Innervation pattern of the deep extensor abdominal muscle in the crayfish species Astacus astacus

Adelsberger, Helmuth; Dudél, J.

doi:10.1007/bf00222787

Cited by 3 publications

(2 citation statements)

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“…6 and Table 2). These isomerizations into states from which neither binding of further GABA molecules nor openings can occur led to "napping channels"; as already suggested for former models ( [1,3], H. Adelsberger, unpublished results). The effect of a "blind end" is a depression of the Po and an increase of the rise time dependent on the degree of occupation of AR' or A2R'.…”

Section: Molecular Schemementioning

confidence: 63%

“…After cutting the segmental nerve innervating the DEAM, the inhibitory innervation was disrupted for the whole M-and L2 muscle bundle and the greater part of the L1-bundle which is not transsegmentally innervated from the anterior segment [1]. To exclude effects due to the transsegmental innervation, the outside-out patches were taken from the parts of the bundles close to the distal axon stump.…”

Section: Distribution and Density Of Chloride Channelsmentioning

confidence: 99%

See 1 more Smart Citation

Characterization and molecular reaction scheme of a chloride channel expressed after axotomy in crayfish

Adelsberger¹,

Beckerath²,

Dudél³

1996

Pflugers Arch - Eur J Physiol

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The nerve to the deep extensor abdominal muscle (DEAM) in crayfish species Astacus astacus, containing four excitatory and one inhibitory motor axons, was cut in the third segment on one side of the animal. The distal axon stump was not subject to phagocytosis but was present for months after the axotomy. The two lateral bundles of the DEAM were prepared 4-6 weeks after the axotomy. The gamma-aminobutyric-acid-(GABA-) activated chloride channel of these bundles was characterized by applying pulses of GABA to outside-out patches of the muscle membrane and measuring the responses. Based on the dose/response relationship of the peak current and of the rise time as well as on single-channel kinetics, a detailed molecular scheme for the reaction of the channel with GABA was derived. This scheme contains four binding steps of the agonist to the receptor and two open states. Simulations of the dose/response relationships with this model resulted in a set of rate constants which generate proper fits. In comparison to the channels present in innervated muscles, the channels of denervated muscles have a higher affinity for GABA, a lower single-channel conductance, four versus five binding steps, and non-cooperative binding. The first three of these adaptations of denervated muscles correspond to similar changes in denervated vertebrate muscles.

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Section: Molecular Schemementioning

confidence: 63%

Section: Distribution and Density Of Chloride Channelsmentioning

confidence: 99%