Injury‐induced rapid activation of MAPK signaling in dechorionated eggs and larvae of the silkworm <i>Bombyx mori</i>

Gu, Shi‐Hong; Chen, Chien‐Hung

doi:10.1111/1744-7917.12301

Cited by 12 publications

(5 citation statements)

References 40 publications

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“…In the present study, we found that dechorionation significantly increased insulin, and other growth factors (Buchon et al, 2014). Injury-induced rapid activation of MAPK signaling in dechorionated eggs and larvae of B. mori was also previously reported (Gu & Chen, 2017).…”

Section: Changes In Gene Expressions and Ecdysteroid Levels In Dechor...supporting

confidence: 88%

“…In Drosophila , it is well documented that upon damage, intestinal regeneration is mediated by numerous factors including cytokines, insulin, and other growth factors (Buchon et al, 2014). Injury‐induced rapid activation of MAPK signaling in dechorionated eggs and larvae of B. mori was also previously reported (Gu & Chen, 2017). In addition, a previous study showed that U0126 at 100 µM inhibited EPPase gene expressions in dechorionated and incubated eggs (Fujiwara, Tanaka et al, 2006).…”

Section: Discussionsupporting

confidence: 76%

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Expressions of sugar transporter genes during Bombyx mori embryonic development

Lin

Chang

2023

J Exp Zool Pt A

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Sugar transporters (Sts) play important roles in controlling carbohydrate transport and are responsible for mediating the movement of sugars into cells. Few studies have been conducted on expressions of Sts during insect embryonic development. In the present study, we investigated temporal expressions of St genes during the embryonic diapause process in Bombyx mori. We found that in HCl‐treated developing eggs, high gene expressions of trehalose transporter 1 (Tret1) were detected during middle and later embryonic development. St4 and St3 gene expressions gradually increased during the early stages, reached a small peak on Day 3, and large peaks were again detected on Day 7. However, in diapause eggs, expression levels of the Tret1, St4, and St3 genes all remained at low levels. Differential temporal changes in expressions of the Tret1, St4, and St3 genes found between diapause and HCl‐treated eggs were further confirmed using nondiapause eggs. Our results showed that nondiapause eggs exhibited similar changing patterns as those of HCl‐treated eggs, thus clearly indicating potential correlations between expressions of these genes and embryonic development. In addition, high gene expressions of Tret1 were also detected when dechorionated eggs were incubated in the medium. The addition of LY294002 (a specific phosphatidylinositol 3‐kinase [PI3K] inhibitor) and U0126 (a mitogen‐activated protein kinase/extracellular signal‐regulated kinase [ERK] kinase [MEK] inhibitor) partially inhibited Tret1 gene expression in dechorionated eggs, but did not affect either ecdysteroid‐phosphate phosphatase gene expression or ecdysteroid biosynthesis, clearly indicating that both PI3K and ERK are involved in increased gene expression of Tret1 that was independent of ecdysteroid levels. To our knowledge, this is the first comprehensive report to demonstrate the transcriptional regulation of St genes during embryonic development, thus providing useful information for a clearer understanding of insect egg diapause mechanisms.

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Section: Changes In Gene Expressions and Ecdysteroid Levels In Dechor...supporting

confidence: 88%

Section: Discussionsupporting

confidence: 76%

Expressions of sugar transporter genes during Bombyx mori embryonic development

Lin

Chang

2023

J Exp Zool Pt A

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“…ERK is involved in important physiological processes in insects, including cell proliferation, differentiation, and resistance to apoptosis (Bonnefoy‐Berard et al, 2004), and is therefore essential in insect growth and development. It also regulates a range of physiological functions, such as growth and development (Fujiwara, Shindome, et al, 2006), reproduction (Guo et al, 2019), innate immunity (Lin & Gu, 2007), (Fujiwara, Shindome, et al, 2006; Guo et al, 2019; Lin & Gu, 2007), the synthesis of silk proteins (Ma et al, 2018), and diapause of eggs (Gu & Chen, 2017). Notably, the various cascade kinases involved in the activation of ERK also play unique roles in silkworms.…”

