Innovative TLC-densitometric method with fluorescent detection for simultaneous determination of ternary anti-diabetic mixture in pharmaceutical formulations and human plasma

Eco-friendly micellar ultra-performance liquid chromatography (UPLC) method for simultaneous determination of non-communicable diseases drugs in bulk powder, spiked human plasma and in individual dosage forms

“…Different analytical methods have been published for the determination of GLM and PIO either alone or in combination including spectrophotometric [4][5][6][7], spectrofluorometric [8], HPLC [1,[9][10][11][12][13][14][15], TLC [1,16,17]. Several methods have been used for determination of VAL [2,[18][19][20][21][22][23][24][25][26][27], whereas ATV was determined by spectrophotometric [3,28], spectrofluorometric [29], HPLC [24,[30][31][32], TLC [33]and electrochemical [34] methods.…”

Simple TLC–spectrodensitometric method for studying lipophilicity and quantitative analysis of hypoglycemic drugs in their binary mixture

“…Salting‐out‐assisted thin‐layer chromatography (SOTLC) technique has been also used to study the retention and separation of solutes, and for the extraction and precipitation of protein in biological fluids mainly using ammonium sulfate (NH 4 ) 2 SO 4 salts (Novák & Havlíček, 2016; Qi, 2018). This technique is rapid, inexpensive and requires small sample volume; it does not need solvents with high purity because the impurities are already separated (Abbas et al, 2021; Ciura et al, 2016; Khashaba et al, 2017). In hydrophilic interaction chromatography, the presence of salts promotes retention of solutes by expanding the volume of the immobilized layer of water on the surface of the stationary phase, and polar solutes partition between this layer and the organic layer of the mobile phase (Alpert, 1990; Alpert, 2018).…”