A and B was higher than the others (p = 0.014). HIF1α in A and C was higher than the others (p = 0.020). (4) 8 and 12 weeks after, an X-ray indicated that bone defect was nearly fully repaired in A and C. (5) 12 weeks after, the bone remodeling was complete in A and C. (6) The flexural strength in A and C was stronger than the others (p = 0.043).ConclusionEngineered A-W MGC with BMP2 and HIF1αmu-expressing BMSCs exhibits comparable therapeutic effects of bone-defect repair as an autologous bone graft.

show abstract

Injectable Gel Graft for Bone Defect Repair

Cited by 17 publications

References 37 publications

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

ECM based injectable thermo-sensitive hydrogel on the recovery of injured cartilage induced by osteoarthritis

Gelatin methacrylate scaffold for bone tissue engineering: The influence of polymer concentration

Acceleration of bone-defect repair by using A-W MGC loaded with BMP2 and triple point-mutant HIF1α-expressing BMSCs

Contact Info

Product

Resources

About