Section: Functions Of the Erk Pathway In Silkwormsmentioning

confidence: 99%

“…It also regulates a range of physiological functions, such as growth and development (Fujiwara, Shindome, et al, 2006), reproduction (Guo et al, 2019), innate immunity (Lin & Gu, 2007), (Fujiwara, Shindome, et al, 2006;Guo et al, 2019;Lin & Gu, 2007), the synthesis of silk proteins (Ma et al, 2018), and diapause of eggs (Gu & Chen, 2017). Notably, the various cascade kinases involved in the activation of ERK also play unique roles in silkworms.…”

Section: Functions Of the Erk Pathway In Silkwormsmentioning

confidence: 99%

Advances in the extracellular signal‐regulated kinase signaling pathway in silkworms, Bombyx mori (Lepidoptera)

Lv,

Zheng,

Wang

et al. 2023

Arch Insect Biochem Physiol

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Signaling pathways regulate the transmission of signals during organism growth and development, promoting the smooth and accurate completion of numerous physiological and biochemical reactions. The extracellular signal‐regulated kinase (ERK) signaling pathway is an essential pathway involved in regulating various physiological processes, such as cell proliferation, differentiation, adhesion, migration, and more. This pathway also contributes to several important physiological processes in silkworms, including protein synthesis, reproduction, and immune defense against pathogens. Organizing related studies on the ERK signaling pathway in silkworms can provide a better understanding of its mechanism in Lepidopterans and develop a theoretical foundation for improving cocoon production and new strategies for pest biological control.

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“…Both the ERK- and JNK-dependent signaling pathways appear to contribute to Bombyx nucleopolyhedrovirus infection (Katsuma et al, 2007 ). More recently, we reported that JNK signaling together with other MAPK signaling pathways, which are rapidly induced by injury, are related to diapause termination in dechorionated Bombyx eggs (Gu and Chen, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

Hsieh

et al. 2018

Front. Physiol.

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In this study, phosphorylation of c-Jun N-terminal kinase (JNK) by the prothoracicotropic hormone (PTTH) was investigated in prothoracic glands (PGs) of the silkworm, Bombyx mori. Results showed that JNK phosphorylation was stimulated by the PTTH in time- and dose-dependent manners. In vitro activation of JNK phosphorylation in PGs by the PTTH was also confirmed in an in vivo experiment, in which a PTTH injection greatly increased JNK phosphorylation in PGs of day-6 last instar larvae. JNK phosphorylation caused by PTTH stimulation was greatly inhibited by U73122, a potent and specific inhibitor of phospholipase C (PLC) and an increase in JNK phosphorylation was also detected when PGs were treated with agents (either A23187 or thapsigargin) that directly elevated the intracellular Ca2+ concentration, thereby indicating involvement of PLC and Ca2+. Pretreatment with an inhibitor (U0126) of mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) kinase (MEK) and an inhibitor (LY294002) of phosphoinositide 3-kinase (PI3K) failed to significantly inhibit PTTH-stimulated JNK phosphorylation, indicating that ERK and PI3K were not related to JNK. We further investigated the effect of modulation of the redox state on JNK phosphorylation. In the presence of either an antioxidant (N-acetylcysteine, NAC) or diphenylene iodonium (DPI), PTTH-stimulated JNK phosphorylation was blocked. The JNK kinase inhibitor, SP600125, markedly inhibited PTTH-stimulated JNK phosphorylation and ecdysteroid synthesis. The kinase assay of JNK in PGs confirmed its stimulation by PTTH and inhibition by SP600125. Moreover, PTTH treatment did not affect JNK or Jun mRNA expressions. Based on these findings, we concluded that PTTH stimulates JNK phosphorylation in Ca2+- and PLC-dependent manners and that the redox-regulated JNK signaling pathway is involved in PTTH-stimulated ecdysteroid synthesis in B. mori PGs.

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Injury‐induced rapid activation of MAPK signaling in dechorionated eggs and larvae of the silkworm Bombyx mori

Cited by 12 publications

References 40 publications

Expressions of sugar transporter genes during Bombyx mori embryonic development

Expressions of sugar transporter genes during Bombyx mori embryonic development

Advances in the extracellular signal‐regulated kinase signaling pathway in silkworms, Bombyx mori (Lepidoptera)

Stimulation of JNK Phosphorylation by the PTTH in Prothoracic Glands of the Silkworm, Bombyx mori

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