Injectable thermosensitive hydrogel to modulate tolerogenic dendritic cells under hyperglycemic condition

Abstract

Scaffolds and hydrogels can deliver therapeutic or immunomodulatory agents to regulate the bone healing in periodontal inflammation. Hyperglycemia associated with diabetes mellitus is a significant risk factor for periodontitis as...

Injectable smart stimuli-responsive hydrogels: pioneering advancements in biomedical applications

“…71 Similarly, Zhu et al prepared a thermosensitive injectable self-assembled hydrogel (TISH) using chitosan which could transition from liquid phase to gelation phase upon incubation at 37 °C. 78 This hydrogel was loaded with resveratrol and granulocyte-macrophage colony-stimulating factor (GM-CSF) to release the bioactive molecules to induce tolerogenic dendritic cells and regulatory T-cells, and finally attenuate diabetic periodontitis.…”

Rational Design of Bioactive Hydrogels toward Periodontal Delivery: From Pathophysiology to Therapeutic Applications

“…designed a thermosensitive injectable chitosan‐based hydrogel for controlled and sustained delivery of GM‐CSF and resveratrol to modulate DCs toward a tolerogenic phenotype. [ 174 ] DCs showed increased tolerogenic genes (e.g., FOXP3 , SOCS3 , TGF‐β , IL‐10 ) and decreased maturation and activation genes (e.g., CD80 , CD83 , CD86 ) when cultured with the hydrogel in vitro under hyperglycemic condition. The co‐culture experiment found that tolerogenic DCs could promote the differentiation of naive T cells to regulatory T cells.…”

“…Anti-inflammatory [174] Figure 6. The anti-inflammatory effects of the chitosan/β-GP/gelatin-based hydrogel loaded with aspirin and erythropoietin.…”

“…It is emphasized that various components of innate responses (e.g., neutrophils, macrophages, dendritic cells, complements) play both inductive and regulatory roles in host responses. [29] Guided by the latest knowledge regarding the cellular and molecular mechanisms underlying periodontitis, researchers have designed advanced functional hydrogel delivery systems for periodontitis treatment through targeting conversion of macrophage phenotypes, [69,85,86,89,97] induction of tolerogenic DCs, [77,174] and more recently, inactivation of complement C5a receptors (C5aR). [102,175] Macrophages are important innate immune cells that turn to the pro-inflammatory phenotype (M1) when activated by bacteria and produce lots of cytokines triggering inflammatory cascades, such as IL-1β and TNF-α.…”

A whole-course-repair system based on ROS/glucose stimuli-responsive EGCG release and tunable mechanical property for efficient treatment of chronic periodontitis in diabetic rats

“…Firstly, some researchers have emphasized the control of tissue inflammation and immunomodulation, potentially neglecting the fact that the ultimate aim of periodontal therapy is to promote the regeneration of periodontal tissue. [17][18][19] Secondly, traditional hydrogels often exhibit suboptimal biomechanical properties, making them susceptible to damage from external forces when applied to periodontal tissue. 20,21 In recent years, incorporating nanosized particles or other bioactive materials, as well as increasing the hydrogel polymer concentration or crosslink density, have been commonly employed as major strategies to enhance mechanical property.…”