Inonotsuoxide B regulates M1 to M2 macrophage polarization through sirtuin-1/endoplasmic reticulum stress axis

Du, Na; Wu, Kun-Lung; Zhang, Jin; Wang, Lili; Pan, Xuesheng; Zhu, Yueqin; Wu, Xian; Liu, Jinghao; Chen, Yun; Ye, Ying; Wang, Yuan‐Yuan; Wu, Wenyong; Cheng, Wenming; Huang, Yan

doi:10.1016/j.intimp.2021.107603

Cited by 13 publications

(8 citation statements)

References 43 publications

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“…[ [18][19][20][21][22][23][24] Polyphenol Antioxidant [25] Triterpenoid Anticancer, anti-inflammatory, antiviral, and antioxidant [26][27][28][29][30] Melanin Antioxidant, anti-inflammatory, antiviral, hypolipidemic and immunomodulatory [24,[31][32][33][34] Polysaccharides are natural biopolymers that all living organisms have. And the diversity in monosaccharide composition and structure causes them to have different biological activities [35].…”

Section: Component Biological Activities Referencesmentioning

confidence: 99%

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Jia

Xue

et al. 2021

Polymers

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Inonotus obliquus (Chaga mushroom) is a kind of medicine and health food widely used by folk in China, Russia, Korea, and some occidental countries. Among the extracts from Inonotus obliquus, Inonotus obliquus polysaccharide (IOPS) is supposed to be one of the major bioactive components in Inonotus obliquus, which possesses antitumor, antioxidant, anti-virus, hypoglycemic, and hypolipidemic activities. In this review, the current advancements on extraction, purification, structural characteristics, and biological activities of IOPS were summarized. This review can provide significant insight into the IOPS bioactivities as their in vitro and in vivo data were summarized, and some possible mechanisms were listed. Furthermore, applications of IOPS were reviewed and discussed; IOPS might be a potential candidate for the treatment of cancers and type 2 diabetes. Besides, new perspectives for the future work of IOPS were also proposed.

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Section: Component Biological Activities Referencesmentioning

confidence: 99%

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Jia

Xue

et al. 2021

Polymers

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“…In recent years, research on M1/M2 differentiation of chaga extract has been explored, such as inonotsuoxide B, a tetracyclic triterpenoid extracted from chaga, was reported to have a regulation effect on macrophage polarization [ 44 ]. To the best of our knowledge, our current work was the first study on the effect of chaga crude polysaccharides on regulating M1 to M2 phenotype.…”

Section: Discussionmentioning

confidence: 99%

“…Further studies should probably focus on some purified compounds and their relationships with macrophage differentiation, such as inonotsuoxide B. The antitumor activity of inonotsuoxide B [ 48 ] and its regulation on macrophage polarization through sirtuin-1/endoplasmic reticulum stress axis [ 44 ] was explored.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Immunomodulatory Effects of Inonotus obliquus Extracts on Resting M0 Macrophages and LPS-Induced M1 Macrophages

Shen

Feng

Zhang

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Inonotus obliquus (Chaga) is a parasitic fungus that is distributed mainly in northeast China. Our literature research showed chaga polysaccharides have bilateral effects on tumor necrosis factor (TNF)-α and interleukin (IL)-1β levels when they exert antitumor and antidiabetic activities. The current research tried to explore the influence of chaga extracts on inflammatory factors via macrophage polarization which has bilateral immune-regulation not only on healthy tissue homeostasis but also on pathologies. Methods. Chaga was extracted with 100°C water and precipitated with 80% ethanol. The extracts were studied on RAW264.7 macrophage at resting condition (M0) and lipopolysaccharide (LPS)-activated subtype (classic activated macrophage, M1). The IL-1β, TNF-α, nitric oxide (NO) level, and the protein expressions of M1 and alternative activated macrophage (M2) markers including IL-1β, inducible NO synthase (iNOS), mannose receptor (CD206), and arginase (Arg)-1 were compared. Results. The 100 g extracts contained 13.7 g polysaccharides and 1.9 g polyphenols. Compared with M0, the 50 μg/mL extracts increased NO level ( P < 0.05 ) and decreased CD206 and Arg-1 expression significantly ( P < 0.05 ). The extracts at 100–200 μg/mL increased NO and TNF-α level ( P < 0.05 ), but increased iNOS and IL-1β expression significantly ( P < 0.05 ). Compared with M1, the extracts decreased NO level at 25, 50, 100, and 200 μg/mL and decreased IL-1β and TNF-α level at 100–200 μg/mL significantly ( P < 0.05 ). At 25–200 μg/mL, the extracts significantly increased CD206 and Arg-1 expression and decreased IL-1β and iNOS expression separately ( P < 0.05 ). Conclusions. Our research suggested that the bilateral effects of the chaga extracts on iNOS, IL-1β, and NO level on M0/M1 macrophages might be related with chaga polysaccharides and chaga polyphenols. Some in vivo anticancer and antidiabetic research of purified chaga polysaccharides related to macrophage differentiation should be conducted further.

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“…Chaga mushroom ( Inonotus obliquus ) comprises polyphenolic compounds and triterpenoids such as inotodiol [ 1 ]. These compounds have been reported to have broad bioactivities, such as cell cycle inhibition, anticancer activity, and anti-inflammatory effects [ 2 , 3 ]. As chaga mushroom gains increasing attention, its biological activities have been researched widely, and it has been uncovered to have antiviral, antioxidant, immunomodulatory, hypolipidemic, and hypoglycemic activities [ 4 , 5 , 6 , 7 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model

Nguyen¹,

Ban

Park³

et al. 2022

Molecules

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Chaga mushroom (Inonotus obliquus) comprises polyphenolic compounds, triterpenoids, polysaccharides, and sterols. Among the triterpenoid components, inotodiol has been broadly examined because of its various biological activities. The purpose of this study is to examine inotodiol from a safety point of view and to present the potential possibilities of inotodiol for medical usage. From chaga mushroom extract, crude inotodiol (INO20) and pure inotodiol (INO95) were produced. Mice were treated with either INO20 or INO95 once daily using oral administration for repeated dose toxicity evaluation. Serum biochemistry parameters were analyzed, and the level of pro-inflammatory cytokines in the serum was quantified. In parallel, the effect of inotodiol on food allergic symptoms was investigated. Repeated administration of inotodiol did not show any mortality or abnormalities in organs. In food allergy studies, the symptoms of diarrhea were ameliorated by administration with INO95 and INO20. Furthermore, the level of MCPT-1 decreased by treatment with inotodiol. In this study, we demonstrated for the first time that inotodiol does not cause any detrimental effect by showing anti-allergic activities in vivo by inhibiting mast cell function. Our data highlight the potential to use inotodiol as an immune modulator for diseases related to inflammation.

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Inonotsuoxide B regulates M1 to M2 macrophage polarization through sirtuin-1/endoplasmic reticulum stress axis

Cited by 13 publications

References 43 publications

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

In Vitro Immunomodulatory Effects of Inonotus obliquus Extracts on Resting M0 Macrophages and LPS-Induced M1 Macrophages

Evaluation of Toxicity and Efficacy of Inotodiol as an Anti-Inflammatory Agent Using Animal Model

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