Inner Filter Effect in the Fluorescence Emission Spectra of Room Temperature Ionic Liquids with-β-Carotene

Pawlak, Krzysztof; Skrzypczak, Andrzej; Bialek-Bylka, Grazyna E.

doi:10.5772/20768

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Since both the excitation and emission spectra of the CNDs were affected by the absorption spectra of rifampicin, this means the type of inner filter effect involved both the primary and secondary IFE [50]. Since both the excitation and emission spectra of the CNDs were affected by the absorption spectra of rifampicin, this means the type of inner filter effect involved both the primary and secondary IFE [50].…”

Section: Mechanism Of Quenchingmentioning

confidence: 99%

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

2020

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The determination of rifampicin in pharmaceutical dosage forms using a rapid, sensitive, selective, biocompatible, and low-cost method is of vital importance in the pharmaceutical analysis field to ensure its concentration is within the effective range when administered. In this study, nitrogen-and-phosphorous-doped carbon nanodots (CNDs) were prepared using a single-step hydrothermal method with ciprofloxacin as the starting material. The CNDs showed a highly intense blue fluorescence emission centered at 450 nm, with a photoluminescence quantum yield of about 51%. Since the absorption of rifampicin was the same as the excitation spectrum of CNDs, inner filter effect (IFE) quenching occurred and it was used as a successful detection platform for the analysis of rifampicin in capsules. The detection platform showed a dynamic linear range from 1 to 100 μM (R2 = 0.9940) and the limit of detection was 0.06 μM (when S/N = 3). The average spike recovery percentage for rifampicin in the capsule samples was 100.53% (n = 5). Moreover, the sub-chronic cytotoxicity of CNDs was evaluated on healthy male mice (Balb/c) drenched with different amounts of CNDs (10 and 50 mg/kg). During this study period, no mortalities or toxicity signs were recorded in any of the experimental subjects. Based on the cytotoxicity experiment, the proposed nano-probe is considered safe and biocompatible.

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Section: Mechanism Of Quenchingmentioning

confidence: 99%

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

2020

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Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix^®

et al. 2014

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Fluorescence cystoscopy enhances detection of early bladder cancer. Water used to inflate thebladder during the procedure rapidly contains urine, which may contain fluorochromes. This frequently degradesfluorescence images. Samples of bladder washout fluid (BWF) or urine were collected (15 subjects). We studiedtheir fluorescence properties and assessed changes induced by pH (4 to 9) and temperature (15°C to 41°C).A typical fluorescence spectrum of BWF features a main peak (excitation/emission: 320∕420 nm, FWHM =50∕100 nm) and a weaker (5% to 20% of main peak intensity), secondary peak (excitation/emission: 455∕525 nm, FWHM = 80∕50 nm). Interpatient fluctuations of fluorescence intensity are observed. Fluorescence intensity decreases when temperature increases (max 30%) or pH values vary (max 25%). Neither approach is compatible with clinical settings. Fluorescence lifetime measurements suggest that 4-pyridoxic acid/riboflavin is the most likely molecule responsible for urine’s main/secondary fluorescence peak. Our measurements give an insight into the spectroscopy of the detrimental background fluorescence. This should be included in the optical design of fluorescence cystoscopes. We estimate that restricting the excitation range from 370–430 nm to 395–415 nm would reduce the BWF background by a factor 2.

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Diffusion through fat crystal networks

Li¹

2017

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Inner Filter Effect in the Fluorescence Emission Spectra of Room Temperature Ionic Liquids with-β-Carotene

Cited by 3 publications

References 22 publications

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix^®

Diffusion through fat crystal networks

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Inner Filter Effect in the Fluorescence Emission Spectra of Room Temperature Ionic Liquids with-β-Carotene

Cited by 3 publications

References 22 publications

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

Highly Luminescent and Biocompatible P and N Co-Doped Passivated Carbon Nanodots for the Sensitive and Selective Determination of Rifampicin Using the Inner Filter Effect

Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix®

Diffusion through fat crystal networks

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Product

Resources

About

Optical spectroscopy of the bladder washout fluid to optimize fluorescence cystoscopy with Hexvix^®