Innovative microwave-assisted iodized table salt or paper wastewater pretreatments for enhanced sugar recovery from banana pseudostem

Laltha, Milesh; Sewsynker-Sukai, Yeshona; Kana, E.B. Gueguim

doi:10.1007/s13399-022-02630-x

Cited by 3 publications

(4 citation statements)

References 36 publications

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“…The emergence of waste-based technologies for lignocellulosic CA production combined with simultaneous saccharification and fermentation (SSF) processes could reduce the time, expenditure for operation, energy input, risk of contamination and enhance the overall productivity and product yields. For instance, a Kraft paper wastewater pretreatment has been optimized on BP and demonstrated a high glucose yield (0.413 g/g) (Laltha et al, 2022). Similarly, fermentation medium constituents that are required to satisfy the nutritional needs of microbes are costly.…”

Section: Discussionmentioning

confidence: 99%

“…The Cellic CTec2 enzyme (160 FPU/mL) harnessed for the SSF processes was supplied by Novozymes (Novozymes A/S, Denmark). The paper wastewater (pH ~8) together with the safety data sheet containing its chemical composition (Laltha et al, 2022) was provided by a Kraft paper and pulp mill (Mondi, Richards Bay, South Africa). The dairy wastewater employed was obtained from a regional dairy (Fairfield, Howick, South Africa)…”

Section: Methodsmentioning

confidence: 99%

“…The powdered BP substrate was subjected to pretreatment using a previously optimized microwave-assisted paper wastewater method (Laltha et al, 2022). The BP substrate at a 30% (w/v) solid loading was submerged in 100 mL of paper wastewater and exposed to microwave heating at 800 W for 8 minutes.…”

Section: Pretreatment Of Banana Pseudostemmentioning

confidence: 99%

“…The BP substrate at a 30% (w/v) solid loading was submerged in 100 mL of paper wastewater and exposed to microwave heating at 800 W for 8 minutes. The pretreated BP biomass was filtered, washed and dried as stipulated by Laltha et al (2022), prior to being used in the SSF bioprocess. The composition of the optimally pretreated banana pseudostem was previously determined to contain cellulose (17.06%), hemicellulose (13.04%) and lignin (5.9%) (Laltha et al, 2022).…”

Section: Pretreatment Of Banana Pseudostemmentioning

confidence: 99%

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Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Laltha

