Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Vrieze, Jo De; Raport, Linde; Willems, Bernard; Verbrugge, Silke; Volcke, Eveline; Meers, Erik; Angenent, Largus T.; Boon, Nico

doi:10.1111/1751-7915.12268

Cited by 94 publications

(46 citation statements)

References 44 publications

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“…The average methane production during this period was about 355 L kg −1 VS. It should be noticed that in the work of De Vrieze et al a value of 363 L kg −1 VS was observed in batch reactors anaerobically treating molasses wastewater. The steady‐state conditions, as well as the methane production of about 98% of the aforementioned value, indicate that the sludge was already acclimated on day 62.…”

Section: Resultsmentioning

confidence: 94%

Start‐up and steady‐state results of a UASB reactor treating high pH baker's yeast molasses wastewater for methane production

Mischopoulou

Kalamaras

Naidis

et al. 2016

J of Chemical Tech & Biotech

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BACKGROUND The baker's yeast industry is a major market and due to the intensified production large quantities of waste are generated. Wastewater from the baker's yeast industry with high content of molasses was treated anaerobically in an upflow anaerobic sludge blanket (UASB) reactor for the production of methane (CH4). RESULTS The molasses wastewater obtained from industry had a high pH value around 8.0 ± 0.5, which remained constant during the experimental period. The system was operated at an organic loading rate (OLR) of 2.4–7.65 g of volatile solids per liter per day (g VS L−1 d−1) and the best results were observed at 4 g VS L−1 d−1, where methane production exceeded 350 L CH4 kg−1 VS. Anaerobic chemical oxygen demand removal was also examined and it was found that for the latter conditions of optimum OLR, it was more than 35%. VFA analysis revealed that acetic acid concentration in the reactor was more than 25 mmol L−1 even under optimum conditions. CONCLUSIONS The tested hydraulic retention time (HRT) values did not affect methane production, unlike the OLR. The increased acetic acid concentration in the UASB reactor and the high pH were credited to the inhibition of aceticlastic microorganisms. © 2016 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 94%

Start‐up and steady‐state results of a UASB reactor treating high pH baker's yeast molasses wastewater for methane production

Mischopoulou

Kalamaras

Naidis

et al. 2016

J of Chemical Tech & Biotech

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“…For example, spectra profiles of activated sludge from Min-Sheng WTP (Taipei, Taiwan) [10] and IDARS were different. Sludge from Min-Sheng WTP showed high content of total carbohydrates, and it was the main component among other organic substances.…”

Section: Discussionmentioning

confidence: 99%

Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

Kalneniece¹,

Bērziņš²,

Petrina³

et al. 2017

Materials Science and Applied Chemistry

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-The aim of this study was to characterize the digestate from an activated sludge biogas reactor (IDARS) as an inoculum for enzymatic hydrolysis and biogas production from organic wastes. Aerobic and anaerobic bacteria are represented in IDARS with H' index 3.1 and 2.5, respectively. IDARS stimulated the enzymatic hydrolysis of wood and leaves, preferably broadleaf tree species. The results demonstrated the potential of IDARS for improvement of the technological parameters for conversion of organic wastes into biogas.

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“…An active inoculum is crucial for fast and complete degradation of the substrate in a BMP test. Its impact has been demonstrated in previous studies (De Vrieze et al, 2015;Koch et al, 2017). It is not always possible to start an experiment right after collecting the inoculum, although inocula "should be used as fresh as possible" (Holliger et al, 2016).…”

Section: Impact Of Inoculum Storage On Smp Curvesmentioning

confidence: 99%

Identification of Critical Problems in Biochemical Methane Potential (BMP) Tests From Methane Production Curves

Koch

Hafner

Weinrich

et al. 2019

Front. Environ. Sci.

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Biochemical methane potential (BMP) tests are routinely conducted in academia and industry to determine the methane potential of a given substrate. Although many guidelines have been proposed to standardize BMP tests, results published in the peer-reviewed literature show that critical flaws in experimental design or execution are still common. Therefore, a powerful but simple method for evaluating the quality of BMP measurements is proposed in this work: the specific methane production (SMP) curve from any BMP trial should have a similar shape for most substrates, and significant deviation from this typical response is usually associated with a lack of reliability in resulting BMP estimates. In this study, some common experimental mistakes were reproduced to demonstrate this concept and establish relationships between flaws in experiments, SMP curves, and BMP values. The studied flaws were inoculum storage (2 weeks and at different temperatures), inoculum dilution with water (no dilution to 1:2 dilution), and inoculum-to-substrate ratio (from 2.00 to 0.05). Common kinetic models were used to better assess SMP curves. All flaws exhibited an impact on the SMP curves, but excessive dilution and extremely low inoculum-to-substrate ratio had the largest impacts on both SMP curves and BMP. In the most extreme case (ISR of 0.05), there was a clear lag phase of more than 10 days, and the resulting BMP was 15% lower than for the reference case. Together these results demonstrate the utility of the proposed method. The ultimate goal of this research is to help less experienced researchers identify and address problems in their experimental procedure, ultimately contributing to more accurate BMP measurements. The general approach is both simple and useful, and should always be part of a quality check for BMP tests.

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Inoculum selection influences the biochemical methane potential of agro‐industrial substrates

Cited by 94 publications

References 44 publications

Start‐up and steady‐state results of a UASB reactor treating high pH baker's yeast molasses wastewater for methane production

Start‐up and steady‐state results of a UASB reactor treating high pH baker's yeast molasses wastewater for methane production

Application of Anaerobic Digestate as Inoculum for Treatment of the Plant-Derived Wastes: Case Study

Identification of Critical Problems in Biochemical Methane Potential (BMP) Tests From Methane Production Curves

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