Injectable PEG-induced silk nanofiber hydrogel for vancomycin delivery

Sun, Yusheng; Zhang, Peng; Zhang, Feng; Pu, Mengyang; Zhong, Wentao; Zhang, Yu; Shen, Yingchao; Zuo, Baoqi

doi:10.1016/j.jddst.2022.103596

Cited by 8 publications

(9 citation statements)

References 46 publications

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“…Similar to the methods observed in previous research, degummed silk was dissolved in a CaCl 2 /FA dissolution system, air-dried, and then dissolved into a silk fibroin membrane with a CaCl 2 /ethanol/H 2 O ternary dissolution system to obtain the SF–NF. It was found that the crystal structure of the SF–NF is mainly a typical transition state silk I structure, which easily changes into a stable silk II structure under external stimulation and induction, thus giving the SF–NF the ability to quickly gel and be injected ( LU et al, 2010 ; SUN et al, 2022 ).…”

Section: Resultsmentioning

confidence: 99%

“…Through a preliminary study in the laboratory, it was found that the CaCl 2 /formic acid (Ca–FA) dissolution system could decompose silk fibroin into a nanofiber and further prepare a hydrogel with the morphology of an extracellular matrix (ECM) fiber network to simulate the ECM environment ( MING et al, 2014 ). SUN et al (2022) dissolved silk fibroin in the Ca/FA dissolution system and obtained an aqueous silk fibroin nanofiber (SF-NF) solution. An injectable SF–NF hydrogel with a faster gel rate and longer vancomycin sustained-release period was further prepared.…”

Section: Introductionmentioning

confidence: 99%

“…In this study, silk fibroin microspheres (SFMPs) were used as drug carriers to load vancomycin (VANCO) to form drug microspheres (VSFMPs), and then a silk fibroin nanofiber (SF–NF) solution was used to prepare an injectable hydrogel as a carrier for VSFMPs (SF-NF@VSFMPs), which forms a modified delivery system for chronic osteomyelitis. SF-NF was obtained by sequentially dissolving silk fibroin in two solutions: CaCl 2 /FA and CaCl 2 /C 2 H 5 OH/H 2 O ( SUN et al, 2022 ). The injectable hydrogel formed by blending the SF-NF with low-molecular weight polyethylene glycol (PEG400) has good injectable properties and stability ( WANG et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy

Zhang

Sun

Yang

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: Chronic osteomyelitis remains a clinical challenge in orthopedics.Methods: In this study, silk fibroin microspheres (SFMPs) loaded with vancomycin are entrapped in an injectable silk hydrogel to form a vancomycin delivery system for treatment of chronic osteomyelitis.Results and Discussion: Vancomycin showed continuous release from the hydrogel for up to 25 days. The hydrogel shows strong antibacterial activity against both Escherichia coli and Staphylococcus aureus and a long antibacterial duration of 10 days without a decrease in the antibacterial effect. The injection of vancomycin-loaded silk fibroin microspheres entrapped in the hydrogel into the infected site of rat tibia reduced bone infection and improved bone regeneration compared with other treatment groups.Conclusion: Thus, the composite SF hydrogel features a sustained-release profile and good biocompatibility, making it promising for application in osteomyelitis treatment.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy

Zhang

Sun

Yang

et al. 2023

Front. Bioeng. Biotechnol.

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“…Particularly, the group with a PPG content of 2.8 wt% demonstrated an obvious yield strain after reaching the yield point of 25% strain. 28 Although pure silk hydrogel possesses considerable strength, its elongation at break and toughness are limited, thereby restricting its further application. However, with the addition of PPG, the elongation at break, toughness, and strength all have been improved 2, it can be observed that the hydrogel exhibits optimal mechanical properties when the PPG content is 2.8 wt%.…”

Section: Rresults and Discussionmentioning

confidence: 99%

Tough silk fibroin hydrogel via polypropylene glycol (PPG) blending for wearable sensors

Xiao,

Wu,

et al. 2023

J of Applied Polymer Sci

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Regenerated silk fibroin is a promising bio‐material because of its sustainability, biocompatibility, degradability, and eco‐friendliness. However, silk fibroin hydrogel's intrinsic hard and brittle mechanical properties hinder its application on human skin as a wearable sensor. We used polypropylene glycol (PPG) as a plasticizer to physically blend with a silk fibroin solution, and designed a set of green and environmentally friendly preparation methods without organic solvents to obtain the hydrogel for wearable sensors. PPG molecules will aggregate into clusters to act as a plasticizer. Meanwhile, a large number of hydrogen bonds are formed between the hydroxyl groups in PPG and silk fibroin molecular chains. When the hydrogel is stretched, the hydrogen bond in the silk fibroin chain will release heat in an irreversible form to increase the toughness. The PPG/silk fibroin hydrogel exhibits mechanical strength of 16.6 MPa, breaking elongation of 160%, and toughness of 17.7 MJ/m3. The Ca2+ introduced during the sample preparation process gives the hydrogel ionic conductivity, which provides the possibility of using the hydrogel as a human skin sensing material.

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“…[ 14 ] In another study, a VAN‐loaded injectable nanofibrous hydrogel patch showed good antimicrobial and biocompatible properties. [ 15 ] In the study by Riau et al., VAN‐loaded collagen hydrogel exhibited a good drug release profile. The hydrogel was implanted in vivo in a rabbit model with keratitis and reduced the infection.…”

Section: Introductionmentioning

confidence: 99%

A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug‐Loaded Nanofibrous Patches

Cesur

Ilhan

Pilavci

et al. 2023

Macro Materials & Eng

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Corneal ulcer, which is brought on by a breach in the epithelial barrier, is a dangerous infection of the avascular corneal stroma. New treatment strategies are needed, suppressing the aggressive nature of the disease and including a combination of different drugs. In this study, vancomycin (VAN) and fluconazole (FLU) dual‐drug loaded dual‐layered polyvinyl alcohol and gelatin (PVA/GEL) nanofibrous patches are produced by electrospinning. Scanning electron microscopy (SEM) images show smooth surfaces are obtained for both pure and drug‐loaded nanofibrous patches. The tensile test results report that loading the FLU and VAN separately into the PVA/GEL patches decrease both the tensile strength and elongation at break and it is further reduced when combining two drug‐loaded layers in one patch. According to drug release results, the FLU and VAN‐loaded nanofibrous patches show a controlled release profile extending up to 96 h. Moreover, PVA/GEL/FLU, PVA/GEL/VAN, and PVA/GEL/FLU/VAN nanofibrous patches display significant antimicrobial activity against Candida albicans and Staphylococcus aureus. SEM, 4'‐6diamidynofenyloindol (DAPI) staining, and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay show that PVA/FLU and PVA/GEL/FLU/VAN nanofibrous patches have a superior effect on NIH3T3 cell spreading and proliferation. The novelty of this study lays in the development of a potential dual drug rapid treatment for corneal ulcers of aggressive nature.

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Injectable PEG-induced silk nanofiber hydrogel for vancomycin delivery

Cited by 8 publications

References 46 publications

Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy

Vancomycin-loaded silk fibroin microspheres in an injectable hydrogel for chronic osteomyelitis therapy

Tough silk fibroin hydrogel via polypropylene glycol (PPG) blending for wearable sensors

A Novel Strategy as a Potential Rapid Therapy Modality in the Treatment of Corneal Ulcers: Fluconazole/Vancomycin Dual Drug‐Loaded Nanofibrous Patches

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