Injectable Hydrogel Containing Tauroursodeoxycholic Acid for Anti-neuroinflammatory Therapy After Spinal Cord Injury in Rats

Han, Gong Ho; Kim, Seong Jun; Ko, Wan‐Kyu; Lee, Daye; Lee, Jae Seo; Nah, Haram; Han, Inbo; Sohn, Seil

doi:10.1007/s12035-020-02010-4

Cited by 25 publications

(36 citation statements)

References 54 publications

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“…Hydrogels acting on ECM can treat SCI by promoting ECM remodeling and eliminating cystic cavity [ 28 , 29 ]. TUDCA hydrogels are used to treat SCI through anti-inflammatory activity [ 30 ]. Compared with the two injectable hydrogels mentioned above, peptide-modified hydrogels and self-assembling hydrogels have certain advantages.…”

Section: Discussionmentioning

confidence: 99%

“…TUDCA is a hydrophilic acid that protects cells. A recent study has shown that TUDCA can be used as a replaceable drug based on its anti-neuro inflammatory effect in SCI rats [ 30 ]. However, the treatment effects were limited because of the use of intraperitoneal injections.…”

Section: Injectable Hydrogels Therapymentioning

confidence: 99%

“…Hydrogels, a high-water-content material, have been proposed as a drug delivery system that effectively offers a drug at a suitable targeted site in a short period. A research has found that direct injection of the TUDCA-hydrogel (TC gel) can decrease the amount of glial fibrillary acidic protein (GFAP) which is a marker of activated astrocyte to inhibit the inflammatory effect and promote functional recovery after SCI [ 30 ]. Moreover, hydrogels have no side effects, so the anti-inflammatory effect of TUDCA can be better developed.…”

Section: Injectable Hydrogels Therapymentioning

confidence: 99%

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Multimodal therapy strategies based on hydrogels for the repair of spinal cord injury

Wang

Chao

et al. 2022

Military Med Res

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Spinal cord injury (SCI) is a serious traumatic disease of the central nervous system, which can give rise to the loss of motor and sensory function. Due to its complex pathological mechanism, the treatment of this disease still faces a huge challenge. Hydrogels with good biocompatibility and biodegradability can well imitate the extracellular matrix in the microenvironment of spinal cord. Hydrogels have been regarded as promising SCI repair material in recent years and continuous studies have confirmed that hydrogel-based therapy can effectively eliminate inflammation and promote spinal cord repair and regeneration to improve SCI. In this review, hydrogel-based multimodal therapeutic strategies to repair SCI are provided, and a combination of hydrogel scaffolds and other therapeutic modalities are discussed, with particular emphasis on the repair mechanism of SCI.

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Section: Discussionmentioning

confidence: 99%

Section: Injectable Hydrogels Therapymentioning

confidence: 99%

Section: Injectable Hydrogels Therapymentioning

confidence: 99%

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Multimodal therapy strategies based on hydrogels for the repair of spinal cord injury

Wang

Chao

et al. 2022

Military Med Res

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“…The BMMs were differentiated into osteoclasts with CCM containing two concentrations (0.01 and 0.05 nM) of GNPs and SNPs. Cells were lysed with cold RIPA buffer containing protease (Roche Applied Science, Indianapolis, IN, USA) and phosphatase inhibitor cocktails (Sigma, St. Louis, MO, USA) [20,24]. The cells lysates were incubated on ice for 5 min and then centrifuged (10 min, 13,000× g).…”

Section: Western Blotmentioning

confidence: 99%

Inhibitory Effects of Gold and Silver Nanoparticles on the Differentiation into Osteoclasts In Vitro

Lee

Kim

et al. 2021

Pharmaceutics

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Gold nanoparticles (GNPs) have been widely studied to inhibit differentiation into osteoclasts. However, reports of the inhibitory effects of silver nanoparticles (SNPs) during the process of differentiation into osteoclasts are rare. We compared the inhibitory effect of GNPs and SNPs during the process of differentiation into osteoclasts. Bone marrow-derived cells were differentiated into osteoclasts by the receptor activator of the nuclear factor-kappa-Β ligand (RANKL). The inhibitory effect of GNPs or SNPs during the process of differentiation into osteoclasts was investigated using tartrate-resistant acid phosphatase (TRAP) and actin ring staining. The formation of TRAP positive (+) multinuclear cells (MNCs) with the actin ring structure was most inhibited in the SNP group. In addition, the expression of specific genes related to the differentiation into osteoclasts, such as c-Fos, the nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), TRAP, and Cathepsin K (CTSK) were also inhibited in the SNP groups. As a result, the levels related to differentiation into osteoclasts were consistently lower in the SNP groups than in the GNP groups. Our study suggests that SNPs can be a useful material for inhibiting differentiation into osteoclasts and they can be applied to treatments for osteoporosis patients.

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“…The detailed procedures for Western blot are described in our previous work [27]. BMDMs were seeded on 6 well culture plate (Falcon, 1 × 10 6 cells/well, n = 3 per group) and treated with indicated concentrations of LOBE and LPS for 24 h. At 24 h, the total proteins were extracted using ice-cold RIPA lysis buffer with protease inhibitor (Roche Applied Science, Indianapolis, IN, USA) and phosphatase inhibitor cocktails (Sigma).…”

Section: Western Blotmentioning

confidence: 99%

Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages

Jeong

Kim

et al. 2021

Biomedicines

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The purpose of this study is to elucidate the anti-inflammatory effect of lobeglitazone (LOBE) in lipopolysaccharide (LPS)-induced bone-marrow derived macrophages (BMDMs). We induced nitric oxide (NO) production and pro-inflammatory gene expression through LPS treatment in BMDMs. The changes of NO release and expression of pro-inflammatory mediators by LOBE were assessed via NO quantification assay and a real-time quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the regulatory effect of LOBE on activation of mitogen-activated protein kinase (MAPK) signaling pathway was investigated by measuring the phosphorylation state of extracellular regulatory protein (ERK) and c-Jun N-terminal kinase (JNK) proteins by Western blot. Our results show that LOBE significantly reduced LPS-induced NO production and pro-inflammatory gene expression of interleukin-1β (IL-1β), IL-6, inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and monocyte chemoattractant protein-1 (MCP-1). Moreover, LOBE reduced phosphorylation levels of ERK and JNK of MAPK signaling pathway. In conclusion, LOBE exerts an anti-inflammatory effect in LPS-induced BMDMs by suppression of NO production and pro-inflammatory gene expression, and this effect is potentially through inhibition of the MARK signaling pathway.

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Injectable Hydrogel Containing Tauroursodeoxycholic Acid for Anti-neuroinflammatory Therapy After Spinal Cord Injury in Rats

Cited by 25 publications

References 54 publications

Multimodal therapy strategies based on hydrogels for the repair of spinal cord injury

Multimodal therapy strategies based on hydrogels for the repair of spinal cord injury

Inhibitory Effects of Gold and Silver Nanoparticles on the Differentiation into Osteoclasts In Vitro

Lobeglitazone Exerts Anti-Inflammatory Effect in Lipopolysaccharide-Induced Bone-Marrow Derived Macrophages

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