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The bioconversion of renewable lignocellulosic biomass into value-added products such as citric acid (CA) has become a research hotspot towards a sustainable economy. Approximately 2 million tons of CA are produced annually with widespread use in the food, chemical, pharmaceutical and textile industries. Nevertheless, the use of lignocellulosic-derived feedstocks for CA production requires expensive, energy- and resource intensive pretreatment and fermentation processes, that result in low product yields. Therefore, the present study explores the valorization of banana pseudostem (BP) for CA production using the simultaneous saccharification and fermentation (SSF) process to alleviate these challenges. A review on the potential of BP as a feedstock for microbial production of value-added products is discussed together with the pretreatment and bioprocess challenges in addition to the possibilities to overcome these bottlenecks. Subsequently, two novel pretreatments consisting of a: (1) microwave-assisted-iodized table salt (M-ITS) and (2) microwave-assistedpaper wastewater (M-PWW) were modelled and optimized using the response surface methodology (RSM) for the enhancement of sugar recovery from BP. Then, the SSF process with pretreated BP was modelled and optimized using RSM for CA production. Thereafter, the logistic and modified Gompertz models were used to assess the kinetics of Aspergillus brasiliensis growth and CA production, respectively. The pretreatment input parameters for the M-ITS model comprised of salt concentration (1- 5%, w/v), microwave power intensity (100-900 W) and pretreatment time (2-10 min). On the other hand, the M-PWW model consisted of substrate solid loading (10-30% w/v), microwave power intensity (100-900 W) and pretreatment time (2-10 min). The output responses were reducing sugar yield (RSY) and glucose yield (GY) for both models. The optimal pretreatment conditions predicted by the M-ITS model were 2.48% ITS concentration, 800 W (power intensity) and 10 minutes (pretreatment time), corresponding to an experimental RSY (0.515 g/g) and GY (0.433 g/g). Pretreatment and optimization revealed a slightly higher RSY and GY for the M-ITS strategy when compared to the M-PWW method (RSY = 0.498 g/g and GY = 0.413 g/g). The M-PWW model predicted optimal pretreatment conditions of 30% solid loading, 800 W for 8 minutes. Despite the higher sugar yields observed for the M-ITS pretreatment, the M-PWW pretreatment represents a more suitable strategy owing to its complete waste-based approach that generates three times the quantity of pretreated substrate required for subsequent fermentation. SSF optimization included nitrogen (ammonium nitrate) concentration (0.5-5 w/v), desorbent (acetone) concentration (1-5% v/v) and temperature (30-40 °C) as inputs with CA concentration as the output (g/L). The optimized SSF conditions (0.5% w/v ammonium nitrate, 1% v/v acetone and 35 °C) (SSFoptimizedFW) gave a maximum CA concentration of 14.408 g/L. For the kinetic experiments, three bioprocesses consisting of the: (1) optimized SSF process with freshwater (SSFoptimizedFW), (2) SSFoptimizedFW process conditions while substituting dairy wastewater in place of freshwater (SSFDWW), and (3) Sabouraud Dextrose Emmon’s medium modified by substituting glucose with BP (SSFSDEmodified), were assessed. While all three bioprocesses gave the same maximum specific growth rate (μmax) of 0.05 h-1, the SSFSDEmodified process resulted in the highest maximum potential CA concentration (Pm) (13.991 g/L) compared to the SSFDWW (Pm= 13.095 g/L) and SSFoptimizedFW (Pm= 12.967 g/L) systems. The developed pretreatment strategies demonstrated glucose yields >0.40 g/g, shown to be higher than previously established chemical pretreatments. Moreover, the SSFDWW process displayed a slightly lower Pm value compared to the SSFSDEmodified strategy, and interestingly a higher Pm value than the SSFoptimizedFW bioprocess. Major findings from this study paves the way for lignocellulosic bioprocesses by potentially negating the use of costly pretreatment chemicals, fermentation medium constituents and/or fresh water, while achieving a “waste treating waste” approach. Thus, contributing to the water-energy-food nexus in line with global sustainable development goals towards a circular bioeconomy.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Pretreatment Of Banana Pseudostemmentioning

confidence: 99%

Section: Pretreatment Of Banana Pseudostemmentioning

confidence: 99%

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Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Laltha

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Effects of Particle Size on the Preparation of Biomass Samples for Structural Characterization

et al. 2023

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Comparative assessment of autoclave- and microwave-facilitated seawater pretreatments for the enhancement of sugar recovery from banana pseudostem

Hute,

Sanusi,

Kana

et al. 2024

Biomass Conv. Bioref.

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This study evaluated the efficacy of optimized autoclave-assisted seawater (A-SW) and microwave-assisted seawater (M-SW) pretreatments in improving sugar production from banana pseudostem. Subsequently, the structural changes of banana pseudostem, along with inhibitory compound concentrations were assessed under the conditions optimized for A-SW and M-SW pretreatment models. High coefficient of determination (R2) value (> 0.82) was obtained for both A-SW and M-SW. In addition, comparable reducing sugar of 0.41 ± 0.01 g/g and glucose yield of 0.24 ± 0.001 g/g were obtained from the optimized A-SW pretreatment, compared with the reducing sugar yield of 0.23 ± 0.004 g/g and glucose yield of 0.14 ± 002 g/g from the optimized M-SW regime. Furthermore, the A-SW high pretreatment efficiency was confirmed by changes in the biomass structure and low concentration of inhibitory compounds. Therefore, the potential use of abundant and sustainable seawater-based in this study with different heating mechanisms for pretreatment of lignocellulosic biomass provides a novel strategy to reduce the costs associated with the application of chemicals and scarce fresh water in lignocellulosic biorefineries.

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Innovative microwave-assisted iodized table salt or paper wastewater pretreatments for enhanced sugar recovery from banana pseudostem

Cited by 3 publications

References 36 publications

Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Development of innovative pretreatments for simultaneous saccharification and citric acid production from banana pseudostem: bioprocess optimization and kinetic assessment.

Effects of Particle Size on the Preparation of Biomass Samples for Structural Characterization

Comparative assessment of autoclave- and microwave-facilitated seawater pretreatments for the enhancement of sugar recovery from banana pseudostem

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