Ink-jet printing an optimal multi-enzyme system

Zhang, Yifei; Lyu, Fengjiao; Ge, Jun; Liu, Zheng

doi:10.1039/c4cc06158f

Cited by 71 publications

(54 citation statements)

References 25 publications

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“…The detection range of the testing strips is from 20 to 120 mM, with a limit of 20 mM by visible detection, making it promising for fast and convenient determination of H 2 O 2 in solution. Detection of H 2 O 2 is important in food, pharmaceutical, textile industries, and environmental protection (Zhang et al 2014b;Jv et al 2010). As an additive for microbial inhibiting or bleaching, H 2 O 2 has been used in food industry (Nascimento et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

Hou

Zhao²,

Feng

et al. 2017

Bioresour. Bioprocess.

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Objectives: This report explores the possibility of synthesizing enzyme-metal-organic framework (MOF) composites by ink-jet printing.Results: This study demonstrates that the direct synthesis of patterned enzyme-metal-organic framework (MOF) composites on various substrates including paper and polymeric films can be readily achieved by ink-jet printing bio-inks containing protein molecules, metal ions, and organic ligands loaded, respectively, in different cartridges. The formed Cytochrome c (Cyt c)-MOF composites on filter paper by ink-jet printing can be used for rapid detection of hydrogen peroxide in solution. Conclusions:This technique opens possibilities of scalable, controllable, and designable fabrication of functional protein-MOF hybrid surface with promising applications in future bio-related application fields such as biosensing, wearable bioelectronics, artificial biomimetic membranes, and tissue engineering.

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Section: Resultsmentioning

confidence: 99%

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

Hou

Zhao²,

Feng

et al. 2017

Bioresour. Bioprocess.

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“…[121] In this study, two sets of enzyme combinations (GOx/HRP, diaphrose (DP)/alcohol dehydrogenase (ADH)) were printed in different proportions to achieve the most effective bienzymatic reactions. After proving a good agreement between the preset mass ratio (CMYK color value in the graphic software) and the printed mass ratio (judged from the emission of fluorescently labeled enzymes), the enzyme ratios to obtain maximum reaction yields (GOx/ HRP = 2:8, DP/ADH = 5:95) were determined (the case of GOx/HRP is shown in Figure 16).…”

Section: Simultaneous Printing Of Multiple Reagentsmentioning

confidence: 99%

Paper‐Based Inkjet‐Printed Microfluidic Analytical Devices

et al. 2015

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Rapid, precise, and reproducible deposition of a broad variety of functional materials, including analytical assay reagents and biomolecules, has made inkjet printing an effective tool for the fabrication of microanalytical devices. A ubiquitous office device as simple as a standard desktop printer with its multiple ink cartridges can be used for this purpose. This Review discusses the combination of inkjet printing technology with paper as a printing substrate for the fabrication of microfluidic paper-based analytical devices (μPADs), which have developed into a fast-growing new field in analytical chemistry. After introducing the fundamentals of μPADs and inkjet printing, it touches on topics such as the microfluidic patterning of paper, tailored arrangement of materials, and functionalities achievable exclusively by the inkjet deposition of analytical assay components, before concluding with an outlook on future perspectives.

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“…For example Zhang et al. used inks with enzymes, surfactants and PEG 20 000 for optimization‐assays of glucose oxidase/horseradish peroxidase (GOx/HRP) and alcohol dehydrogenase/diaphorase (ADH/DP) ratio gradients. In another modification, inkjet etching was used by Abe et al.…”

Section: Surface Effectsmentioning

confidence: 99%

“…Inks containing enzymes, nanoparticles, chromogenic indicators, antibodies, etc., can be used for the paper modification. For example Zhang et al [64] used inks with enzymes, surfactants and PEG 20 000 for optimization-assays of glucose oxidase/horseradish peroxidase (GOx/HRP) and alcohol dehydrogenase/diaphorase (ADH/DP) ratio gradients. In another modification, inkjet etching was used by Abe et al [65] to expose polystyrene-soaked paper by piezoelectric ejection of toluene and modify the exposed surface for tetrabromophenol-blue-based protein assay.…”

Section: Surface Effectsmentioning

confidence: 99%

Fluid manipulation on the micro‐scale: Basics of fluid behavior in microfluidics

Novotný

Foret

2016

J of Separation Science

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Fluid manipulation on the micro-scale (microfluidics) is bringing new potential applications in a number of fields, including chemistry, biology and medicine. At sub-millimeter channel scale, some phenomena, unimportant at the macroscale, become an important force to consider when designing a microfluidics system. For example, the decrease in fluid mass causes the effects of viscosity to overcome the influence of inertia. Turbulent flow cannot be achieved at any realistic fluid velocity, making mixing a challenging task. The only phenomenon capable of blending liquids at microscale is diffusion and liquid streams can be flowed side-by-side for tens of minutes before they completely fuse together. The decrease in the channel size also leads to an increased surface-to-volume ratio, which increases the importance of surface effects, including adsorption, capillary action and surface wetting and/or electric double layer formation with related electrokinetic phenomena. While rivers cannot flow uphill, a stream of liquid can easily flow up against gravity inside a capillary. Similarly, the formation of electric double layer near the charged surface of a micro-channel or capillary can be applied for electrokinetic actuating. This review summarizes selected physical phenomena related to liquid-based (water solutions) microfluidics as described recently.

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Ink-jet printing an optimal multi-enzyme system

Cited by 71 publications

References 25 publications

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

Paper‐Based Inkjet‐Printed Microfluidic Analytical Devices

Fluid manipulation on the micro‐scale: Basics of fluid behavior in microfluidics

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