Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts

Talayero, Paloma; Mancebo, Esther; Pulido, Jorge Calvo; Rodríguez-Muñoz, S; Bernardo, Iván; Laguna‐Goya, Rocío; Cano-Romero, Francisco; García-Sesma, Álvaro; Loinaz, Carmelo; Jiménez, Carlos; Alonso, Iago Justo; Paz‐Artal, Estela

doi:10.1111/ajt.13435

Cited by 38 publications

(26 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we first recorded that at day 14 after transplantation, Lin + and NK cells were diminished whereas circulatory hILC numbers were similar to CS. This observation is consistent with our previous study in intestinal transplantation where we described that ILC persisted whereas T cells were depleted in the epithelium of intestinal grafts during the first 2 years posTx [6]. Moreover, by comparing circulatory cell counts at preTx and day 14 posTx, here we showed that NK cell numbers decreased while hILC subsets remained unchanged.…”

Section: Discussionsupporting

confidence: 93%

See 1 more Smart Citation

Number and function of circulatory helper innate lymphoid cells are unaffected by immunosuppressive drugs used in solid organ recipients – a single centre cohort study

et al. 2020

View full text Add to dashboard Cite

Summary In transplanted intestines, depletion of T cells together with long‐term persistence of ILC is observed, suggesting ILC insensitivity to immunosuppressive drugs. To further analyze helper ILC (hILC) apparent resistance to therapy, cytotoxic ILC (NK cells), hILC subsets (ILC1, ILC2, and ILC precursors (ILCP)), and their signature cytokines (IFNγ, IL4 + IL13, and IL22) were analyzed in peripheral blood of kidney and liver transplant recipients. Early after transplantation (posTx), transplanted patients showed significantly lower Lin + and NK cells, whereas total hILC, ILC1, ILC2, and ILCP numbers were similar in patients and controls. Between paired pre‐ and posTx samples, Lin + cell and NK cell counts significantly decreased, whereas all three hILC counts and their cytokine production remained similar. ILC1, ILC2, and ILCP numbers were also similar in patients under thymoglobulin or basiliximab (BAS), patients without induction (only maintenance therapy) and controls. hILC showed lower TMG binding comparing to Lin + cells, reduced expression of CD25 (BAS target), and diminished calcineurin activity with undetectable calcineurin and FKBP12 (tacrolimus target). hILC counts were not related to delayed graft function or biopsy‐proven acute rejection. Thus, hILC remain stable early after transplantation and seem unaffected by immunosuppressors, which may be related to reduced targets expression and low calcineurin activity.

show abstract

Section: Discussionsupporting

confidence: 93%

“…We and others have found that ILC are present in transplanted intestines and survive for long periods of time [6,7]. Moreover, during the two first years after transplantation, ILC become the most prominent lymphoid population in intestinal mucosa while a profound depletion of T cells is observed [5].…”

Section: Introductionmentioning

confidence: 76%

Number and function of circulatory helper innate lymphoid cells are unaffected by immunosuppressive drugs used in solid organ recipients – a single centre cohort study

et al. 2020

View full text Add to dashboard Cite

show abstract

“…In accordance with the accumulation of neutrophils in psoriasis, the gene expression of neutrophilic cytochemokines CXCL1, CXCL2, CXCL8, and IL-36 is upregulated in the lesional skin of psoriasis [68,[155][156][157]. Psoriatic keratinocytes produce a large amount of CCL20 [158,159], which is a potent chemoattractant for CCR6 + IL-17A-producing Th17 [89,143,160], ILC3 [161,162], Tc17 [163,164], and γδ T cells [78,165]. Interestingly, IL-17A actively upregulates the production of these key cytochemokines as well as proliferative capacity in keratinocytes.…”

Section: Il-17a and Psoriasismentioning

confidence: 93%

Interleukin-17A and Keratinocytes in Psoriasis

Furue

Tsuji

et al. 2020

IJMS

190

155

View full text Add to dashboard Cite

The excellent clinical efficacy of anti-interleukin 17A (IL-17A) biologics on psoriasis indicates a crucial pathogenic role of IL-17A in this autoinflammatory skin disease. IL-17A accelerates the proliferation of epidermal keratinocytes. Keratinocytes produce a myriad of antimicrobial peptides and chemokines, such as CXCL1, CXCL2, CXCL8, and CCL20. Antimicrobial peptides enhance skin inflammation. IL-17A is capable of upregulating the production of these chemokines and antimicrobial peptides in keratinocytes. CXCL1, CXCL2, and CXCL8 recruit neutrophils and CCL20 chemoattracts IL-17A-producing CCR6+ immune cells, which further contributes to forming an IL-17A-rich milieu. This feed-forward pathogenic process results in characteristic histopathological features, such as epidermal hyperproliferation, intraepidermal neutrophilic microabscess, and dermal CCR6+ cell infiltration. In this review, we focus on IL-17A and keratinocyte interaction regarding psoriasis pathogenesis.

show abstract

“…We (Zuber et al, manuscript submitted) and others have shown that donor lymphocytes persist to varying degrees after intestinal and multivisceral transplantation (2–4). A recent report has characterized the phenotypes of innate lymphoid cells (ILCs) found in the gut after transplantation (5). However, currently there is no published information about recipient replacement of donor innate lymphoid cells after transplantation.…”

Section: Introductionmentioning

confidence: 99%

Long-term Persistence of Innate Lymphoid Cells in the Gut After Intestinal Transplantation

et al. 2017

View full text Add to dashboard Cite

Background Little is known about innate lymphoid cell (ILC) populations in the human gut, and the turnover of these cells and their subsets after transplantation has not been described. Methods Intestinal samples were taken from 4 isolated intestine (ITx) and 3 multivisceral (MvTx) transplant recipients at the time of any operative resection, such as stoma closure or revision. ILCs were isolated and analyzed by flow cytometry. The target population was defined as being negative for lineage markers and double-positive for CD45/CD127. Cells were further stained to define ILC subsets and a donor- or recipient-specific HLA marker to analyze chimerism. Results Donor-derived ILCs were found to persist greater than 8 years after transplantation. Additionally, the percentage of cells thought to be Lymphoid tissue inducer (LTi) cells among donor ILCs was far higher than that among recipient ILCs. Conclusions Our findings demonstrate that donor-derived ILCs persist long-term after transplantation and support the notion that human LTi cells may form in the fetus and persist throughout life, as hypothesized in rodents. Correlation between chimerism and rejection, graft failure, and patient survival requires further study.

show abstract

Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts

Abstract: We examined intraepithelial lymphocytes (IELs)

Cited by 38 publications

References 43 publications

Number and function of circulatory helper innate lymphoid cells are unaffected by immunosuppressive drugs used in solid organ recipients – a single centre cohort study

Number and function of circulatory helper innate lymphoid cells are unaffected by immunosuppressive drugs used in solid organ recipients – a single centre cohort study

Interleukin-17A and Keratinocytes in Psoriasis

Long-term Persistence of Innate Lymphoid Cells in the Gut After Intestinal Transplantation

Contact Info

Product

Resources

About