Inoculation and Isolation of Plant Growth-Promoting Bacteria in Maize Grown in Vitória Da Conquista, Bahia, Brazil

Abstract

Maize is among the most important crops in the world. This plant species can be colonized by diazotrophic bacteria able to convert atmospheric N into ammonium under natural conditions. This study aimed to investigate the effect of inoculation of the diazotrophic bacterium Herbaspirillum seropedicae (ZAE94) and isolate new strains of plant growth-promoting bacteria in maize grown in Vitória da Conquista, Bahia, Brazil. The study was conducted in a greenhouse at the Experimental Area of the Universidade Estadual do Sudoeste da Bahia. Inoculation was performed with peat substrate, with and without inoculation containing strain ZAE94 of H. seropedicae and four rates of N, in the form of ammonium sulfate (0, 60, 100, and 140 kg ha -1 N). After 45 days, plant height, dry matter accumulation in shoots, percentage of N, and total N (NTotal) were evaluated. The bacteria were isolated from root and shoot fragments of the absolute control; the technique of the most probable number and identification of bacteria were used. The new isolates were physiologically characterized for production of indole acetic acid (IAA) and nitrogenase activity. We obtained 30 isolates from maize plants. Inoculation with strain ZAE94 promoted an increase of 14.3 % in shoot dry mass and of 44.3 % in NTotal when associated with the rate 60 kg ha -1 N. The strains N11 and N13 performed best with regard to IAA production and J06, J08, J10, and N15 stood out in acetylene reduction activity, demonstrating potential for inoculation of maize.

The Resurrection Plant Tripogon Spicatus (Poaceae) Harbors a Diversity of Plant Growth Promoting Bacteria in Northeastern Brazilian Caatinga

“…isolate ESA 0014. These results corroborate results from other studies, in which efficient diazotrophic and phytohormone producing bacteria were isolated from cultivated (Kuss et al, 2007;Ferrara et al, 2012;Silva et al, 2013;Santos et al, 2015) and wild species (Doty et al, 2009;Jha et al, 2012).…”

“…In addition to physiological mechanisms, plant tolerance to harsh environmental conditions can be influenced by plant association with soil plant growth promoting bacteria (PGPB), which are able to fix nitrogen release insoluble nutrients, secrete analogs to plant hormones, and control soil-borne pathogens, among other mechanisms (Kuss et al, 2007;Puente et al, 2009;Grover et al, 2011;Gumiere et al, 2014;Santos et al, 2015). Several studies that isolated and evaluated the diversity and biotechnological potential of PGPB in Brazil have already been carried out on crop species, mainly with grasses such as maize, rice, wheat, and sugar cane (Baldani et al, 2009;Hungria et al, 2010).…”

Triagem Do Potencial De Promocao Do Crescimento Vegetal De Rizobacterias Isoladas De Alface

“…Micro-organismos fixadores de nitrogênio são capazes de converter a forma gasosa do elemento, o nitrogênio atmosférico (N 2 ), em formas assimiláveis pelos vegetais como os nitratos e a amônia. (SANTI et al, 2013;SANTOS et al, 2015).…”

Adubação Biológica E Com Bioestimulante No Desenvolvimento

“…As bactérias contidas no produto utilizado como adubação biológica são percursoras de vários fitohormônios reguladores de crescimento de plantas, desenvolvendo funções importantes tais como produção de auxinas, etileno e amônia, solubilização de fosfatos, atividade antifúngica e solubilizadora de metais pesados (HAHN et al, 2013;AHEMAD;KIBRET, 2014;OLIVEIRA et al, 2015). Estes organismos crescem na rizosfera da planta estimulando o crescimento e desenvolvimento das mesmas por multiplicidade de mecanismos, funcionando como uma extensão do sistema radicular (SANTOS et al, 2015).…”